Nothogreniera fergusoni ( Tonnoir), 1925

A. Craig, Douglas, Currie, Douglas C. & Moulton, John K., 2018, Nothogreniera new genus, for two species of Australian " Paracnephia " (Diptera: Simuliidae), Zootaxa 4444 (4), pp. 351-380 : 353-368

Nothogreniera fergusoni ( Tonnoir), 1925 . New combination

( FIgS. 1–49 )

Simulium fergusoni Tonnoir 1925: 238 . Original description, female only.

Simulium fergusoni . Smart 1945: 505. Overview of Simuliidae . (As Sharp[sic] in Mackerras & Mackerras 1949: 386).

?Unidentified simuliid. Wharton 1949: 411. Larvae—probably fergusoni .

Cnephia fergusoni (Tonnoir) . Mackerras & Mackerras, 1949: 386. New combination; terebrans -species group.

Cnephia fergusoni (Tonnoir) . Grenier, 1960: 739.

( Cnephia of authors) fergusoni . Crosskey, 1987: 443. Prosimuliini , undetermined genus.

Paracnephia fergusoni (Tonnoir) . Crosskey & Howard, 1997: 18, 117. New combination, unplaced to subgenus.

Paracnephia fergusoni . Bugledich, 1999: 328. Compilation of data.

“ Cnephia ” ‘S. x. (east)’. Moulton, 2000: 98. 2003: 47.

Paracnephia fergusoni . Crosskey & Howard, 2004: 10. Unplaced to subgenus. Adler & Crosskey, 2008: 26. Unplaced to subgenus, transferred to Simuliini : 16. Adler & Crosskey, 2018: 30.

Nothogreniera fergusoni (Tonnoir) . New combination, this paper.

Description. Adult female (BASED ON THREE PARATyPES AND TWO PHARATE SPECIMENS). Body: HEAD AND THORAX DARK, MARKEDly POllINOSE IN SOME lIgHTINg, PRODUCINg gREyISH-SIlVERy APPEARANCE ( FIgS. 1, 3 —IN AlCOHOl), WITH DARK BROWN AND yEllOWISH ABDOMEN; TOTAl lENgTH ca. 1.5 MM. Head ( FIg. 5 ): FRONS TAPERED ONly SlIgHTly TOWARDS ANTENNAE, NARROWEST JUST ABOVE ANTENNAE; WIDTH 0.71–0.79 MM; DEPTH 0.5 9–0.63 MM; POSTOCCIPUT BlACK, VESTITURE OF SPARSE, lONg BlACK HAIRS; FRONS, DARK BROWN-BlACK, VESTITURE OF MARKEDly SPARSE SIlVERy HAIRS; FRONS / HEAD RATIO 1.0:5.5–1.0:4.7. Eyes: INTEROCUlAR DISTANCE 0.16–0.17 MM; OMMATIDIA DIAMETER 0.016 MM; ca. 37 ROWS ACROSS AND 47 DOWN AT MID-EyE. Clypeus: BROAD, WIDTH 0.25–0.28 MM; SIlVERy, MODERATE VESTITURE OF FINE SIlVERy HAIRS. Antenna ( FIg. 6 ): TOTAl lENgTH 0.75–0.9 MM, EXTENDED MORE THAN HAlF lENgTH BEyOND HEAD MARgIN; SCAPE SMAll, PEDICEl BROADER DISTAlly, NINE FlAgEllOMERES, FIRST BROAD, REMAINDER BROADER THAN lONg, DISTAl FlAgEllOMERE SMAll, TAPERED, lONgER THAN BROAD; ANTENNA OVERAll SlIgHTly TAPERED. Mouthparts: ca. 0.5X lENgTH OF HEAD DEPTH; CIBARIUM ( FIg. 9 ) CORNUAE BROADly FlARED AND MODERATEly SClEROTIZED, MEDIAN DEPRESSION FlAT-BOTTOMED; MANDIBlE ( FIg. 8 ), POINTED APICAlly WITH ca. 16 OUTER AND 41 INNER TEETH; lACINIA WITH ca. 20 TEETH ON EITHER SIDE; MAXIllARy PAlP ( FIg. 7 ), TOTAl lENgTH 0.54 MM, PAlPOMERES I AND II SMAll, III DARKER BROWN THAN REMAINDER, ONly SlIgHTly EXTENDED BEyOND ARTICUlATION WITH IV, THAT RElATIVEly SHORT, V NOT REMARKABly ElONgATE; PROPORTIONAl lENgTHS OF III–V PAlPOMERES 1.0:0.7:1.1; SENSORy ORgAN ElONgATED, 0.5X lENgTH OF PAlPOMERE III, OPENINg 0.5X WIDTH OF VESIClE. Thorax: lENgTH 1.5 MM; WIDTH 0.9–1.2 MM; POSTPRONOTAl lOBE WEll DEVElOPED, WITH DENSE FINE HAIR lONgER THAN ON SCUTUM, THAT SHOWINg DENSE POllINOSITy IN SOME lIgHTINg ( FIg. 1 ), OVERAll WITH EVEN SPARSE FINE SMAll HAIRS; SCUTEllAR DEPRESSION SIMIlAR; SCUTEllUM SlIgHTly PAlER THAN SCUTUM, VESTITURE OF SPARSE FINE yEllOWISH HAIRS; POSTNOTUM CONCOlOUROUS WITH SCUTEllUM, VESTITURE SIMIlAR; ANTEPRONOTAl lOBE (AKA PROPlEURON) AND PROEPISTERNUM WITH lONg FINE yEllOWISH HAIRS; FORE COXA HAIRED; PlEURON AND ANEPISTERNAl MEMBRANE lACKINg HAIRS ( FIg. 10 ). Wing ( FIgS. 11 , 12 ): lENgTH 3.0–3.3 MM; WIDTH 1.5–1.7 MM; SMAll BASAl CEll PRESENT; A/B RATIO FROM 1.0:2.6 TO 1.0:2.7. COSTA lACKINg SPINE-lIKE SETAE; RS NOT BRANCHED, CUA MARKEDly SINUOUS, M1 APPEARS DOUBlE (DISTINCT ARRANgEMENT OF MICROTRICHIA PlUS FOlD). Haltere: OVERAll lIgHT BROWN. Legs ( FIg. 13 ): EVENly MEDIUM BROWN; HIND BASITARSUS WITH VENTRAl ROW OF STOUT SPINES, ClUSTERED AT POSTEROVENTRAl TERMINUS OF BASITARSUS; CAlCIPAlA ABSENT; DISTINCT HIRSUTE INTERSEgMENTAl PlATE VENTRAlly BETWEEN BASITARSUS AND TARSOMERE II; PEDISUlCUS ABSENT; TARSOMERE II 4X lONgER THAN BASAl WIDTH; ClAWS SMAll ( FIg. 14 ), TAlON ONly SlIgHTly CURVED, NOT OBVIOUSly SERRATED, BASAl TOOTH POORly EXPRESSED, lATERAl OF TAlON AND VARIABlE EVEN ON SAME SPECIMEN, ROUNDED HEEl MODERATEly DEVElOPED. Abdomen ( FIg. 15 ): BASAl SCAlE DARK BROWN, VESTITURE OF lONg DENSE yEllOW HAIRS; REMAININg SEgMENTS DARK BROWN, TERgITES yEllOWISH BROWN, SlIgHTly SHINy; TERgITE II BROADly BOWl-SHAPED, DARK, TERgITES III–VI ROUNDly RECTANgUlAR, SUBEQUAl IN SIZE; VESTITURE OF MARKEDly SPARSE, SMAll SIlVERy HAIRS INCREASINg IN lENgTH AND DENSITy POSTERIORly, REMAININg TERgITES BROADER; PlEURITES MODERATEly DEVElOPED; STERNITES APPARENTly ABSENT. Genitalia: STERNITE VIII MORE-OR-lESS EVENly PIgMENTED ACROSS FUll WIDTH, VESTITURE OF ROWS OF MICROTRICHIA, lARgE STRONg HAIRS POSTEROlATERAlly; HyPOgyNIAl VAlVES ( FIg. 19 ), lIgHTly PIgMENTED, VESTITURE OF TRIADS OF MICROTRICHIA, MEDIAl EDgES OF VAlVES CONVEX, SlIgHTly STRENgTHENED; gENITAl FORK ( FIg. 18 ) ANTERIOR ARM NARROWED, DISTINCT, APEX SlIgHTly EXPANDED, MEMBRANOUS lATERAl AREAS, lATERAl ARMS WEll EXPRESSED, POORly SClEROTIZED, APODEME BAREly OBVIOUS AS RAISED RIDgE AT JUNCTION OF ARM AND POSTEROlATERAl EXPANSION, THAT ROUNDED lATERAlly, RECTANgUlAR MEDIAlly; CERCUS IN lATERAl VIEW BROADly ROUNDED APICAlly WITH SlIgHT INDENTATION, TENDINg TO FlAT AlONg DORSAl MARgIN, ANAl lOBE SUBSTANTIAl ( FIg. 20 ); SPERMATHECA OVOID, EXTERNAlly SMOOTH WITH INTERNAl FINE SPINES ( FIg. 16 ) NOT OBVIOUS IN FUlly MATURE EXAMPlES ( FIg. 17 ), SPINE lENgTH ca. 7.1 µM, WIDTH ca. 0.47µM, lENgTH TO WIDTH RATIO ca. 1:5, DENSITy 0.055/µM2, REgION SURROUNDINg JUNCTION WITH SPERMATHECAl DUCT SCUlPTED, NOT MARKEDly ClEAR.

Adult male ( REARED INDIVIDUAlS AND PHARATE SPECIMENS). Body: IN ETHANOl, OVERAll COlOUR BlACK; TOTAl lENgTH 3.8 MM. Head (FIg. 21) : WIDTH 1.1 MM; DEPTH 0.8 MM. Eyes : UPPER OMMATIDIA DARK REDDISH BlACK, DIAMETER 0.029 MM, ca. 30 ACROSS AND 27 DOWN ; lOWER OMMATIDIA BlACK, MARKEDly SMAll, DIAMETER 0.007 MM, ca. 35 ACROSS AND 44 DOWN ; lARgER OMMATIDIA OVERHANg SMAllER OMMATIDIA AT JUNCTION. Clypeus : BlACK ; WIDTH 0.17 MM; VESTITURE OF SPARSE FINE BlACK HAIRS. Antenna (FIg. 22): TOTAl lENgTH 0.79 MM; OVERAll BlACK, PEDICEl SMAll , SCAPE TWICE AS lONg AND ROUNDED, ANTENNOMERE I SlIgHTly NARROWER THAN SCAPE, REMAININg ANTENNOMERES TAPERED SlIgHTly TO SMAll APICAl ANTENNOMERE. Mouthparts: INSUBSTANTIAl; lENgTH 0.3X HEAD DEPTH ; MAXIllARy PAlPUS (FIg. 23) 0.65 MM lONg, PAlPOMERES I AND II SMAll, PROPORTIONAl lENgTHS OF III–V PAlPOMERES 1.0:0.7:1.1, SENSORy VESIClE OF IRREgUlAR SPHERICAl SHAPE, OCCUPyINg 0.45X WIDTH OF PAlPOMERE III, OPENINg 0.3X VESIClE WIDTH. Thorax: lENgTH 1.4 MM; WIDTH 0.96 MM; POSTPRONOTAl lOBE, ANTEPRONOTAl lOBE AND PROEPISTERNUM HAIRED; SCUTUM EVENly VElVETy BlACK, VESTITURE OF SPARSE SHORT FINE PAlE HAIRS, DENSE AND lONg IN SCUTEllAR DEPRESSION; SCUTEllUM CONCOlOUROUS WITH SCUTUM, BARE MEDIAlly WITH SPARSE lONg PAlE HAIRS lATERAlly; POSTNOTUM CONCOlOUROUS WITH SCUTUM; PlEURAE DARK BROWN, ANEPISTERNAl MEMBRANE lACKINg HAIRS. Wing: lENgTH 3.4 MM, WIDTH 1.6 MM, OTHERWISE AS FOR FEMAlE. Haltere : BASE OF STEM BlACK, KNOB DARK TAN. Legs : FRONT lEg BlACK, FEMUR WITH MARKEDly lONg FINE HAIRS; MID lEg MAINly BlACK TO BlACKISH BROWN; HIND lEg WITH PROXIMAl SEgMENTS HIRSUTE, BlACKISH BROWN, BASITARSUS DARK BROWN AS ARE TARSOMERES; BASITARSUS WITH DENSE SHORT SPINE-lIKE HAIRS ON PROXIMAl 2/3, VENTRAl ROW OF STOUT SPINES PRESENT, CAlCIPAlA ABSENT, HOWEVER THE SMAll, HAIRy, VENTRAl, INTERSEgMENTAl SClERITE MAy APPEAR AS A POORly DEVElOPED CAlCIPAlA; PEDISUlCUS ABSENT ; TARSAl ClAW gRAPPlINg PAD OF ca. 25 TEETH. Abdomen ( FIg. 24 ): OVERAll BlACK, BASAl SCAlE HAIRS BlACK, EXTENDED TO POSTERIOR OF SEgMENT IV, TERgITES VElVETy BlACK, 3 TO 4X AS WIDE AS lONg, TERgITES II –IV WITH lONg HAIRS lATERAlly, REMAINDER WITH MORE EVENly ARRAyED VESTITURE; PlEURITES POORly DEVElOPED; STERNITES PRESENT, BUT SMAll. Genitalia ( FIgS. 25–29 ): HEAVIly PIgMENTED; gONOCOXA BROADER BASAlly THAN lONg, VESTITURE OF SPARSE HAIRS, MICROTRICHIA IN ROWS, HAIRS ACCENTUATED ANTEROlATERAlly, AND MEDIAlly; gONOSTylUS APPROXIMATEly 2.0X lONgER THAN BASAl WIDTH, FlATTENED IN lATERAl VIEW, VESTITURE OF SHORT HAIRS EXCEPT POSTEROlATERAl lONgER gROUPINg, TWO SUBSTANTIAl SHORT, BlUNT, APICAl SPINES ( FIg. 26 ); VENTRAl PlATE ( FIgS. 27–29 ) COMPlEX, 1.6X WIDER THAN lENgTH, POSTERIOR EDgE RAISED AND ROUNDED, BROADly ROUNDED lATERAlly, BROADly CONCAVE ANTEROMEDIAlly; VESTITURE OF COARSE HAIRS; MARKED CENTRAl CARINA, BROADly CROSS-SHAPED APICAlly IN VENTRAl VIEW ( FIg. 25 ); BASAl ARMS NOT MARKEDly DEVElOPED, BUT SUBSTANTIAl, TAPERED SMOOTHly TO JOIN WITH WEll DEVElOPED PARAMERE CONNECTORS; MEDIAN SClERITE WEll DEVElOPED, DISTINCT DISTAl BIFURCATION EXPANDED AND FlARED VENTRAlly ( FIgS. 27, 28 )—NOT VISIBlE IN VENTRAl VIEW; PARAMERES BlADE-lIKE, WITH MARKED ARTICUlATIONS WITH THE VENTRAl PlATE PARAMERE CONNECTORS AND gONOCOXAl APODEME, MEDIAl EDgE WEll DElIMITED, WITH BODy OF PARAMERE TAPERED SMOOTHly TO THIN POSTEROlATERAl EXTREMITy, POSTEROMEDIAlly EXTENDED TO INTERSECT WITH END OF MEDIAN SClERITE BIFURCATIONS AND AggREgATION OF SMAll COARSE SPINES (NOT PART OF THE PARAMERE) MEDIAlly ON THE AEDEAgAl MEMBRANE ( FIg. 29 ); MEMBRANE WITH ARRAyS OF MICROTRICHIA; DORSAl SClERITE ABSENT.

Pupa (BASED ON NUMBERS OF EXUVIAE AND PHARATE ADUlTS). Body: lENgTH, FEMAlE 3.6–4.0 MM ( FIg. 30 ), MAlE 3.5–3.6 MM ( FIg. 31 ), DARK BROWN, SHINy. Head: FRONS OF FEMAlE BlUNTly SHAPED WITH RATIO OF FRONS WIDTH TO VERTEX WIDTH 1.0:1.2 ( FIg. 34 ), THAT OF MAlE MORE ROUNDED; RATIO 1.0:1.9 ( FIg. 35 ); BOTH lACKINg CEPHAlIC DEPRESSION; APPARENTly SMOOTH, BUT COVERED WITH BAREly VISIBlE MINUTE TUBERClES; MUSClE SCARS POSITIVE; FRONTAl SETAE ABSENT, FACIAl SETAE PRESENT, EPICRANIAl SETAE PRESENT, BUT NOT OBVIOUS (OBSCURED By ANTENNAl SHEATHS), IN MAlE WEll lATERAl; POSTORBITAl SPINE ABSENT, BUT ClEAR AREA ON OCUlAR SHIElD IN THAT POSITION; ANTENNAl SHEATH OF FEMAlE EXTENDED TO EDgE OF OCUlAR SHIElD, THAT OF MAlE NOT SO (cf. FIgS. 33 , 34 ). Thorax: ANTERIOR DORSAl SHIElD WITH MINUTE DENSEly PACKED TUBERClES, MORE DISTINCT AND lARgER POSTEROVENTRAl OF gIll BASE ( FIg. 37 ). Gill ( FIg. 33 ): 15 OR 16 lONg THIN FIlAMENTS ARISINg FROM 5 OR 6 SHORT BASAl TRUNKS, SOME BIFURCATINg NEAR BASE, OTHERS MORE DISTAlly; TOTAl lENgTH 2.5–2.7 MM; SHORTER THAN PUPA. SURFACE FINEly ANNUlATED, UNREMARKABlE ( FIg. 33 ; INSERT). Abdomen ( FIg. 36 ): OVERAll, CUTIClE COVERED WITH MINUTE TUBERClES; ARMATURE MINIMAl; TERgITE II lACKINg STIFF SHARP HAIRS; TERgITE III SIMIlAR; TERgITE IV WITH FOUR POSTERIOR STIFF SHARP HAIRS DIRECTED ANTERIORly, TWO FINE HAIRS lATERAlly, TERgITE EXTENDED lATERAlly TO INCORPORATE MUCH OF THE PlEURAl REgION AND ONE FINE HAIR; TERgITE V WITH FOUR HAIRS WITH SPINE COMB SPARSE; TERgITE VI SIMIlAR, BUT WITH THREE HAIRS PER SIDE; TERgITE VII WITH A POSTERIOR PAIR OF FINE HAIRS ON EACH SIDE AND POORly DEVElOPED SPINE COMB ANTERIORly; TERgITE VIII WITH SMAll COMB OF FINE SPINES ANTERIORly AND TWO FINE HAIRS POSTERIORly ON EACH SIDE; TWO TERMINAl SPINES ON TERgITE IX NOT MARKEDly DEVElOPED, BUT SUBSTANTIAl AND SHARPly TAPERED, NOT MARKEDly CURVED, gRAPNEl HOOKS DIFFICUlT TO SEE, A SINglE HAIR AND MINUTE SPICUlES ON EITHER SIDE ( FIg. 38 ); STERNITES III & IV lACKINg HOOK, REPlACED By HOMOlOg HAIR, OTHER HAIR AND SPICUlES AS BEFORE: PlEURITES V – VII AS SMAll PlATES WITH FINE HAIRS; STERNITE V CONTINUOUS MEDIAlly WITH HOOK, HAIR AND SPINUlES AS BEFORE; STERNITES VI AND VII SEPARATED MEDIAlly , EACH PlATE WITH A POSTERIOR SINglE HOOK DIRECTED ANTERIORly, ARRAy OF MINUTE SPICUlES ANTERIORly AND SINglE SMAll HAIR ANTEROlATERAlly; STERNITE VIII AS A SINglE PlATE WITH MEDIAN JUNCTION, WITH ARRAy OF MINUTE SPICUlES ANTERIORly AND A SINglE HAIR ON EACH SIDE; STERNITE IX BElOW THE TERMINAl SPINES FORMED OF TWO lOBES , UPPER ONE WITH TWO SINglE HAIRS AND THE gRAPNEl HOOKS ON EITHER SIDE, THE OTHER lOBE ESSENTIAlly DEVOID OF ARMATURE. Cocoon ( FIgS. 30, 31 ). POORly WOVEN SHAPElESS BAg COVERINg POSTERIOR OF ABDOMEN, OCCASIONAlly REACHINg THORAX; SIlK FIBERS THICK; EXTRANEOUS MATERIAl FROM SUBSTRATE OFTEN INCORPORATED ( FIg. 32 ).

Larva (BASED ON NUMEROUS lAST INSTAR lARVAE). Body ( FIg. 39 ): TOTAl lENgTH 6.4–6.6 MM, EVENly yEllOWISH gREy. Head ( FIg. 40 ): EVENly MEDIUM BROWN WITH DARKER REgIONS; ANTERIOR APOTOME PAlER AND AlONg ECDySIAl lINE TO POSTERIOR OF HEAD; lENgTH 1.18–1.23 MM, WIDTH 0.86–0.88 MM; DISTANCE BETWEEN ANTENNAl BASES 0.55–0.57 MM; ANTERIOR MARgINS OF HEAD PARAllEl, CONVEX POSTERIOR OF STEMMATA; HEAD SPOT PATTERN MARKEDly POSITIVE; ECDySIAl lINES WEll VISIBlE, DIVERgENT AND STRAIgHT (OR SlIgHTly CONCAVE) UNTIl NEAR POSTERIOR OF HEAD, THEN MARKEDly BENT; CERVICAl SClERITES NOT FUSED TO POSTOCCIPUT; gENAE ANTERIOR OF STEMMATA MARKEDly DARK BROWN. Antenna ( FIg. 41 ): NOT EXTENDED BEyOND lABRAl FAN STEM; TOTAl lENgTH 0.48 MM; BASAl AND MEDIAl ANTENNOMERE DARK BROWN, APICAl ANTENNOMERE PAlER (DIAMETER 0.007 MM); BASAl (DIAMETER 0.04 MM) AND MEDIAl (DIAMETER 0.023 MM) ANTENNOMERES SUBEQUAl IN lENgTH, TOgETHER SUBEQUAl TO APICAl ANTENNOMERE; PROPORTIONAl lENgTHS OF THE THREE ANTENNOMERES 1.0:0.8:1.7; PROXIMAl HAlF OF BASAl ANTENNOMERE WITH THICKENED WAll, DISTAl ANTENNOMERE WITH ClOSEly PACKED BANDS ( FIg. 41 ; INSERT). Labral fan: STAlK lIgHT BROWN, ca. 66 RAyS, 7 POSTERIOR RAyS FINER THAN REMAINDER, lENgTH 0.65 MM, MID-RAy WIDTH 0.014 MM; DISTINCT PATTERN OF MICROTRICHIA, lONgER MICROTRICHIA INTERSPERSED WITH 9 OR 10 MARKEDly SMAllER MICROTRICHIA. Mandible ( FIg. 42, 43 ): OVERAll DARKly PIgMENTED; OUTER TEETH SHORT, BROAD, POORly DEVElOPED; APICAl TOOTH ElONgATE AND SUBSTANTIAl; PREAPICAl TEETH, 2 SMAll AND EQUAl IN lENgTH, PROXIMAl TOOTH WEll DEVElOPED; ca. 8 SPINOUS TEETH, SOMETIMES APPARENTly WORN; SENSIllUM AND SERRATION SIMPlE, WEll DEVElOPED ON RAISED BASE; BlADE REgION WITH 6 OR 7 IRREgUlAR SMAll SERRATIONS SOMETIMES APPARENTly WORN. Maxilla ( FIg. 44 ): PAlPUS 2.7X AS lONg AS WIDE, VERy DARK, lONgER THAN MAXIllARy lOBE; DISTINCT gAP BETWEEN lOBE AND PAlP; DENSE TUFT OF HAIR AT BASE OF PAlP. Postgenal cleft ( FIg. 45 ): POORly DEVElOPED, SHAllOW V-SHAPED, WEll SClEROTIZED WITH MARKEDly IRREgUlAR EDgES; POSTERIOR TENTORIAl PITS SMAll; POSTgENAl BRIDgE EVENly DARK BROWN, CONCOlOUROUS WITH gENAE; ElONgATED POSTEROVENTRAl MUSClES SPOTS SMAll. RATIO OF HyPOSTOMA , POSTgENA AND ClEFT 1.0:1.5:0.5. Hypostoma : DARKly PIgMENTED; TEETH IN THREE DISTINCT UNITS, VARIABlE, (SERRA ROAD, FIg. 45 ; INSERT)—TOOTH 0 (MEDIAN) OFTEN WORN AND ROUNDED APICAlly, TOOTH 1 (FIRST SUBlATERAl) MARKEDly SMAllER AND ON BASE OF TOOTH 0, TOOTH 2 MINUTE AND OFTEN NOT EXPRESSED ON ONE SIDE, TOOTH 3 WEll EXPRESSED AND ON BASE OF TOOTH 4 (lATERAl), THAT MARKEDly EXTENDED BEyOND TOOTH 0, TOOTH 5 (PARAlATERAl) BAREly EXPRESSED ON SIDE OF TOOTH 4, TOOTH 6 SMAll BUT DISTINCT ON BASE OF TOOTH 4, TOOTH 7 EXPRESSED MEREly AS lATERAl BUMP, TOOTH 8 ABSENT, All WEll RAISED FROM CURVED BASE OF HyPOSTOMA ; IRREgUlAR lATERAl SERRATIONS; (ZUMSTEIN TRACK, FIg. 46 )—TOOTH 0 (MEDIAN) WEll EXTENDED TO HAlF lENgTH OF TOOTH 4 (lATERAl), TOOTH 1 (FIRST SUBlATERAl) WEll EXPRESSED AND FlANgE-lIKE ON BASE OF TOOTH 0, WITH TOOTH 2 MINUTE AND VARIABlE, OFTEN NOT EXPRESSED ON ONE SIDE, TOOTH 3 lARgER AND INSERTED ON BASE OF TOOTH 4, TOOTH 5 POORly EXPRESSED ON BASE OF TOOTH 4, TOOTH 6 DISTINCT, TOOTH 7 POORly EXPRESSED AS MERE BUlgE, TOOTH 8 ABSENT, All AS STEEP-SIDED UNIT ABOVE REMAINDER OF HyPOSTOMA ; lATERAl SERRATIONS NUMEROUS BUT SMAll, EXTENDED WEll AlONg SIDE OF CONVEX HyPOSTOMA ; 2 OR 3 HyPOSTOMAl SETAE ON EACH SIDE; 3 OR 4 OTHER SMAll SETAE RANDOMly ARRAyED MORE BASAlly. Thorax ( FIg. 47 ): PROTHORAX DARK BROWN, REMAINDER OF THORAX lIgHTER; THE MATURE PUPAl gIll HISTOBlAST POORly L-SHAPED, FIlAMENTS DIRECTED VENTRAlly THEN BROADly POSTERIORly TO RECURVED DORSAlly THEN PARTIAlly ANTERIORly AND THEN DORSAlly, RECURVED TERMINAlly AT gIll BASE; 3 OR 4 BASAl THICKER FIlAMENTS SHOWINg AND SOME BIFURCATIONS. Anterior proleg ( FIg. 48 ): WITH DISTINCT D-SHAPED lATERAl PlATES. Abdomen: EVENly MOTTlED MEDIUM yEllOWISH BROWN. Ventral tubercles: ABSENT OR POORly EXPRESSED. Rectal papillae: THREE SIMPlE lOBES. Anal sclerite ( FIg. 49 ): SIMPlE X-SHAPE, ARMS SUBEQUAl IN lENgTH; ANTERIOR ARMS NOT MARKEDly FlARED APICAlly, MEDIAl REgION WEll DEVElOPED, INTERARM STRUTS ABSENT, POSTEROVENTRAl ARMS SUBSTANTIAl, BUT TAPERED RAPIDly. Posterior circlet: ca. 63 ROWS OF HOOKS WITH 12 OR 13 HOOKS PER ROW (TOTAl ca. 780).

Etymology. NOT SPECIFIED By TONNOIR (1925), BUT NAMED FOR EUSTACE WIllIAM FERgUSON, WEll KNOWN AUSTRAlIAN ENTOMOlOgIST AND PRESIDENT OF THE LINNEAN AND THE ROyAl ZOOlOgICAl SOCIETIES OF NEW SOUTH WAlES, AND WHO COllECTED THE TyPE MATERIAl. FERgUSON WAS NOTED FOR HIS EXPERTISE IN MEDICAl ENTOMOlOgy AND STARTlINg ABIlITIES AS A COllECTOR IN THE FIElD ( VAllEE 2012).

Types. Holotype. MACKERRAS & MACKERRAS (1949: 286) COMMENTED THAT WHEREABOUTS OF THE TyPE OF fergusoni WAS NOT KNOWN, BUT THAT PARATyPES WERE IN THE SCHOOl OF PUBlIC HEAlTH AND TROPICAl MEDICINE, SyDNEy. THE PARATyPES ARE NOW IN ANIC, CSIRO, CANBERRA. BUglEDICH (1999) TOO NOTED THE HOlOTyPE WAS MISSINg. IT IS, HOWEVER, lISTED IN DANIElS (1978: 416) AS IN THE AUSTRAlIAN NATIONAl MUSEUM, SyDNEy. DETAIlS AND IMAgES (e.g. FIgS. 1, 2 ) ARE AVAIlABlE ON THE "ATlAS OF LIVINg AUSTRAlIA" <HTTPS://BIE.AlA.ORg.AU/SPECIES/ URN:lSID:BIODIVERSITy.ORg.AU:AFD.TAXON:ED160F9B-A369-4D87-9EDD-F5B1B298C009> (ACCESSED 4 MAy 2018). LABEl DATA ( FIg. 2 ):- [BUMBERRy NSW./ BITINg MAN/ 1/10/16] [S./ FERgUSONI/ TONN./ DET. A. TONNOIR 1923] [TYPE] [ K48711 ], IN TONNOIR’S HANDWRITINg. THE SPECIMEN IS IN gOOD CONDITION, AlBEIT IT APPEARS THAT THE lEFT MIDDlE lEg IS ABSENT, OTHERWISE THE REMAINDER OF THE BODy IS WEll PRESERVED. NOT EXAMINED BEyOND SUPPlIED IMAgES.

Paratypes. OF THE ElEVEN PINNED FEMAlE PARATyPES DESIgNATED By TONNOIR (1925: 239), BUglEDICH (1999: 328) FOUND 10 IN ANIC. AS OF 2014, (DAC PERS. OBS.) SEVEN WERE AVAIlABlE AND THREE OF THOSE WERE EXAMINED IN DETAIl. LABEl DATA AS FOR HOlOTyPE, BUT WITH:- [ANIC DATABASE NO./ 29 029396]—NOW MOUNTED ON SlIDE. AS ABOVE, BUT WITH:- [ANIC DATABASE NO./ 29 029394] [AUST. NAT./ INS. COll]—NOW ClEARED AND PARTS IN gENITAlIC VIAl ON THE PIN. AS ABOVE, BUT WITH [ANIC DATABASE NO./ 29 029398]—PINNED.

BASED ON lABEl DATA (cf. FIg. 2 & 4 ) AND SIMIlARITy OF HANDWRITINg ON PARATyPES AND HOlOTyPE lABElS, All ARE THOSE DESCRIBED By TONNOIR (1925: 258).

Additional material. APART FROM THE PARATyPES NOTED ABOVE, lARgE NUMBERS OF FINAl INSTAR lARVAE, PUPAE, PUPAl EXUVIAE, REARED AND NETTED FEMAlES, AND REARED MAlES, FROM GRAMPIANS NATIONAl PARK, NAMEly SERRA ROAD, PlUS ZUMSTEIN TRACK AND PICNIC AREA AT MACKENZIE FAllS WERE AVAIlABlE FOR STUDy. REARINg AllOWED FIRM ASSOCIATION OF THE EARlIER PINNED FEMAlES OF TONNOIR TO THE NEWER ADUlTS AND IMMATURE STAgES. THERE ARE SEVEN PINNED FEMAlES FROM COlO VAlE IN ANIC; WE EXAMINED TWO. LABEl DATA [COlO VAlE/ 3. 3. 56 / STATION NO 2. MAN/ E. O’SUllIVAN] [ PARACNEPHIA / FERgUSONI/ (TONNOIR)] [COlO VAlE/ 6. 3. 56 / STATION 4. TO MAN/ 1715-1745 J. CITOWISCH] [ PARACNEPHIA / FERgUSONI/ (TONNOIR)].

REPRESENTATIVE MATERIAl IN ETOH FROM THE GRAMPIANS NATIONAl PARK IS DEPOSITED IN THE ANIC, (ANIC DATABASE NO. 29 026650). SIMIlAR ETOH SAMPlES (UASM# 353360–353362) AND SlIDE MOUNTS (UASM# 353363, 353364, 353146–353150, 353211–353225, 353463–353470), ARE DEPOSITED IN THE STRICKlAND MUSEUM, UNIVERSITy OF AlBERTA <WWW.BIOlOgy.MUSEUMS.UAlBERTA.CA/EN/EHSTRICKlANDENTOMOlOgICAlMUSEUM.ASPX>.

Distribution ( FIg. 84 ). New South Wales: BUMBERRy, S33.1630° E148.5240°, ElEV. 600M., 1-X-1916 ( TONNOIR 1925); ENgINEERS CASCADE, MOUNT VICTORIA, S33.5985° E150.2512°, ElEV. 1043M., JANUARy (WHARTON 1948); COlO VAlE, S34.3779° E150.4462°, ElEV. 700M., MARCH (LEE et al. 1957).

Victoria: COHUNA, S35.8100° E144.2200°, ElEV. 88M. , SEPTEMBER ( MACKERRAS & MACKERRAS 1955); GRAMPIANS NATIONAl PARK, SERRA ROAD, S37.2966° E142.4371°, ElEV. 270M., 8-IX-2011, DAC; ZUMSTEIN PICNIC AREA, S37.0929° E142.3851°, ElEV. 250M., 28-IX-1996, JKM; ZUMSTEIN TRACK, S37.0946° E142.3821°, ElEV. 250M., 12-IX-2014, DAC, JKM, DCC; MACKENZIE FAllS PICNIC AREA, S37.1096° E142.4124°, ElEV. 435M., 13-IX-2014, JKM. THERE IS A PROBABlE ERROR WITH THE lOCAlITy OF “TURRET FAllS, MARySVIllE, NSW” IN MACKERRAS & MACKERRAS (1955: 105). THERE IS NO SUCH NAMED WATERFAll IN THE AREA, BUT RATHER A STEAVENSON FAllS (S37.5327° E145.7737°, ElEV. 630M). A TURRET FAllS (S37.1584° E142.4979°, ElEV. 540M.) IS NEAR HAllS GAP, GRAMPIANS NATIONAl PARK, VICTORIA. THIS IS A MUCH MORE lIKEly lOCAlITy SINCE THERE ARE THIN FIlMS OF WATER AND FlOW IS INTERMITTENT (DAC PERS. OBS., 2014). WE ARE OF THE OPINION THAT THE lOCATION AT MARySVIllE IS IN ERROR.

South Australia: LUCINDAlE, S36.9700° E140.3700°, ElEV. 30M. ( MACKERRAS & MACKERRAS 1949 : 386).

Bionomics. TONNOIR (1925: 239) NOTED THAT TyPE MATERIAl OF FEMAlES (e.g. FIg. 1 ) HAD BEEN COllECTED WHIlE FlyINg AROUND AND BITINg PEOPlE ( FIgS. 2, 4 ), IN ASSOCIATION WITH Austrosimulium furiosum (SKUSE). LABEl DATA ON MATERIAl FROM LUCINDAlE, SOUTH AUSTRAlIA, INDICATES THAT N. ferguson I SERIOUSly INJURED HORSES AND CATTlE, INDEED, MACKERRAS & MACKERRAS (1949: 386) NOTED THAT THIS WAS PROBABly THE BASIS OF THE EARly REPORT By LEA (1917) REgARDINg INJURIOUS SIMUlIIDS.

BITINg BEHAVIOUR WOUlD BE IN KEEPINg WITH THE WEll-DEVElOPED MANDIBlES AND THE ENlARgED SENSORy VESIClE ON THE MAXIllARy PAlPOMERE III, gENERAlly ASSUMED TO BE A CARBON DIOXIDE RECEPTOR ( MCIVER & CHARlTON 1970). SMAllER ABDOMINAl TERgITES ( FIg. 15 ) POINT, AS WEll, TO BlOOD-FEEDINg BEHAVIOR (e.g., ADlER et al. 2004; CRAIg et al. 2012). NUMBERS OF FEMAlE ADUlTS WERE COllECTED FlyINg AROUND THE AUTHORS AT THE PICNIC AREA AT MACKENZIE FAllS, GRAMPIANS NATIONAl PARK, SEPTEMBER, 2014; NONE lANDED OR ATTEMPTED TO FEED.

LARVAE, PUPAE AND REARED ADUlTS ARE FROM THREE lOCAlITIES IN THE GRAMPIANS NATIONAl PARK. JKM COllECTED MATERIAl FROM NEAR ZUMSTEIN PICNIC AREA (S37.0929° E142.3852°, ElEV. 250M.) IN SEPTEMBER, 1996. ON 8TH SEPTEMBER, 2011, DAC COllECTED FROM THE DOWNSTREAM SIDE OF A CUlVERT ON SERRA ROAD (S37.2966° E142.4371°, ElEV. 270M.) ( FIg. 50 ). LARgE NUMBERS OF lARVAE AND PUPAE WERE ON THE lIP OF THE CUlVERT PIPE AND ON HARD SURFACES A FEW METRES DOWNSTREAM. LARVAE FORMED TyPICAl SPACED ARRAyS ( FIg. 51 ). AT 13:00 HRS, AIR TEMPERATURE WAS 14.1 °C, WATER 11.0°C, CONDUCTIVITy 120µS/CM, PH 7.7, VElOCITy ca. 0.62 M/S AND THE WATER SlIgHTly TURBID . LARVAE WERE MAINly IN SHAllOW EVEN FlOW AND NOT FOUND ON STONES OR VEgETATION WHERE FlOW WAS MARKEDly TURBUlENT. THIS SITE WAS DRy IN SEPTEMBER, 2014, AND ClEARly HAD NOT HAD RUNNINg WATER SINCE 2011, A yEAR WITH SERIOUS FlOODINg IN THE GRAMPIANS.

A THIRD SITE WAS A SMAll STREAM ON ZUMSTEIN SHORTCUT (S37.0946° E142.3821°, ElEV. 250M.) ( FIg. 52 ). ON 12 SEPTEMBER 2014, TEMPERATURES WERE—AIR 17.5°C, WATER 16.6°C, VElOCITy 0.7 M/S IN THE SMAll RIFFlES. LARVAE WERE ON TOP AND UNDER STONES WITH PUPAE UNDER STONES ( FIg. 32 ). FEMAlE ADUlTS WERE NETTED AND BOTH SEXES REARED FROM PUPAE. OF NOTE IS THAT THE AREA HAD BEEN INTENSEly BURNED SOME MONTHS BEFORE THE COllECTION AND CONSIDERABlE ASH WAS DRIFTINg IN THE WATER. THIS STREAM WAS REVISITED IN NOVEMBER, 2014 AND WHIlE EXPECTED TO BE DRy, THERE WAS A SMAll BASE FlOW. THERE WAS NO EVIDENCE THAT N. fergusoni HAD EVER BEEN PRESENT. THE ONly SIMUlIID PRESENT WAS Simulium ornatipes SKUSE, WITH lARgE NUMBERS OF lARVAE AND PUPAE.

OF NOTE FOR THE SEPTEMBER COllECTION OF N. fergusoni PUPAE FROM ZUMSTEIN SHORTCUT WAS THAT THE COCOONS WERE HEAVIly COVERED WITH SMAll STONE PARTIClES FROM THE SUBSTRATE ( FIg. 32 ). SUCH WAS NOT SEEN AT THE SERRA ROAD CUlVERT SITE. WITH SUCH SUBSTRATE lACKINg, COCOONS THERE HAD ONly AlgAl STRANDS INCORPORATED INTO THE RUDIMENTARy COCOON.

OUR DATES OF COllECTINg ARE IN gOOD AgREEMENT WITH TONNOIR'S ORIgINAl SAMPlE OF N. fergusoni FEMAlES TAKEN 1st OCTOBER, 1916. MACKERRAS & MACKERRAS (1952: 105) COMMENTED THAT THEy HAD SEARCHED EXTENSIVEly FOR IMMATURES OF N. fergusoni AT BUMBERRy, NSW, BUT WERE UNSUCCESSFUl.

THE THREE GRAMPIAN lOCAlITIES gIVE ClEAR EVIDENCE THAT N. fergusoni IS A UNIVOlTINE AUSTRAl SPRINg SPECIES THAT INHABITS INTERMITTENT STREAMS. THE WIDE RANgINg FEMAlES, AS NETTED AT THE MCKENZIE FAllS PICNIC AREA, ARE PROBABly lOOKINg FOR A BlOOD MEAl AND/OR SUITABlE SITES TO lAy EggS. THERE IS, THOUgH, NO INFORMATION ON OVIPOSITION.

THE COlO VAlE SPECIMENS WERE TAKEN DURINg A PRElIMINARy INVESTIgATION INTO CONTROl OF RABBITS WITH THE MyXOMA VIRUS (LEE et al. 1957). THE ADUlTS WERE COllECTED IN MARCH—AT CONSIDERABlE VARIANCE TO THE COllECTION DATES FROM THE GRAMPIANS. HOWEVER, ElEVATION AT THE COlO VAlE lOCAlITy IS SOME 700M., SO COlD WATER WOUlD OCCUR lATER INTO THE yEAR.

THE lOW NUMBER (ca. 780) OF HOOKS ( FIg. 49 ) COMPRISINg THE CIRClET OF HOOKS IS IN gOOD AgREEMENT WITH THE VElOCITIES RECORDED (~0.62 M/SEC.). PAlMER & CRAIg (2000), CRAIg et al. (2012), KONURBAEV (1973) AND OTHERS, HAVE SHOWN ClOSE CORRElATION BETWEEN NUMBER OF SUCH HOOKS AND gENERAl VElOCITy OF FlOW IN THE HABITAT.

BUglEDICH (1999) INDICATED THAT HUNTER & MOOREHOUSE (1976) DEAlT WITH BIOlOgy OF N. fergusoni , BUT THIS IS IN ERROR.

Remarks. IN THE SyNONyMy gIVEN By MACKERRAS & MACKERRAS (1949: 386) FOR Simulium fergusoni , SHARP [sic] (1945: 505) IS IN ERROR AND IT SHOUlD BE 'SMART'.

AS TONNOIR (1925) NOTED, N. fergusoni ADUlT FEMAlES COUlD AT THAT TIME BE SEPARATED FROM OTHER AUSTRAlIAN SIMUlIIDS By COMPlETE lACK OF CAlCIPAlA AND PEDISUlCUS, A SHORT PAlPOMERE IV AND SMAll APICAl ANTENNOMERE. THIS gENERAlly AlSO APPlIES TO N. occidentalis (SEE BElOW). ABSENCE OF A CAlCIPAlA WAS THEN UNIQUE AMONg AUSTRAlIAN SIMUlIIDS, BUT EXAMPlES OF A SMAll OR ABSENT CAlCIPAlA ARE NOW KNOWN (CRAIg et al. 2018) FOR WESTERN AUSTRAlIA Bunyipellum gladiator (MOUlTON & ADlER) AND UNDESCRIBED SPECIES RElATED TO EASTERN AUSTRAlIA " Paracnephia umbratora " (TONNOIR).

THE FUNCTION OF THE CAlCIPAlA IN SIMUlIID ADUlTS REMAINS UNClEAR, AlTHOUgH IT IS BElIEVED TO PlAy A ROlE IN gROOMINg THE WINg OR THE BODy (CROSSKEy 1990: 345; ADlER et al. 2004: 41). THE ROW OF STOUT SPINES AlONg THE VENTRAl SURFACE OF THE HIND BASITARSUS IS DISTINCT IN N. fergusoni AND, IN PARTICUlAR, THERE IS A CONCENTRATION, OR ClUMP, OF SPINES WHERE THE BASE OF A CAlCIPAlA WOUlD OCCUR ( FIg. 13 ). THIS WAS IllUSTRATED By TONNOIR (1925: 221; HIS FIg. 2 F, G, H ), INClUDINg THE ElONgATED TARSOMERE II—UNUSUAlly lONg FOR AUSTRAlIAN SIMUlIIDS. SUCH AN AggREgATION OF SPINES AlSO OCCURS IN N. occidentalis ON A VERy SMAll CAlCIPAlA. THAT CONDITION IN N. occidentalis IS NOT UNIQUE WITHIN AUSTRAlIAN SIMUlIIDS, BEINg PRESENT IN Bunyipellum gladiator (MOUlTON & ADlER) (CRAIg et al. 2018) AND AS yET UNDESCRIBED AUSTRAlIAN SPECIES RElATED TO Paracnephia umbratora . AggREgATION OF SPINES SUggESTS THAT THEy PERHAPS PERFORM THE FUNCTION OF THE CAlCIPAlA WHEN THE lATTER IS SMAll OR ABSENT.

THE PUPAE OF BOTH SPECIES ARE SIMIlAR, WITH gIllS OF N. occidentalis lONgER THAN THOSE OF N. fergusoni . LARVAE OF N. fergusoni ARE DARKER IN COlOUR THAN N. occidentalis AND THE lATTER HAS lARgER lABRAl FANS. EXPRESSION OF lARVAl HyPOSTOMAl TEETH IN Nothogreniera IS UNIQUE FOR AUSTRAlIAN SIMUlIIDS. THE MARKEDly TRIlOBED ARRANgEMENT OF TEETH (e.g., FIg. 46 ) IS SIMIlAR TO THOSE IN Greniera AND Tlalocomyia (ADlER et al. 2004: e.g. 564). WHIlE TRIlOBED ARRANgEMENTS ARE KNOWN FOR lARVAE OF " Paracnephia " fuscoflava " (MACKERRAS & MACKERRAS) AND " P. " tonnoiri (DRUMMOND), THE lOBES ARE NOT AS STRONgly DEVElOPED.

THERE ARE DISCREPANCIES BETWEEN THE TyPE ( FIg. 1 ) AND NEWER MATERIAl FROM THE GRAMPIANS ( FIg. 3 ); NAMEly, THE DARKER COlOURATION OF THE TyPE AND DENSITy OF THE VESTITURE ON THE SCUTUM. THESE AND OTHER DIFFERENCES SUggEST THAT N. fergusoni IS A SPECIES COMPlEX. UNFORTUNATEly, EFFORTS By MACKERRAS & MACKERRAS (1952: 105) TO COllECT IMMATURE STAgES FROM THE TyPE lOCAlITy (BUMBERRy, VICTORIA) PROVED UNSUCCESSFUl. INDICATION OF A SPECIES COMPlEX IS FURTHER SUggESTED By DIFFERENCES IN THE HyPOSTOMAl TEETH OF lAST INSTAR lARVAE BETWEEN TWO POPUlATIONS FROM THE GRAMPIANS; NAMEly SERRA ROAD AND ZUMSTEIN TRACK (cf. FIgS. 45, 46 ). THE lATTER HAS A HyPOSTOMA THAT IS MARKEDly SIMIlAR IN APPEARANCE TO THOSE OF Greniera (ADlER et al. 2004: 563). GIVEN THAT THE DISTANCE BETWEEN THE TWO SITES IS A MERE 24 KM, PERHAPS THE FEMAlE ADUlTS ARE NOT AS VAgIlE AS WE SUggEST ABOVE. OR, COUlD SUCH DIFFERENCES BE ENVIRONMENTAlly DICTATED?

THE UNIDENTIFIED lARVAE REPORTED AND IllUSTRATED By WHARTON (1949: 411, HIS FIgS 17 -21) FROM ENgINEERS CASCADE, MOUNT VICTORIA, NSW, ARE REFERABlE TO N. fergusoni , AS HERE DEFINED, DESPITE MINOR DISCREPANCIES (e.g., PUPAl gIll) FROM THE MATERIAl REPORTED HERE . FURTHER STUDIES INClUDINg CyTOlOgICAl AND MOlECUlAR DATA ARE NEEDED TO ASCERTAIN THE STATUS OF THESE WIDEly DISPERSED POPUlATIONS.

Figures

FIGURE 84. Map of southern part of Australia, showing distribution of Nothogreniera.

FIGURES 1–6. Nothogreniera fergusoni adult female. (1) Habitus of holotype (Bumberry, 1916). Scale bar = 1.0 mm. (Image courtesy of Russell Cox, Australian Museum, Sydney). (2) Holotype labels. (Image courtesy of Melissa Graf, Australian Museum, Sydney). (3) Habitus of female. In ethanol. (Grampians National Park. 2014). Scale bar = 1.0 mm. (4) Paratype labels. (Australian National Insect Collection, Canberra). (5) Frontal view of head. (Grampians National Park). Distal palpomere foreshortened. Scale bar = 0.2 mm. (6) Antenna, paratype (ANIC No. 29 029396. Bumberry). Scale bar = 0.2 mm.

FIGURES 7–11. Nothogreniera fergusoni adult female. (7) Maxillary palpus, lacinia. Scale bar = 0.2 mm. Insert, lacinia tip. Scale bar = 0.05 mm. (8) Mandible tip. Scale bar = 0.05 mm. (9) Cibarium, paratype (ANIC No. 29 029396. Bumberry). Scale bar = 0.1 mm. (10) Anepisternal membrane, paratype. (As for Fig. 9). Scale bar = 0.1 mm. (11) Wing, paratype. (As for Fig. 9). Scale bar = 0.5 mm.

FIGURES 12–17. Nothogreniera fergusoni adult female. (12) Higher magnification of costal vein showing hair-like setae. Scale bar = 0.1 mm. (13) Hind basitarsus. Arrow points to concentration of spines at position of absent calcipala. Scale bar = 0.1 mm. (14) Claw. Arrow indicates minute tooth. Scale bar = 0.02 mm. (15) Abdominal tergites. Scale bar = 0.5 mm. (16) Pharate spermatheca, showing internal acanthae and sculpted junction of spermathecal duct. Stained with Chlorazol Black. Scale bar = 0.02 mm. (17) Spermatheca, fully developed, paratype. (As for Fig. 9). Scale bar = 0.05 mm.

FIGURES 18–20. Nothogreniera fergusoni, adult female. FIGURES 21–23. Male. (18) Genital fork, paratype (As for Fig. 9). Scale bar = 0.05 mm. (19) Hypogynial valves, paratype (as for Fig. 9). Scale bar = 0.05 mm. (20) Anal lobe and cercus, paratype (as for Fig. 9). Scale bar = 0.05 mm. (21) Frontal view, head, reared (Grampians National Park). Scale bar = 0.25 mm. (22) Antenna. Scale bar = 0.1 mm. (23) Maxillary palpus. Scale bar = 0.1 mm.

FIGURES 24–29. Nothogreniera fergusoni, male. (24) Abdominal tergites, reared. Scale bar = 0.5 mm. (25) Ventral view, genitalia, reared. gc—gonocoxa; gs—gonostylus; p—paramere; v p—ventral plate. Scale bar = 0.1 mm. (26) Gonostylus—gs & gonocoxa—gc. Scale bar = 0.05 mm. (27) Ventral plate. Posterior view. m s—median sclerite. Scale bar = 0.05 mm. (28) Ventral plate, lateral view. b a—basal arm; m s—median sclerite; p c—paramere connection. Scale bar = 0.05 mm. (29) Genitalia, slide mounted. b a—basal arm; gc—gonocoxa; m s—median sclerite; p—paramere; p c—paramere connection; v p—ventral plate. Scale bar = 0.05 mm.

FIGURES 30–33. Nothogreniera fergusoni, pupae. (30) Habitus (female). Scale bar = 1.0 mm. (31) Habitus (male). Scale bar = 1.0 mm. (32) Pupae, in situ. (Zumstein Track Stream, see Fig. 52). (33) Pupal gill. Scale bar = 0.5 mm. Insert: Surface of gill filament. Scale bar = 0.05 mm.

FIGURES 34–38. Nothogreniera fergusoni, pupae (exuviae). (34) Cephalic capsule, female. Scale bar = 0.2 mm. (35) Cephalic capsule, male. Scale bar = 0.2 mm. (36) Abdomen, slide mount. Scale bar = 1.0 mm. (37) Thoracic cuticle. Scale bar = 0.2 mm. (38) Segment IX, showing terminal spines. Arrow indicates grapnel hooks. Scale bar = 0.1 mm.

FIGURES 39–43. Nothogreniera fergusoni, last instar larva. (39) Habitus. Scale bar = 1.0 mm. (40) Dorsal head. Scale bar = 0.5mm. (41) Antenna. Scale bar = 0.05 mm. Insert: high magnification DIC of apical antennal article. Scale bar = 0.02 mm. (42) Mandible of last instar. Scale bar = 0.1 mm (43) Mandible tip. Scale bar = 0.02 mm.

FIGURES 44–49. Nothogreniera fergusoni, last instar larva. (44) Maxilla, palpus. Scale bar = 0.05 mm. (45) Postgenal cleft, hypostoma. (Serra Road). Scale bar = 0.2 mm. Insert. Hypostomal teeth. Scale bar = 0.05 mm. (46) Hypostoma. (Zumstein). Scale bar = 0.1 mm. (47) Lateral view of thorax, showing gill histoblast. Scale bar = 0.2 mm. (48) Anterior proleg. Penultimate instar. Scale bar = 0.05 mm. (49) Anal sclerite & circlet of hooks. Scale bar = 0.2 mm.

FIGURES 50–52. Nothogreniera fergusoni, habitats of immatures. (50) Serra Road, culvert, Grampians National Park, Victoria. (September 2011). (51) Late instar larvae. Arrow indicates direction of flow. (52) Zumstein Track Stream, Grampians National Park, Victoria. (September 2014).