Sakaguchia melibiophila M. Groenew., Q.M. Wang, & F.Y. Bai, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 114

Sakaguchia melibiophila M. Groenew., Q.M. Wang, & F.Y. Bai sp. nov. MycoBank MB828817. Fig. 15E

Etymology: the specific epithet melibiophila refers to the physiological character of assimilating melibiose.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal, 2.5–4.4 × 3.8 –5.6 μm and single, budding is polar ( Fig. 15E ), a sediment is formed. After 1 mo at 17 °C, a sediment is present. On YM agar, after 1 mo at 17 °C, the streak culture is orange-red, butyrous, smooth. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, L-sorbose, cellobiose, trehalose, melibiose (delayed), D-xylose, L-arabinose, D-arabinose (delayed), D-ribose (delayed), ethanol, glycerol, ribitol, Dmannitol, salicin, D-glucitol, D-gluconate DL-lactate, succinate, citrate, myo-Inositol are assimilated as sole carbon sources. Sucrose, maltose, lactose, raffinose, melezitose, inulin, soluble starch, L-rhamnose, D-glucosamine, methanol, erythritol, galactitol and Methyl-α- D-glucoside are not assimilated. Potassium nitrate, sodium nitrite, L-lysine, ethylamine hydrochloride and cadaverine dihydrochlorideare assimilated as sole nitrogen sources. Maximum growth temperature is 35 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Sa. melibiophila differs from its closely related species Sa. lamellibrachiae and Sa. meli in its ability to assimilate cellobiose, melibiose, ribitol, and nitrate ( Table S1.23 ).

Typus: Netherlands, obtained from bronchial secretion, J. Swieringa (holotype CBS 5143 T preserved in a metabolically inactive state, ex-type JCM 8162 = CGMCC 2.4235).

Figures

Fig. 15. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Phy. aceris CGMCC 2.2662T; (C) Phy. jiayinensis CGMCC 2.5669T; (D) Me. layueensis CGMCC 2.5818T; (E) Sa. melibiophila CBS 5143T; (F) Mi. ellipsoideus CGMCC 2.5664T; (G, H) Mi. rubellus CGMCC 2.4444T; (I, J) Mi. pseudomagnisporus CGMCC 2.4538T; (K, L) Sy. rhododendri CGMCC 2.2613T; (M) Cy. raffinophilum CGMCC 2.3822T; (N) Cy. terricola CGMCC 2.3823T, (O) Do. ningxiaensis CGMCC 2.4451T; (P) Beg. foliicola CGMCC 2.3164T. Bars = 10 μm.