Spissiomyces endophytica N. Suwannarach, J. Kumla & S. Lumyong, 2018

Suwannarach, Nakarin, Kumla, Jaturong & Lumyong, Saisamorn, 2018, Spissiomyces endophytica (Dothideomycetes, Ascomycota), a new endophytic fungus from Thailand, Phytotaxa 333 (2), pp. 219-227 : 222-223

Spissiomyces endophytica N. Suwannarach, J. Kumla & S. Lumyong , sp. nov. ( Fig. 2 )

MycoBank: 822919

Etymology:— ‘endophytica’ is based on the word ‘endophyte’ from where the type strain was isolated.

Holotype:— THAILAND, Lampang Province, Mae Moh District , (18°40'52"N 98°52'11"E), isolated as an endophyte from tubers of Balanophora fungosa in a tropical deciduous forest, Jan, 2015, Suwannarach N., driedculture: SDBR-NKSpis016, living-culture: SDBR-CMU319.

Gene sequence:—MF990798 (SSU), MF990799 (LSU), MF990800 (ITS), MF990801 (RBP2) and MF990802 (TUB).

Fungal colonies on PDA, MEA, CMA and OA reaching 11.0±1.3, 10.8±0.6, 19.4±1.3 and 6.7±1.0 mm in diameter at 20 °C after two months of incubation ( Fig. 2A− D ). Colonies on PDA, MEA and OA compact, raised centrally, lobed, dark green to dark brown, and reveres dark green. Colonies on CMA, flat, raised centrally, lobed, brown and reveres dark green.A fungus produces a soluble bluish green pigment on PDA. On all agar media, mycelial composed spherical and torulose cells, yellowish brown, septate, thick-walled, 2−8.75 μm wide, intercalacy swollen cells, 10−16.5 μm and lateral germination ( Fig. 2E & F ). Conidiogenous cells, straight, cylindrical, 1.25−8.75 × 5−10 μm. Conidia, globose, pale brown, thick-walled, 10−17.5 μm ( Fig. 2G ).

Notes:—The larger conidial size and a soluble bluish green pigment production on PDA of this new species distinguishes it from other Spissiomyces species.

Key to species of Spissiomyces *

1a. Conidia absent .............................................................................................................................................. Spissiomyces aggregatus 1b. Conidia present...................................................................................................................................................................................2 2a. Conidia globose to ellipsoid, 4−10 μm ............................................................................................................ Spissiomyces ramosus 2b. Conidia globose, 10−17.5 μm .................................................................................................................... Spissiomyces endophytica

*Conidial production on malt extract agar in culture.

Figures

FIGURE 2. Spissiomyces endophytica SDBR-CMU319. A–D. Colonies on different agar media A. Potato dextrose agar. B. Malt extract agar. C. Cornmeal agar. D. Oatmeal agar. E. Hyphae and swollen cells (arrows). F. Hypal brancing and lateral germination. G. Conidiogenous cells (arrows) and conidia. Scale bars:A−D = 10 mm; E and G = 25 μm; F = 5 μm.