Pygmarrhopalites crepidinis Jordana & Baquero, 2017

Jordana, Rafael, Barranco, Pablo, Amezcua, Ana & Baquero, Enrique, 2017, Two new species of Collembola (Hexapoda) from Saliente Cave (Almería, Spain), Zoosystema 39 (1), pp. 103-115 : 106-112

Genus Pygmarrhopalites Vargovitsh, 2009

Pygmarrhopalites crepidinis Jordana & Baquero , n. sp. ( Figs 2 A-C; 3A-G, 4A-K; Table 1)

TYPE MATERIAL. — Holotype. ♀ in slide, date 21.IV.2013. Cueva del Saliente , Sierra de las Estancias, Cerro de la Ermita, Término Municipal de Oria (Almería, Spain), pitfall 08, slide CIA-MZ- 20130421a, Barranco-ECA leg.

Paratypes. Barranco-ECA leg.: CIA-MZ-20130421d, 1 ♀ on slide, 21.IV.2013, pitfall 08; MZ-20130421 e, 1♀ on slide, 21.IV.2013, pitfall 08 ; MZ-20130421 f, 1 ♀ on slide, 21.IV.2013, pitfall 08 ; MZ- 20130421g, 1 ♀ on slide, 21.IV.2013, pitfall 08 ; MZ-20130421 h, 1 ♂ (juvenile) on slide, 21.IV.2013, pitfall 07 ; MZ-20130421 i, 1 specimen on slide, 21.IV.2013, pitfall 09 ; MZ-20130421 j, 1 specimen juvenile on slide, 21.IV.2013, pitfall 02 ; CIA-MZ-20130421k and l, 2 ♀ on SEM stub, 21.IV.2013, pitfall 08; MZ-20130810 a, 1 ♀ on slide, 10.VIII.2013, pitfall 05 ; MZ-20130810 b, 1 ♀ on slide, 10.VIII.2013, pitfall 05 ; MZ-20130317 a, 1♀ on slide, 17.III.2013, pitfall 09; (deposited in MZ) . CIA-MNHN-20130421b, 1 ♀ in slide, 21.IV.2013, pitfall 08, 3 in ethyl alcohol 70% ( MNHN) ; CIA-MNCN-20130421c, 1♀ on slide, 21.IV.2013, pitfall 08, 3 in ethyl alcohol 70% ( MNCN) ; CIA-CECOUAL-20130317b, 2 ♀ on the same slide, 17.IIV.2013, pitfall 08; CIA-CECOUAL-20130321, 3 in ethyl alcohol 70% ( CECOUAL).

COMPLEMENTARY MATERIAL. — Cueva del Saliente (Barranco- ECA/AEV leg): CIA-MZ-20111218a, 1 specimen juvenile in slide, 18.XII.2011, pitfall 08 ; CIA-MZ-20111218b, 1 specimen juvenile in slide, 18.XII.2011, pitfall 02; CIA-MZ-20111218c, 1♀ on slide, 18.XII.2011, pitfall 07; CIA-MZ-20111218d, 1 ♀ on slide, 18.XII.2011, pitfall 09; CIA-MZ-20111218e, 1 specimen juvenile on slide, 18.XII.2011, pitfall 08b; CIA-MZ-20111218f, 1♀ on slide, 18.XII.2011, pitfall 04; CIA-MZ- 20111218g, 1 ♀ on slide, 18.XII.2011, pitfall 07. Cueva del Saliente (Barranco-ECA leg): CIA-MZ-20121201a, 1 specimen juvenile on slide, pitfall 07 ; CIA-MZ-20121201b, 1 ♀ on slide, 1.XII.2012, pitfall 06; CIA- MZ-20121201 c, 1 ♀ on slide, 1.XII.2012, pitfall 05 .

ETYMOLOGY. — The new species is named after Latin name of the cave: crepido, crepidinis .

DIAGNOSIS. — Unpigmented; 1+1 eyes; trichobothria A, B and C forming a straight line towards hind part; Ant. IV subdivided into five subsegments; all claws with inner tooth, all empodia with corner tooth, and empodial filaments overtopping tip of corresponding claw; anterior lobe of tenaculum with two apical chaetae; dens with 3, 2, 1…1 anterior chaetae, posterior side with one external spine; mucro gutter-like, both edges serrated, tip rounded; some circumanal chaetae broadened with wings but in some specimens with a basal spine in a3 without subbasal serration; anal appendices gutter-like, with apical and lateral end serration.

DESCRIPTION

General

Body length 1.05 mm (n =16) (holotype 1.22 mm), pigmentation absent. Habitus as Figure 2A .

Female

Head ( Fig. 3A, C ). Length 0.34 mm (n = 18). Eyes 1 + 1. No spine-like chaetae on the head.Clypeal area: 6 rows (from a to f); row a with axial chaeta, one axial chaeta between c, d and e rows. Inter-antennal area with 2 rows (α and β) without axial chaetae. Dorsal area: 4 rows (from A to D) with 4 axial chaetae in rows A, B, C and D; B without lateral chaetae ( Vargovitsh 2009). Labral chaetae number a: 4, m: 5, p: 5; prelabral: 6 ( Fig. 3C ). Labial palp with papillae A-E as in Figure 3E ( Fjellberg 1998); maxillary outer lobe with three sublobal chaetae, and palp with basal spine ( Fig. 3D ) ( Fjellberg 1984).

Antennae ( Fig. 4 A-D). 2 times as long as head (n =16). Ant. length 0.70 mm (n = 16), ratio of Ant segments I: II: III: IV = 1: 1.9: 3.0: 7.9 (average for n = 17). Ant. I with 7 chaetae, subapical posterior chaeta minute; Ant. II with 15 simple chaetae ( Fig. 4A ). Ant. III not swollen on sub-basal or medial part, with 17 chaetae and 2-rods sense organ; microsensillum Aai, chaetae Api and Ap straight, chaetae Ape, Ae and Ai curved ( Fig. 4B ). Ant. IV with 5 subsegments; subsegmental formula: 1+ 3 +1. AntIV bears the following whorls of chaetae: 5 on basal subsegment (BA, BM1-BM3, BB), 3 on medial subsegments each, and 5 on apical subsegment: AI-AIII, M1-M2 (after Vargovitsh, 2009) ( Fig. 4C, D ).Two species organites present and very patent on Ant. IV: subapical one with sphaeroidal big tip and apically special curved guard sensillum ( Fig. 4D )

Legs ( Fig. 4 E-J). Foreleg: precoxae 1, 2 and coxa with 1, 0, 1 chaetae respectively; trochanter with 3 anterior and 1 posterior chaetae; femur with 13 chaetae, a4 bending perpendicularly to the longitudinal axis of the segment ( Fig. 4E ) and pe 3 in a similar position; tibiotarsus ( Fig. 4F ) with 3 chaetae FP (FPe, FPae, FPpe) and chaeta FSa present; whorl I with 9 chaetae; whorls II-V with 8 chaetae each. Pretarsus with 1 anterior and 1 posterior chaetae. Claw with inner tooth and 2 pairs of lateral teeth visible in ventral view ( Fig. 4F ), tunica absent. Empodium thin, with corner tooth in subbasal half, with long apical filament exceeding tip of claw. Claw 4.5 times shorter than tibiotarsus. Mid leg: precoxae 1, 2 and coxa with 1, 1, 3 chaetae respectively; coxa with an additional spine; trochanter with 3 anterior simple chaetae, 1 posterior chaeta and anterior trochanteral organ; femur with 13 chaetae, 2 posterior ones smaller ( Fig. 4G ); tibiotarsus with 3 chaetae FP present, chaeta FSa present; whorl I with 9 chaetae, whorls II-III with 8 chaetae, whorl IV-V with 7 chaetae in different arrangement; claw broader than in foreleg, with inner tooth and 2 pairs of lateral teeth ( Fig. 4H ), tunica absent; empodium broader than in foreleg, with corner tooth in subbasal part; apical filament exceeding tip of claw; claw 5.5 times shorter than tibiotarsus ( Fig. 4H ). Hind leg: precoxae with 1, 1 chaetae and coxa with 3 chaetae and 1 small spine; trochanter with anterior trochanteral organ, 3 anterior and 1 posterior simple chaetae; femur with 10 anterior and 2 posterior chaetae ( Fig. 4I ); tibiotarsus with 3 chaetae FP, chaeta FSa present; whorls I-V as in mid tibiotarsus; claw broader than in fore and mid leg, with inner tooth and 2 pairs of lateral teeth ( Fig. 4J ); empodium broader than in the other legs, with corner tooth in the middle, and apical filament exceeding tip of claw; claw 7.5 times shorter than tibiotarsus ( Fig. 4J ). Length ratio of tibiotarsi I: II: III = 1: 1.1: 1.3.

Great abdomen ( Fig. 3A ). Meso- and metathorax with normal dorsal chaetae, 1 neosminthuroid chaeta a on Th II and III. Trichobothria A, B and C forming a straight line towards hind part. Single p chaeta of p-row of Abd. I is located below the level of B trichobothrium, chaeta a1 placed posterior to A trichobothrium, b1 placed between B and C trichobotria, chaeta c1 and c2 below C trichobothrium. Posterior lateral complex with 4+ 3 and furca base complex with 8 chaetae. Posterior dorsal complex with about 23 chaetae arranged in 3 rows. Ventral complex with 3 chaetae. Tenaculum with 3 teeth, a basal peg-like projection and 2 chaetae.

Furca . Manubrium with 5+5 posterior chaetae.Dens ( Fig. 4K ) with 3, 2, 1,..,1 anterior chaetae, 3 inner, 6 dorsal and 7 external chaetae; two external spines with variable morphology as Figure 4K (a, b, c) and 1 internal spine like chaeta (d).

Great abdomen. Abd. V with 2 chaetae and trichobothrium D in row a, and 3 chaetae in row p ( Fig. 3B ). Abd. VI ( Fig. 3F, G ) without cuticular spines; some circumanal chaetae broadened with wings usually smooth, sometimes with weak distal serrations or basal spur (a1 and a3); chaeta A1 shorter than chaeta a0 and DL2; subanal appendages (av5) gutter like, with apical serration.

Order ENTOMOBRYOMORPHA Börner, 1913 , sensu Soto-Adames et al. (2008) Family ENTOMOBRYIDAE Tömösvary, 1882 Subfamily ENTOMOBRYINAE Schäffer, 1896 Tribe LEPIDOCYRTINI Yoshii & Suhardjono, 1989 Genus Pseudosinella Schäffer, 1897

Pseudosinella najtae Jordana & Baquero , n. sp. ( Figs 2D, E ; 5 A-K)

TYPE MATERIAL. — Holotype. ♀, slide CIA-MZ-20130421a, Cueva del Saliente , Sierra de las Estancias , Municipality of Oria, Almería, 21.IV.2013, pitfall 07, Barranco Leg. (as the remaining specimens of type series).

Paratypes. CIA-MZ-20130421f to k, six specimens in ethyl alcohol, 21.IV.2013, pitfall 07; CIA-MZ-20130421l to u, 10 specimens in ethyl alcohol, 21.IV.2013, pitfall 06; CIA-MZ-20130421l to m, two specimens mounted in SEM stub, 21.IV.2013, pitfall 07; CIA-MZ- 20130421b, ♀ in slide, 21.IV.2013, pitfall 06; CIA-MZ-20130421c, juvenile in slide, 21.IV.2013, pitfall 06; CIA-MZ-20130810a, b and c, ♂, ♀ and juvenile in the same slide, 10.VIII.2013, pitfall 06; CIA-MZ-20130810d, ♀ in slide, 10.VIII.2013, pitfall 06; CIA-MZ-20130317b, juvenile in slide, 17.III.2013, pitfall 01; CIA-MZ-20130317c, juvenile in slide, 17.III.2013, pitfall 06 ( MZ). CIA-MNHN-20130421d, ♀ in slide, 21.IV.2013, pitfall 06 ( MNHN). CIA-CECOUAL-20130421e, ♀ in slide, 21.IV.2013, pitfall 06 ( CECOUAL). CIA-MNCN-20130317a, ♀ in slide, 17.VIII.2013, pitfall 04 ( MNCN) .

ETYMOLOGY. — The species name is dedicated to Judith Najt.

DIAGNOSIS. — Unpigmented; without eyes; chaetotaxy R0R1R2011/10/0100 + 2, without s accessory chaetae on Abd. IV, all claws with four teeth, paired teeth at different level (50% of claw length), all empodia with serrate external edge, labrum with all chaetae ciliated. Labium without M1, with M2rEL1L2, r is a smooth microchaetae, all labial chaetae both basal and internal ciliated.

DESCRIPTION

General

Maximum length 817 Μm, mean length 727 Μm (n = 8), (holotype 780 Μm) ( Fig. 2D ). Without pigment.

Head

Without eyes. Ratio antenna/cephalic diagonal 1.28, antennal segments I/II/III/IV ratios 1/1.5/1.4/2.8 (average n =7). Sensorial chaetae s on antennal segment III short, slightly curved and rod like ( Fig. 5D ) similar sensillae are present in external and lateral part of Ant. II and IV. Apical vesicle present on Ant. IV. Labrum chaetotaxy as in Figure 5A , all chaetae ciliated (SEM microphotograph in Figure 2E ). Formula of the labial base ( Fig. 5B ): MrEL1L2, all chaetae ciliated, r as smooth microchaetae. Antennae without scales.

Body chaetotaxy

Formula of the dorsal macrochaetae: R011/10/0100+2. R Mc complex with R0, R1 and R2. S (M) as microchaeta, T and S present as Mc ( Fig. 5C ). Abd. II chaetotaxy: -aBq1q2 ( Fig. 5J ), without p (a2p) chaeta, a2i as ciliated microchaeta, a2 as smooth microchaeta. B (m3) ciliated and long macrochaeta, as in normal location, a3 near and external to as. q1 (a3e) and q2 (p4) as smooth microchaetae. Abd. III with a2i, a6-a6ii as cilliated microchaetae, there are three am6 microchaetae (two smooth and one ciliated) ( Fig. 5J ). Accessory chaeta s in the anterior trichobothrial complex of abdominal tergite IV absent ( Fig. 5J ). P(C1) chaeta on abdominal tergite IV at level of anterior tricobothrium (T2) and as smooth microchaeta. Only B5 and B6 presents as ciliated Mc as Figure 5K . Pseudopore at level of B5. Retinaculum with 4+ 4 teeth and one ciliated chaeta.

Legs

Claw ( Fig. 5E ) with 4 teeth, basal pair of wing teeth at 50% of the base of inner edge, and different in size, medial unpaired tooth well developed, approximately 80% from base. Empodium acuminate with serrate external edge.Tenent hair clavate, longer than claw. Scales only on coxae.

Furca

Two internal and one external chaetae related to one distal pseudopore of manubrial plate. Mucro with apical tooth long as the apical; basal spine surpassing a little the tip of the anteapical tooth ( Fig. 5I ). Uncrenulated section of dens four times as long as mucro. Scales on manubrium and dens

Figures

FIG. 2. — A-C, Pygmarrhopalites crepidinis Jordana & Baquero, n. sp.: A, habitus; B, end of dens and mucro; C, dorsal view of claw of leg 3; D, E, Pseudosinella najtae Jordana & Baquero, n. sp.: D, habitus, E, Labrum detail. Scale bars: A, 300 μm; B, 30 μm; C, 9 μm; D, 200 μm, E, 4 μm.

FIG. 3.— Pygmarrhopalites crepidinis Jordana & Baquero, n. sp.: A, head chaetotaxy,nomenclature for head chaetae rows: A-C and D, vertex; α and β, interantennal, a, b, c, d, e and f, clypeal area; pl, prelabral row; B, body chaetotaxy; C, labrum chaetotaxy (pl, prelabral row; p, posterior; m, medial; a, anterior); D, maxillary outer lobe; E, labial papillae A to E (posterior papillae drawn separately); F, Abd. VI chaetotaxy; G, schematic posterior view of Abd. VI. Scale bars: A, F, G, 50 μm; B, 100 μm; C, D, 20 μm; E, 10 μm.

FIG. 4. — Pygmarrhopalites crepidinis Jordana & Baquero,n. sp.A, Ant.II; B, Ant.III;C, Ant.IV basal subsegment;D, Ant.IV distal four subsegments; E, subcoxae, coxa, trochanter and femur of leg 1, posterior view; F, tibiotarsus and claw of leg 1, posterior view; G, subcoxae, coxa, trochanter and femur of leg 2, anterior view; H, tibiotarsus and claw of leg 2, anterior view; I, subcoxae, coxa, trochanter and femur of leg 3, anterior view; J, tibiotarsus and claw of leg 3, anterior view; K, dens and mucro; a, b, c and d spines variations. Scale bars: 50 μm.

FIG. 5. — A-E, G, I-K, Pseudosinella najtae Jordana & Baquero, n. sp.: labrum showing the ciliated chaetae (A); labium chaetotaxy (B); head chaetotaxy (the nomenclature follows Gisin & Da Gama 1969, and in brackets Soto-Adames (2010) (C); Ant. II–IV (D); claw (E); male genital complex (G); dens end and mucro (I); Abd. II–IV chaetotaxy (J); Abd. IV macrochaeta B5 (K); F, H, Pseudosinella infrequens Gisin & Da Gama, 1969 (drawings from holotype): claw and empodium (F); dens end and mucro (H). Scale bars: A, 4 μm; B, 15 μm; C, D, G, I, 20 μm; E, 10 μm.