Ruinenia bangxiensis Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 109-110

Ruinenia bangxiensis Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828805. Fig. 14I, J .

Etymology: the specific epithet bangxiensis refers to the geographic origin of the type strain, Bangxi county, Hainan.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal and cylindrical, 2.2– 3.7 × 6.4–10.5 μm and single, budding is polar ( Fig. 14I ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is pinkish-orange, butyrous, wrinkled and dull. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are allantoid or reniform, 2.4– 2.9 × 5.3 –7.3 μm ( Fig. 14J ).

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, sucrose, maltose, cellobiose, trehalose, melibiose, raffinose, melezitose, inulin (variable), soluble starch (weak), D-xylose (weak), L-arabinose (delayed and weak), ethanol (variable), ribitol (variable), D-glucitol (variable), succinate (variable), and D-mannitol are assimilated as sole carbon sources. Galactose, L-sorbose, lactose, D-arabinose, Dribose, L-rhamnose, D-glucosamine, methanol, glycerol, erythritol, galactitol, Methyl-α- D-glucoside, salicin, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate and L-lysine (variable) are assimilated as sole nitrogen sources. Sodium nitrite, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 25 °C. Growth in vitamin-free medium is positive. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Ru. bangxiensis differs from its closely related species Ru. clavata in its inability to assimilate D-ribose and cadaverine and its ability to assimilate potassium nitrate ( Table S1.20 ).

Typus: China, Bangxi county, Hainan province, obtained from a leaf of an unidentified plant, Nov. 2006, Q.-M. Wang (holotype CGMCC 2.3454 T preserved in a metabolically inactive state, ex-type CBS 10819 = HBX1.0).

Figures

Fig. 14. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Kon. rhododendri CGMCC 2.3463T; (C, D) Ben. wuzhishanensis CGMCC 2.3569T; (E) Ben. pseudorectispora CGMCC 2.5677T; (F) Ps. fusiformis CGMCC 2.5823T; (G, H) Ru. fanjingshanensis CGMCC 2.4542T; (I, J) Ru. bangxiensis CGMCC 2.3454T; (K, L) Ru. lunata CGMCC 2.4426T; (M, N) Boe. sterigmata CGMCC 2.4539T, (O) St. layueensis CGMCC 2.5817T; (P) Pse. motuoensis CGMCC 2.5816T. Bars = 10 μm.