Phaeoacremonium ovale S.K. Huang, R. Jeewon & K.D. Hyde

Huang, Shi-Ke, Jeewon, Rajesh, Hyde, Kevin D., Bhat, D. Jayarama, Putarak Chomnunti, & Wen, Ting-Chi, 2018, Beta-tubulin and Actin gene phylogeny supports Phaeoacremoniumovale as a new species from freshwater habitats in China, MycoKeys 41, pp. 1-15 : 4

Phaeoacremonium ovale S.K. Huang, R. Jeewon & K.D. Hyde sp. nov. Fig. 2

Type.

CHINA, Yunnan Province, Baoshan, stream along the roadside; saprobic on dead wood, 21 December 2016; Huang S.K. (KUN HKAS99550, holotype; MFLU MFLU18-1076, isotype); ex-type living culture (KUMCC 17-0145; KUMCC 18-0018). GenBank no. (ITS: MH399732, TUB: MH395327, ACT: MH395325; ITS: MH399733, TUB: MH395328, ACT: MH395326)

Etymology.

The name ovale refers to the oval shaped ascospores.

Description.

Sexual morph: Ascomata 225-300 μm (n = 5), on wood, perithecial, solitary, semi-immersed, unilocular, subglobose to globose, black, ostiolate, with ostiolar neck erumpent through bark of host when mature. Neck 445-645 × 35-45 μm (x̄ = 530 × 40 μm, n = 5), centrally ostiolate, contorted, lined with hyaline periphyses. Peridium 17-40 μm diam., membranous, composed of dark brown to hyaline cells of textura angularis. Hamathecium composed of 2-6 μm wide, hyaline, septate paraphyses, slightly constricted at septa and gradually narrowed towards apex. Asci 11-20 × 3-6 μm (x̄ = 15.5 × 5 μm, n = 30), 8-spored, unitunicate, clavate, with short pedicel, apically rounded. Ascospores 3-5 × 1.5-3 μm (x̄ = 3.5 × 2 μm, n = 50), bi-seriate, hyaline, oval to ellipsoid, aseptate, smooth-walled, rounded at the ends. Asexual morph: Mycelium on culture, partly superficial, composed of septate, branched, hyaline, rarely verrucose, hyphae 1.5-3 μm diam., rarely with adelophialides. Conidiophores usually arising from hyaline hyphae, mononematous, unbranched, occasionally constricted at basal septum, hyaline. Phialides 8-15 × 2-4 μm (x̄ = 9.5 × 3 μm, n = 20), terminal, monophialidic, elongate-ampulliform and attenuated at base. Conidia 2.5-6 × 1-2.5 µm (x̄ = 4 × 2 μm, n = 30), hyaline, ellipsoid to ovoid, aseptate.

Culture characteristics.

Ascospore germinating on PDA within 1 week at 23°C, germ tubes produced from ends. Colonies growing on PDA, reaching 2 cm diam. and sporulating after 30 days. Colonies semi-immersed to superficial, irregular in shape, flat, slightly raised, with undulate edge, slightly rough on surface, cottony to fairly fluffy, colony from above, greyish-brown (5F3-5, Ridgway 1912) at the margin, initially write to cream (5A1-3) in the centre, becoming dark brown (5F7-8) at the margin, orange-white (5B1-3) at the centre; from below, initially, greyish-brown at the margin, white at the centre, becoming dark brown at the margin, orange-white at the centre, producing brown pigmentation in agar.