Vanakripa minutiellipsoidea A. Pinnoi.

Yang, Hao, Dong, Wei, Yu, Xian-Dong, Bhat, D. Jayarama, Boonmee, Saranyaphat & Zhang, Huang, 2020, Four freshwater dematiaceous hyphomycetes in Sordariomycetes with two new species of Parafuscosporella, Phytotaxa 441 (1), pp. 19-34 : 29-31

Vanakripa minutiellipsoidea A. Pinnoi. Nova Hedwigia 77: 217 (2003) ( FIGURE 7 )

Index Fungorum number: IF489337

Saprobic on submerged wood. Sexual morph: undetermined. Asexual morph: Colonies on the natural substratum forming scattered, granular, black sporodochia. Mycelium mostly immersed in the substratum. Conidiophores micronematous, hyaline. Conidiogenous cells monoblastic, cylindrical, 4–5.5 μm wide. Separating cells cylindrical, clavate to vermiform, 27–45 × 5–13 μm (= 39 × 9 µm, n = 20), hyaline. Conidia ellipsoid to broadly clavate, dark brown to black, aseptate, 18–29 × 13–20 µm (= 25 × 17 µm, n = 30).

Cultural characteristics:—Conidia germinating on PDA within 36 h. Colonies on PDA slow-growing, reaching 2 cm diam. in a month at 20–25°C, floccose, rounded, sallow at first, becoming yellow when aged, with sparse mycelia on the surface, reverse yellow, with undulate margin. Hyphae aseptate, branched, hyaline, smooth-walled, 1–2.5 μm wide.

Material examined:— THAILAND. Chiang Mai Province: Mushroom Research Centre, 18°76’68’’N, 98°99’63’’E, on dead submerged decaying wood of an unidentified plant from freshwater, 1 September 2017, Xian-dong Yu, 11A ( MFLU 17–1724), living culture MFLUCC 17–2366.

Notes:— Vanakripa minutiellipsoidea was introduced by Pinnoi et al. (2003) and its phylogenetic placement was not established. The species is characterized by holoblastic, aseptate, dark brown to black conidia and clavate to vermiform, hyaline separating cells, which fits well with the generic concept of Vanakripa . Morphologically, our collection is identical to V.minutiellipsoidea ( Pinnoi et al.2003) .Phylogenetically,it clustered within V.minutiellipsoidea CBS 112523 ( MH 874467) with high support value (100%, FIGURE 1 ). There are 4 nucleotides differences in LSU sequences between our collection and the strain CBS 112523. Additionally, we provide ITS sequences.

Figures

FIGURE 1. Maximum likelihood majority rule consensus tree for Savoryellomycetidae based on a dataset of combined LSU, SSU, ITS and RPB2 sequence data. Bootstrap support values for maximum likelihood equal to or greater than 80% and Bayesian posterior probabilities equal to or greater than 0.95 are given above the nodes, respectively. The strain numbers are noted after the species names. Ex-type strains are in bold, newly generated sequences are indicated in blue, and new species are indicated in red. The tree is rooted with Leotia lubrica (AFTOL-ID 1) and Microglossum rufum (AFTOL-ID 1292). Orders are indicated as coloured blocks.

FIGURE 7. Vanakripa minutiellipsoidea (MFLU 17–1724) a, b Sporodochia on submerged wood. c, g–h Conidiogenous cells with conidia. d–f Conidia. i Germinated conidium on PDA. j, k Culture on PDA, j from above, k from reverse. Scale bars: c, d = 20 μm, e–i = 10 μm.