Peziza koerberi PEYL, Lotos

Šandová, Markéta, 2015, Type Studies Of Several Species Of Lachnaceae (Ascomycota, Helotiales), Acta Musei Nationalis Pragae Series B 71 (3 - 4), pp. 399-412 : 403-404

Peziza koerberi PEYL, Lotos 8: 31, 1858.

Pl. 2, Figs 10–12, Pl. 5, Figs 1–24.

D e s c r i p t i o n. Material on? Cyperaceae (Pl. 2, Figs 10–12, Flagelloscypha ): Dried fruitbodies 0.1–0.45 mm in diam., outer surface whitish with dilute buff tinge (4D), covered with white hairs, often bearing a small circular brown lid (probably a part of plant tissue) up to 0.05 mm in diam. at the top of the fruitbody. Rehydrated fruitbody stalked, with 0.07 mm long, 0.05 mm wide stalk and 0.17 mm high, 0.18 mm wide cup. Hairs in water more than 120 µm long, 3.2–4.3 µm wide, hyaline to subhyaline, except for the basal part and mostly, except for the apical part, roughly warted (‘with crystalloids’), crystalloids up to 3(–3.3) µm long, apical part of hair without crystalloids 0–29 µm long, tapering, in various parts 1.3–3.0 µm wide, basal part of hair 2.2– 3.3 µm wide. In water, the hair wall in the apical part up to 1.0 µm thick, hair wall in the central part up to 0.9 µm thick, hair wall in the basal part 0.7 µm thick. Basidioles hyaline, with rounded apices, 13.5–23 × 3.8–5.7 µm, with basal clamp (KOH). Basidia were not recorded in the present study, but hopefully may be found during a detailed study of the specimen. Basidiospores hyaline, containing several lipid-bodies, 7.3–9.6 × 4.6–6.3 µm, average length 8.23 µm, average width 5.53 µm, l/w ratio 1.2–2.1 (KOH).

Material on grass (Pl. 5, Figs 1–11, Lachnella alboviolascens (ALBERTINI et SCHWEINITZ) FRIES ): Dried fruitbodies 0.3–0.52 mm in diam., 0.32–0.42 mm high, sessile, outer surface dilute fulvous to dilute cinnamon, covered with long white hairs, subiculum not present. Hairs in water 150– 230 µm long, 5.0–6.5 µm wide, hyaline, densely warted, hair wall 1.0–2.2 µm thick, basal parts of hairs in KOH hyaline or weak dilute buff (4D). Cells of surface tissue of frb. subhyaline, with dilute buff tinge, up to 4.2 µm wide in median section (water), the cells in surface view 10–17(–25) × 4.4– 5.7 µm (KOH). Cystidia hyaline, with rounded or asymmetrical apex, 38–42.5 × 5.3–6.1 µm, with basal clamp (KOH). Septate hymenial elements hyaline, with rounded or asymmetrical apex, upper cell of size 36.5–67 × 5.2–7.3 µm divided by a septum with no clamp (KOH). Basidia 104–109 × 10.5–11 µm (KOH). Sterigmata ca. 10 × 2.3 µm (KOH). Basidiospores hyaline, containing numerous lipid-bodies, 10– 13 × 6.5–9 µm, average width 7.34 µm, l/w ratio 1.41–1.68 (KOH).

Material on Pelargonium (reidentified here as Vitis, Pl. 5, Figs 12–24, Lachnella uvicola (SPEGAZZINI) COOKE ): Dried fruitbodies 0.25–0.57 mm in diam., sessile to short-stalked, surface of stalk pale ochre (5E to 6F), outer surface of cup dilute ochre (9H), covered with white hairs, subiculum not present. Hairs in water 150–170 µm long, 4–5.2 µm wide, hyaline, densely warted, hair wall 1.4–1.9(–2.3) µm thick, basal parts of hairs in KOH dilute buff-fulvous. Cells of surface tissue of frb. dilute fulvous (in water), elongated, 2.9– 4.4 µm wide (KOH). Cystidia or young hymenial elements rare, hyaline, with tapering to rounded apices, narrower than other elements, 30–37.5 × 4.2–5.6 µm, with basal clamp (KOH). Basidioles hyaline, with rounded to conical apices, (30–)41–70.5 × 6.8–8.6(–9) µm, with basal clamp (KOH); one exceptional element (probably immature basidium) of size 81 × 11.5 µm was seen. Basidia 44.7–52 × (8.7–)10.5– 12 µm (KOH). Sterigmata ca. 6 × 1–1.3 µm (KOH). Basidiospores hyaline, 11.6–13.2 × (7.5–)8.1–9.0 µm, average width 8.37 µm (KOH), 12.2–13.5 × 8.5–8.8 µm, average width 8.63 µm (MLZ), l/w ratio 1.35–1.72 (KOH), 1.44–1.53 (MLZ).

S p e c i m e n s s t u d i e d: the Czech Republic, Kačina near Kutná Hora, on? Cyperaceae , 1857, leg . J. Peyl , PRM 934750 (syntype). – the Czech Republic, in a greenhouse, on grass, January 1858, leg . J. Peyl , PRM 934749 (syntype). – the Czech Republic, in a greenhouse, on Pelargonium , leg . J. Peyl , PRM 727564 (syntype) .

D i s c u s s i o n. There was only one specimen of Peziza koerberi ( PRM 727564 ) housed in the PRM herbarium. During my examination, however, it became clear that the specimen contains material which is considered as belonging to three species, and that it includes different dates on Peyl’s original envelopes. Therefore the specimen has been divided .

The Pelargonium part was the larger part of the specimen. I found three pieces of grass culm among this material: two of them having a grass node, while one had a serrate leaf margin. These were given a new specimen number: PRM 934749. Additional material, probably containing leaves of Cyperaceae (leaf paremchyma, leaf epidermis and leaf veins) in two of Peyl’s envelopes labelled Kačin, 1857, is now recorded as specimen PRM 934750.

The size of basidiospores from the studied material located on Pelargonium (PRM 727564) best fits the size quoted for Lachnella uvicola by Agerer (1983). It also corresponds in the absence of a subiculum. Hair apices of L. uvicola were described as acute to subobtuse by Spegazzini (1899) – I also observed the same in Peyl’s material. According to Agerer (1983), Lachnella uvicola differs from L. villosa (PERSOON) DONK in lacking sterile elements in the hymenium (see also Piątek and Bujakiewicz 2004, Knudsen 2012 for L. villosa ). Irregular outgrowths beyond the hymenium surface are mentioned in the description of L. uvicola by Agerer (1983) and I also observed similar outgrowths in the studied material (Pl. 5, Figs 19, 21, 24). The substrate was considered as Pelargonium by Peyl and is here reidentified as Vitis (based on character of the bark and nodes).

The material on grass (PRM 934749) has smaller basidiospores compared with most modern literature, but based on the shape and size of the basidia, the material is considered here to be conspecific with Lachnella alboviolascens as described by Agerer (1983). A single basidiospore of 14.2 × 11.2 µm, not included to the description above, was seen, pushed between the hymenial tissue and cover glass (Pl. 5, Fig. 8).

The material on? Cyperaceae (PRM 934750) is considered here as belonging to Flagelloscypha . Detailed study is needed for a decision as to whether it differs from Flagelloscypha minutissima (BURT) DONK which was considered possible according to Agerer (1975). The material appears to be suitable for selection as the lectotype because of the substrate, Fimbristylis gracilis ( Cyperaceae ), which is quoted by Peyl in the protologue.

Trichopeziza adenostylidis (REHM) RAITVIIR , Eesti NSV Tead. Akad. Toim., Biol., 36(4): 317, 1987.

Pl. 6, Figs 1–10.

Basionym: Lachnum adenostylidis REHM, Ann. Mycol. 11(5): 392, 1913.

D e s c r i p t i o n. Dried apothecia 0.7–1.45 mm in diam, sessile, with saffron-orange to orange-apricot discs and saffron to ochre (9H) outer surface covered by white hairs. Hairs ca. 240–275 µm long, ca. 3.3–3.8 µm wide, ca. 13-septate (at least 11-septate), hyaline, with scattered warts. Asci 75.5– 80.5 × 4.2–5.1 µm, arising from croziers, ascus pore in MLZ: faintly blue, KOH/MLZ: blue, IKI: greyish blue, lower half of the channel greyish red or greyish blue, KOH/IKI: blue. Ascospores ca. 12–16 × 1.8–2.0 µm, 0(–1)-septate, germinating ascospores frequently 1-septate, OCI = 0–1(–2). Paraphyses lanceolate, 5–5.5 µm wide, exceeding the asci by 9.5–20(–26) µm.

S p e c i m e n s t u d i e d: Germany, Bayerische Alpen , im hinteren Wimbachtal am Watzmann, on decaying stems of Adenostyles alpina , July 1913, leg. Rehm , S ( F) 69053 (lectotype, designated here), Rehm, Ascomyc. Exs., no. 2059 .

Another specimen studied (not included in the description): same collection data, S(F) 69054, specimen from the exsiccate collection Rehm, Ascomyceten, no. 2059.

D i s c u s s i o n. According to the original description ( Rehm 1913), Lachnum adenostylidis is a species with large, sessile apothecia (2–3 mm in diam.) with white-yellowish discs and long (up to 300 µm) hyaline hairs. The examined material agrees with the original description. It possesses some characters which are not usual in Trichopeziza , especially the colour of the disc and the thick inner part of the medullary excipulum. Raitviir (1980) illustrated a similarly structured excipulum in Lasiobelonium nazarovae Raitv. The generic position of T. adenostylidis or L. nazarovae was, however, not investigated in the present study.

Belonidium adenostylidis (REHM) RAITVIIR ss. Raitviir (1970) and Schmid and Schmid (1991) is considered here as different from the type because the hairs are only up to 150 µm long, respectively 160 µm long, and discs bright ochraceous, respectively pale ochraceous, with an occasional pink tinge.