Psilocybe ningshanensis X.L. He, W.Y. Huo, L.G. Zhang, Y. Liu and J.Z. Li, 2022

He, Xue-Lian, Huo, Wen-Yan, Zhang, Li-Guang, Liu, Yu, Qi, Peng, Dai, Lu, Qiao, Ting, Lu, Pengpeng & Li, Jun-Zhi, 2022, Psilocybe ningshanensis (Hymenogastraceae, Agaricales), a new species from China, Phytotaxa 545 (2), pp. 175-185 : 177-178

Psilocybe ningshanensis X.L. He, W.Y. Huo, L.G. Zhang, Y. Liu and J.Z. Li , sp. nov. ( Figs. 2–4 )

GenBank MW939918 (ITS), MW939916 (LSU), MW939917 (SSU) and MycoBank MB839386

Diagnosis:—The species is characterized by a stipe or pileus turning bluish when bruised, absence of an annulus or veil, a yellowish brown to bright yellow, smooth, slightly sticky, shining pileus, yellowish to dark purple-brown lamellae, hexagonal basidiospores (6.5–) 8.5–10 (–11) × (4–) 5–6 (–6.5) μm, pleurocystidia and cheilocystidia clavateventricose to fusoid-clavate, sometimes sublageniform with slightly long, sinuous neck.

Holotype:— CHINA. Shaanxi Province: AnKang City, NingShan County, HuangHua Mountain , growing on woody debris in a subtropical deciduous broad-leaved forest, 1609 m elevation, N33°47’23.1”, E108°51’30.1”, 28 April 2020, W. Y. Huo, L. G. Zhang, Y. Liu et al. 20200025 (SXIM!).

Etymology:—‘ningshanensis’ refers to Ningshan County, where the species was discovered.

Description:— Pileus 20–30 mm diam, hemispherical when young, then convex to subumbonate with a depressed center when old, yellowish brown to bright yellow; smooth, glabrous, shining, slightly sticky, bluing in older specimen. Lamellae emarginate to emarginate with small tooth, slightly ventricose, moderately crowded, 1–2 tiers of lamellulae, yellowish when young to dark purple-brown at maturity; edges whitish when fresh. Stipe 40–50 mm × 3–5 mm, central, cylindrical, thicker at the apex and slightly enlarged at base, straight or flexuous, hollow, whitish to light yellow when fresh, pale brown when dry, fibrillose, base with white rhizomorphs. Annulus absent. Context whitish to pale yellowish. Turning bluish where bruised.

Basidiospores (6.5–) 8.5–10 (–11) × (4–) 5–6 (–6.5) μm, Q = 1.5–1.9, hexagonal with angles in frontal view, oval in side-view, with a wide apical germ pore, wall slightly thick to thick (0.8–1.2 μm), two layers in light microscopy, smooth, no reaction to Melzer’s, yellowish brown in KOH. Basidia 18–24 × 5–9 μm, clavate-ventricose, 4-spored, hyaline. Cheilocystidia (16–) 20–26 (–30) × (4.5–) 5.5–7.5 (–9.5) μm, common, clavate-ventricose to fusoidclavate, narrower near the apex, sometimes sublageniform with slightly long, sinuous neck, thin-walled, hyaline. Pleurocystidia (14.5–) 19–26 (–29) × (5–) 6–8.5 (–10) μm, like the cheilocystidia in form, hyaline. Subhymenium subcellular, composed of irregular round or polygonal cells, hyaline. Hymenophoral trama regular, hyphae 3.5–8 μm diam, thin-walled, hyaline. Pileus trama interwoven, hyphae 7–15 μm diam, thin-walled, hyaline. Pileipellis an ixocutis, consisting of parallel to interwoven hyphae in a dense gelatinous matrix 15–35 µm thick, hyphae 1–3 μm diam, filamentous, hyaline. Stipitipellis a cutis of parallel hyphae, 4–8 μm in diam, wall up to 0.5 μm thick, with pale yellowish contents. Caulocystidia not observed. Clamp-connections present in all tissues.

Habit, ecology and distribution:—Solitary on woody debris or decaying wood in the northern subtropical deciduous broad-leaved forest; so far known only from the HuangHua Mountain, China.

Additional specimen examined:— CHINA. Shaanxi Province: AnKang City, NingShan County, HuangHua Mountain , 1199 m elevation, 33°47’19.8”N, 109°03’32.9”E, 4 June 2020, X. L. He, W. Y. Huo, Y. Liu et al. 20200037 (SXIM!) .

Figures

FIGURE 2. a, b, c Fresh fruitbody of Psilocybe ningshanensis species (SXIM20200025); d, e, f Dried fruitbody (SXIM20200037). Scale bars: 1 cm. Photos by Y. Liu.

FIGURE 3. Microscopic features of Psilocybe ningshanensis in Congo Red. a, b, c Cheilocystidia; d, e, f Pleurocystidia. Scale bars: 25 µm. Photos by X.L. He.

FIGURE 4. Microscopic features of Psilocybe ningshanensis. a Basidia; b–c Basidiospores; d Pileipellis; a In Congo Red, b, c, d in 5 % KOH. Scale bars: a, b, d: 25 µm, c: 10 µm. Photos by X.L. He.