Phylloporia nodostipitata Ferreira-Lopes & Drechsler-Santos, 2016

Luciorobledo, Gerardo, Reck, Mateusarduvino, Góes-Neto, Aristóteles & Drechsler-Santos, Elisandro Ricardo, 2016, Phylloporia spathulata sensu stricto and two new South American stipitate species of Phylloporia (Hymenochaetaceae), Phytotaxa 257 (2), pp. 133-148 : 142-144

Phylloporia nodostipitata Ferreira-Lopes & Drechsler-Santos sp.nov. ( Figures 2 C , 5 A–E )

Mycobank:—MB 805722

Diagnosis:—Basidiomata stipitate, seasonal to reviving, with brownish yellow pileus, tomentose pileal surface, knotted and irregularly flexuous stipe, 8–10(–12) pores p/mm and broadly ellipsoid basidiospores [2.5–3.5 × 2–3(–3.5) μm].

Etymology:— Nodostipitata (Lat. nodus) referring to the knotted and flexuous stipe.

Holotype:— BRAZIL. Santa Catarina: Florianópolis, Unidade de Conservação Ambiental Desterro , elevation 248 m. a. s. l., – 27° 31’ 52” S, – 48° 30’ 45” W, 31 th March 2012, E. R. Drechsler-Santos 801 ( FLOR! 51173). GenBank accession number ( LSU; KJ631412 ).

Description:—Basidiomata seasonal to reviving, stipitate, rarely solitary to caespitose with multiple pilei or a single, lobed pileus arising from a nodose mass of fused stipes; pileus circular, semi-circular to fan-shaped, flat to infundibiliform, up to 7.0 × 4.0 cm, new pilei may arise directly from the margin of an older one with no direct insertion of the stipe; pileus surface a cottony tomentum, shiny, concentrically zonate and sulcate, adpressed velutinate to scrupose, brownish yellow (10YR 6/6–6/8) to dark yellowish brown (10YR 3/6–4/6), in aged specimens the tomentum wearing away to expose semicircular dark streaks, tomentum yellowish brown in section (10YR 5/6–5/8), up to 0.5 mm thick, with a dark line up to 0.5 mm thick separating it from the context; context compact, appearing resinous in section, yellow (10YR 7/6–7/8), up to 0.5 mm thick; tube layer up to 1 mm deep, concolorous with the context, indistinctly stratified when reviving; margin rounded, entire to lobed, up to 2 mm thick, whitish when fresh, becoming yellowish brown (10YR 5/6–5/8) upon drying; hymenophore slightly decurrent, well delimited by a cottony sterile margin up to 1 mm thick, yellowish brown (10YR 5/6–5/8) becoming light olive brown (2.5Y 5/4–5/6) when mature, pores inconspicuous, circular to angular, 8–10(–12)/ mm, with spots of new tube layers growing irregularly upon older parts, dissepiments entire to slightly lacerated. Stipe attached to living roots, mostly prostrate on soil, often with several stipes entirely fused, eccentric to lateral, with a contracted base, strongly flexuous, nodose, uneven, up to 2.5 cm wide at the base and 7.0 cm high; stipe surface as a compressible tomentum, becoming hard and adpressed velutinate to scrupose with age, a thin dark line separating it from the context; stipe context compact, shiny, similar to the context of the pileus in color and texture.

Hyphal structure monomitic in all parts (truly monomitic sensu Corner 1991). Generative hyphae simple septate, slightly to moderately thick-walled, with wide lumen, sometimes with portions filled with a bright yellowish resin, pale to golden yellow, more frequent on the context and context of stipe; at the hymenophoral trama 2–3(–5) μm wide; at the context of pileus and stipe (3–)4–6(–7) μm wide; at the tomentum (pileus and stipe, Figure 5 A ) loosely interwoven, straight, mostly unbranched, 3–6(–7) μm wide, sometimes with local constrictions. Hymenium ( Figure 5 E ). Basidia 4-sterigmate, cylindrical to broadly clavate, thin-walled, hyaline in KOH, 10–15 × 4–5 μm. Basidioles spherical to barrel-shaped, thin-walled, hyaline in KOH, shorter. Cystidia rarely present, lageniform to subulate, hyaline in KOH, thin-walled, 17–20 × 3–5 μm. Basidiospores ( Figure 5 B–D ) broadly ellipsoid to ellipsoid in equatorial lateral view, subglobose in equatorial adaxial view, sometimes with either one flattened side on the equatorial or in the polar view, or in both; smooth; pale yellow in KOH/lactophenol; occasionally guttulate; thick-walled; 2.5–3.5 × 2–3(–3.5) μm (avg. = 3.0 × 2.5 μm), Q = 1.19–1.50, Q avg. = 1.33, IKI-.

Substrate and distribution:—Growing on living angiosperm roots; some specimens were connected to living roots probably of Psidium cattleyanum Sabine ( Myrtaceae Juss. ). So far known only from Brazilian Atlantic Forest Biogeographic Province (Santa Catarina State).

Additional specimens examined:— BRAZIL. Santa Catarina: Florianópolis, Unidade de Conservação Ambiental Desterro , elevation 248 m. a. s. l., 27° 31’ 52” S, 48° 30’ 45” W, growing on living roots, 25 th May 2013, V. Ferreira-Lopes 084 ( FLOR! 51259) ; Idem, Ibidem , 31 th March 2012, E. R. Drechsler-Santos 799 ( FLOR! 51171) ; Idem, Ibidem , E. R. Drechsler-Santos 800 ( FLOR! 51172) ; Idem, Ibidem , E. R. Drechsler-Santos 801 ( FLOR! 51173) ; Idem, Ibidem , E. R. Drechsler-Santos 802 ( FLOR! 51174) ; Idem, Ibidem , E. R. Drechsler-Santos 805 ( FLOR 51175 ) ; Idem, Ibidem , E. R. Drechsler-Santos 806 ( FLOR! 51176) ; Idem, Itapoá, Reserva Particular do Patrimônio Nacional Volta Velha , trilha do sambaqui elevation 20 m. a. s. l., 26º 04’ 56’’ S, 48º 38’ 56” W, 27 th February 2012, E. R. Drechsler-Santos 788 ( FLOR! 51168) ; Idem, Ibidem , 27 th February 2012, E. R. Drechsler-Santos 791 ( FLOR! 51170) ; Idem, Ibidem , 17 th February 2012, V. Ferreira-Lopes 79 ( FLOR! 51153) ; Idem, Ibidem , 17 th February 2012, V. Ferreira-Lopes 80, on living roots of Psidium cf. cattleyanum ( FLOR! 51154) ; Idem , ibidem, on living roots, 2 nd February 2013, A. C. Magnago 536 ( FLOR! 51254) ; Idem , ibidem, C. A. T. Oliveira 33 ( FLOR! 51237) .

Figures

FIGURE 2. A) P. spathulata (Holotype); B) P. elegans (Holotype); C) P. nodostipitata (FLOR 51154), on the upper-right corner, detail of P. nodostipitata pileus and stipe in section. Photographs by Gerardo L. Robledo (A, B) & Valéria F. Lopes (C).

FIGURE 5. Phylloporia nodostipitata microscopic features. A) pileus section with black arrow indicating the tomentum; B–D) Sporogram from light microscope observations of fresh specimens (FLOR 51173, FLOR 51154), representing three variations of the basidiospores in equatorial and polar view, with dotted lines representing flattened sides; E) Basidia, basidiole and lageniform cystidia, mature and young (el = equatorial lateral view; ead = equatorial adaxial view; eab = equatorial abaxial view; dp = distal polar view and pp = proximal polar view). Drawings by Gerardo L. Robledo & Valéria Ferreira Lopes.