Pontibacter brevis, Osman & Gao & Wang & Mahmud & Sun & Zhang & Zhan & Zhang & Lou, 2018

Osman, Ghenijan, Gao, Yan, Wang, Ning, Mahmud, Otkur, Sun, Jian, Zhang, Tao, Zhan, Faqiang, Zhang, Zhidong & Lou, Kai, 2018, Pontibacter brevis sp. nov., isolated from rhizosphere soil of Tamarix ramosissima, International Journal of Systematic and Evolutionary Microbiology 68 (1), pp. 81-86 : 84-85

DESCRIPTION OF PONTIBACTER BREVIS SP. NOV.

Pontibacter brevis (bre′ vis. L. masc. adj. brevis short).

Cells are Gram-stain-negative, aerobic and oval-shaped (0.3–0.4 µm wide and 0.6 µm long), without flagella and non-motile. Colonies are light pink, circular, shiny, convex, smooth and 1–2 mm in diameter after 5 days incubation at 28 Ǫ C on 0.3×MB agar. Na + ions are not required for growth. Growth occurs at 5–37 Ǫ C (optimum 28–30 Ǫ C). Growth occurs in 0–8 % NaCl (optimum 0.5–2.0 %) and at pH 6.0–9.5 (optimum pH 7.0–8.0). Starch, aesculin and gelatin are hydrolysed but casein is not. Negative for nitrate reduction, indole production, glucose acidification, arginine dihydrolase, urease, H 2 S production, Voges–Proskauer test, lysine decarboxylase and ornithine decarboxylase. In the API ZYM test, positive reactions for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase, N -acetyl- β -glucosaminidase, trypsin, Oi -chymotrypsin, Oi -galactosidase, β -galactosidase and β -glucuronidase, but negative reactions for lipase (C14), Oi -mannosidase, β -fucosidase, Oi -glucosidase and β -glucosidase are observed. In the API 50 CH test, acid is produced from L- arabinose, ribose, D- xylose, galactose, D- glucose, D- fructose, D- mannose, L- rhamnose, methyl Oi- D- glucopyranoside, aesculin, salicin, cellobiose, maltose, lactose, melibiose, sucrose, trehalose, inulin, melezitose, raffinose, starch, glycogen, gentiobiose and potassium 5-ketogluconate, but not from glycerol, erythritol, D- arabinose, L- xylose, adonitol, β -methyl-D- xyloside, L- sorbose, dulcitol, inositol, D- manitol, D- sorbitol, methyl Oi- D- mannopyranoside, N -acetyl glucosamine, amygdalin, arbutin, xylitol, turanose, D- lyxose, D- tagatose, D- fucose, L- fucose, D- arabitol, L- arabitol, potassium gluconate or potassium 2-keto gluconate. Assays performed using the API 20NE system show no assimilation of D- glucose, L- arabinose, D- mannose, D- mannitol, N -acetyl-glucosamine, maltose, potassium gluconate, capric acid, adipic acid, malic acid, trisodium citrate or phenylacetic acid. In Biolog GNIII MicroPlate assays, positive reactions are observed for raffinose, Oi -D- glucose, D- sorbitol, gelatin, pectin, Tween 40, dextrin, lactose, D- mannose, D- galacturonic acid, maltose, melibiose, D- fructose, trehalose, β -methyl-D- glucoside, D- galactose, D- gluconic acid, cellobiose, D- salicin, L- aspartic acid, D- glucuronic acid, gentiobiose, N -acetyl-D- glucosamine, D- glucose-6-PO 4, L- glutamic acid, acetoacetic acid, sucrose, L- fucose, D- fructose-6-PO 4, propionic acid, turanose, L- rhamnose, quinic acid, acetic acid, stachyose and L- serine, but not for p -hydroxyphenylacetic acid, D- mannitol, glycyl-L- proline, methyl pyruvate, Ƴ -aminobutryric acid, D- arabitol, L- alanine, L- galactonic acid lactone, D- lactic acid methyl ester, Oi -hydroxybutyric acid, myo-inositol, L- arginine, L- lactic acid, β -hydroxy-D,L- butyric acid, 3-methyl glucose, glycerol, citric acid, Oi -ketobutyric acid, D- fucose, glucuronamide, Oi -ketoglutaric acid, N -acetyl- β -D- mannosamine, L- histidine, mucic acid, D- malic acid, N - acetyl-D-galactosamine, D- aspartic acid, L- pyroglutamic acid, L- malic acid, N -acetyl neuraminic acid, inosine, D- serine, D- saccharic acid, bromosuccinic acid and formic acid. The sole respiratory quinone is MK-7 and the major cellular fatty acids are summed feature 4 (iso-C 17: 1 I and/or anteiso-C 17: 1 B) and iso-C 15: 0. The major polar lipids are PE and two unidentified lipids.

The type strain is XAAS-2 T (=CCTCC AB 2016135 T =JCM 31443 T), isolated from the rhizosphere soil of Tamarix ramosissima collected from the shore of Aydingkol Lake , Xinjiang, China. The DNA G+C content of the type strain is 50.6 mol% .