Paramyrothecium hydei J.R. Feng, Y. Chen & Y.L. Jiang, 2018

Feng, Jin-Rong, Wang, Yong, Yang, Bin, Bhat, D. Jayarama, Chen, Yun, Ran, Shuang-Fei, Xu, Ying & Jiang, Yu-Lan, 2018, Species of Stachybotryaceae in China, Phytotaxa 346 (3), pp. 258-268 : 262

Paramyrothecium hydei J.R. Feng, Y. Chen & Y.L. Jiang , sp. nov. Fig. 3

MycoBank: MB822863; Facesoffungi number: FoF 04190

Etymology: In reference to Dr Kevin D. Hyde, who provided outstanding contribution to this fungal group.

Isolated from soil. Sexual morph: Undetermined. Asexual morph: coelomycetous.

Colonies with abundant white to rosy-buff aerial mycelium, flat, black, with white regular margin, covered by slimy olivaceous green conidial masses, reverse white, 4–6 cm diam. in one week on PDA at 25 ºC. Conidiomata sporodochial, rarely scattered or always gregarious, stromatic, superficial, cupulate, with a white setose fringe surrounding an olivaceous-green agglutinated slimy mass of conidia. Stroma poorly developed, hyaline, of textura angularis. Conidiophores arising from the basal stroma, consisting of a stipe and a penicillately branched conidiogenous apparatus; stipes unbranched, hyaline, aseptate, smooth, 12–20 × 1–2 μm; primary branches aseptate, unbranched, smooth, 5.5–11 × 1–2 μm; secondary branches aseptate, unbranched, smooth, 6–9 × 1–2 μm; terminating in a whorl of 3–6 conidiogenous cells. Conidiogenous cells phialidic, cylindrical to subcylindrical, hyaline, dark at the collarettes, smooth, straight to slightly curved, 7–13 × 1–1.5 μm (av. 9.4 × 1.1 μm), with conspicuous collarettes and periclinal thickenings. Conidia aseptate, hyaline, smooth, cylindrical to ellipsoidal, 4.5–6 × 1–2 μm (av. 5.3 × 1.4 μm), rounded at both ends.

Type: CHINA, Guizhou, Guiyang City, Huaxi District, from soil, 4 November 2016, Jin-Rong Feng ( HGUP 8077, holotype), ex-type culture GUCC 6077.

Other material examined: CHINA, Guizhou Province, Guiyang City, Huaxi District, from soil, 4 November 2016, J. R Feng ( HGUP 8098), living culture GUCC 6098.

Notes: Phylogenetic analyses based on three phylogenetic markers ( ITS, tub2 and CAL) showed that our two strains clustered together with the same DNA sequences in three gene regions supported by high bootstrap values ( MP: 100% and ML: 100%), and formed a branch with P. breviseta , P. follicola and P. roridum ( MP: 91% and ML: 69%). In morphology, conidia of our strains are smaller than those of P. roridum (6.5–7.5 × 2 μm), but some smaller than P. follicola (5–6 × 1–2 μm). The conidiophores of P. hydei are longer than those of P. breviseta (11–17 × 2–3 μm), but shorter than those of P. follicola (15–25 × 2–3 μm) and P. roridum (15–40 × 2–4 μm). The conidiogenous cells of P. hydei are obviously smaller than those of P. roridum (7–33 × 2–3 μm), but larger than P. breviseta (6–11 × 1–2 μm). Thus, combining morphology and phylogeny we proposed that HGUP 8077 and 8098 represent a new taxon.

Figures

FIGURE 3. Paramyrothecium hydei (HGUP 8077). a-c Olivaceous-green sporodochial conidiomata on PDA. d Sporodochial conidiophores. e Conidiogenous cells. f Conidia. Scale Bars: d, e = 10 μm, f = 5 μm.