Nesterenkonia sphaerica, Zhang, Gaiyun, Wang, Lina, Xie, Fuquan, Pei, Shengxiang & Jiang, Li, 2020

Zhang, Gaiyun, Wang, Lina, Xie, Fuquan, Pei, Shengxiang & Jiang, Li, 2020, Nesterenkonia salmonea sp. nov. and Nesterenkonia sphaerica sp. nov., isolated from the Southern Atlantic Ocean, International Journal of Systematic and Evolutionary Microbiology 70 (2), pp. 923-928 : 926-927

DESCRIPTION OF NESTERENKONIA SPHAERICA SP. NOV.

Nesterenkonia sphaerica (sphae'ri.ca. L. fem. adj. sphaerica spherical, referring to the cell shape of the type strain).

Cells are Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci (diameter 0.7–0.8 µm) without any flagella. Colonies on MA are approximately 1 mm in diameter, opaque, smooth, circular with regular margins, convex and light-yellow in colour after 6 days at 30 °C. Growth occurs in 0–9% (w/v) NaCl (optimum, 1–4%), at pH 7–12 (pH 9–10) and at 10–40 °C (30–35 °C). Positive for catalase and oxidase. Negative for nitrate reduction, methyl red test, production of H 2 S and melanin, Voges– Proskauer reaction, and hydrolysis of starch, cellulose and casein. Assays using the API ZYM system are positive for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase and naphthol-AS-BI-phosphohydrolase; weakly positive for valine arylamidase and acid phosphatase; negative for N -acetyl- β -glucosaminidase, cystine arylamidase, α -chymotrypsin, α -glucosidase, lipase (C14), trypsin, α -galactosidase, β -glucuronidase, β -galactosidase, β -glucosidase, α -mannosidase and β -fucosidase. Analysis using the API 20NE system reveals that cells are positive for hydrolysis of aesculin and gelatin; negative for the remaining 18 substrates. The major menaquinones are MK-7, MK-8 and MK-9. The peptidoglycan type is A4 α, L-Lys–L-Glu. The polar lipids detected are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid, two unidentified phospholipids and three unidentified lipids. The major fatty acids are anteiso-C 15:0 and anteiso-C 17:0; in addition, significant amounts of iso-C 16:0, iso-C 15:0 and C 16:0 are also present. The genomic DNA G+C content of the type strain is 64.3 mol%.

The type strain, GY239 T =(KCTC 39640 T =MCCC 1A10688 T), was isolated from marine sediment collected from the Southern Atlantic Ocean .