Pestalotiopsis nannuoensis C. Z. Yin, Z. X. Zhang & X. G. Zhang, 2024

Yin, Changzhun, Zhang, Zhaoxue, Wang, Shi, Ma, Liguo & Zhang, Xiuguo, 2024, Three new species of Pestalotiopsis (Amphisphaeriales, Sporocadaceae) were identified by morphology and multigene phylogeny from Hainan and Yunnan, China, MycoKeys 107, pp. 51-74 : 51-74

Pestalotiopsis nannuoensis C. Z. Yin, Z. X. Zhang & X. G. Zhang sp. nov.

Fig. 3

Type.

China, Yunnan Province, Menghai County, Nannuo Mountain (21 ° 55 ' 25 " N, 100 ° 35 ' 41 " E), from rotted leaves, 18 Mar 2023, C. Z. Yin, Z. X. Zhang and X. G. Zhang, holotype HMAS 352668 , ex-type living culture SAUCC 232203 .

Etymology.

Referring to the collection site of the holotype, Nannuo Mountain.

Description.

Conidiomata in culture on PDA, 750–900 µm diam, subsphaeroidal, solitary, black conidial masses permeated above the mycelium. Conidiophores mostly degenerated into conidiogenous cells, hyaline, simple. Conidiogenous cells oval, hyaline, rough, aggregative, 10.6–19.4 × 2.2–3.4 μm. Conidia fusiform or subcylindrical, straight or slightly curved, 4 - septate, 21.7–27.2 × 3.6–5.0 μm; basal cell conical, hyaline, rough, thin-walled, 3.9–5.4 µm; three median cells subcylindrical, brown, rough, thick-walled, the first median cell from base 4.4–6.2 μm, the second median cell 4.1–5.3 μm, the median third cell 4.5–5.7 μm, together 13.0–17.2 μm; apical cell conical or subcylindrical, hyaline, smooth, thin-walled, 2.9–4.6 µm; basal appendage tubular, single, centric, straight or slightly bent, unbranched, 6.8–9.2 µm; apical appendages tubular, 1–2, straight or bent, unbranched, 15.6–26.2 μm. Sexual morph not observed.

Culture characteristics.

After 7 days of dark cultivation at 25 ° C on PDA, the colony diameter reached 75 mm, and the growth rate is 9.5–11.5 mm / day. Colonies filamentous to circular, with filiform margin, aerial mycelium on surface rugged, white, dense, fruiting bodies black; reverse white.

Additional specimen examined.

China, Yunnan Province, Menghai County, Nannuo Mountain , from rotted leaves, 18 Mar 2023, C. Z. Yin, Z. X. Zhang and X. G. Zhang, living culture SAUCC 232204 .

Notes.

Pestalotiopsis nannuoensis sp. nov. formed an independent clade (ML / BI = 100 / 1) in the phylogenetic tree based on ITS, tub 2 and tef 1 α, and was closely related to P. diversiseta . P. nannuoensis was different from P. diversiseta by 46 / 508 bp in ITS, 83 / 529 bp in tub 2, and 59 / 465 bp in tef 1 α. Morphologically, P. nannuoensis was different from P. diversiseta by having shorter and thinner conidia ( P. nannuoensis : 21.7–27.2 × 3.6–5.0 vs. P. diversiseta : 27.0–34.0 × 5.5–8.0 µm), and the number of apical appendages ( P. nannuoensis : 1–2 vs. P. diversiseta : 3–5). ( Maharachchikumbura et al. 2012). Therefore, Pestalotiopsis nannuoensis was identified as a new species of Pestalotiopsis by morphological and phylogenetic comparison.

Figures

Figure 3. Pestalotiopsis nannuoensis (holotype: HMAS 352668) a, b the front and back of the colony after 14 days of culture on PDA c conidiomata on PDA d – f conidiophores and conidiogenous cells g – k conidia. Scale bars: 10 μm (d – k).