Neocosmospora bomiensis Z.Q. Zeng & W.Y. Zhuang, 2017

Zeng, Zhao-Qing & Zhuang, Wen-Ying, 2017, Two new species of Neocosmospora from China, Phytotaxa 319 (2), pp. 175-183 : 177-178

Neocosmospora bomiensis Z.Q. Zeng & W.Y. Zhuang , sp. nov. FIGURE 2 .

Etymology:—The specific epithet refers to the type locality.

Fungal Names: FN570412.

Mycelium not visible around ascomata or on natural substrata. Ascomata superficial, solitary, non-stromatic or with a tiny basal stroma, subglobose to globose, or pyriform, lateral collapse upon drying, orange-red, turning brownish red in KOH, becoming yellow in LA, 255−363 × 206−204 μm (n = 12). Perithecial surface slightly warted, warts 20−50 μm

high, of textura globose to t. angularis, cells 12−33 × 14−30 μm, walls 1−1.2 μm thick. Perithecial wall of one layer, 20−30 μm thick, of textura angularis to t. prismatica, cells 10−28 × 4−15 μm, walls 1−1.2 μm thick. Asci clavate, 8- spored, 60−100 × 8−15 μm. Ascospores ellipsoidal, 1-septate, hyaline, smooth, biseriate, 10–18(–20) × 5–8 μm.

Colony on PDA growing moderately slow, 32 mm diam after 1 week at 25 °C, surface floccose, aerial mycelium white, producing light violet pigment in medium. Colony on SNA 40 mm diam after 1 week at 25 °C, surface with sparse whitish aerial mycelium. Conidiophores arising from aerial mycelium, branched, 20–63 μm long, 2.5–3.5 μm at base. Macroconidia sickle-shaped, slightly curved, hyaline, (3–)5–6-septate, 5-septate: 53−70 × 4–5 μm, 6-septate: 55−70 × 4–5 μm.

Type: — CHINA. Tibet Autonomous Region, Bomi County, 29°53’26.15”N, 95°43’6.53”E, alt. 2700 m, on twigs, 22 Dec 2016, Z.Q. Zeng, Z.H. Yu, H. D. Zheng, X.C. Wang, K. Chen & Y.B. Zhang 11153 ( HMAS 254519 ! holotype) ; dried ex-type culture, HMAS 248885 .

Notes: —Morphologically N. bomiensis resembles N. rectiphora Samuels, Nalim & Geiser in having solitary, subglobose to globose, orange-red, warted perithecia which are collapsing laterally by pinching when dry, and size of asci and ascospores ( Nalim et al. 2011). However, N. rectiphora differs in having wider asci (9–18 μm vs. 8−15 μm) and broadly ellipsoidal to slightly fusiform, yellow brown and striate rather than ellipsoidal, hyaline and smooth ascospores ( Nalim et al. 2011). Although N. bomiensis is phylogenetically related to N. rectiphora ( Fig. 1 , MPBP / BIPP = 94%/100%), the sequence comparisons reveal that there are 31 bp divergences in the ITS region and 13 bp unmatched loci in the tef1 gene between HMAS 254519 and GJS 0289 (ex-holotype culture of N. rectiphora ).

Figures

FIGURE 1. Phylogenetic tree generated by maximum parsimony analysis inferred from the combined ITS and tef1 partial sequences. The new taxa introduced are in bold. MPBP (left) above 50% and BIPP (right) above 90% are indicated at nodes.

FIGURE 2. Neocosmospora bomiensis (HMAS 254519). a−d. Ascomata on natural substrate. e. Colony on PDA. f. Colony on SNA. g. Section of an ascoma. h. An ascus with ascospores. i−l. Ascospore. m−o. Conidiophores and macroconidia. p−r. Macroconidia. Scale bars: a = 1 mm, b−d = 0.5 mm, g = 50 μm, h−l = 10 μm, m−r= 20 μm.