Crous, Hernández-Restrepo & Groenewald & Crous, 2015

Hernández-Restrepo, Margarita, Groenewald, Johannes Z. & Crous, Pedro W., 2015, Neocordana gen. nov., the causal organism of Cordana leaf spot on banana, Phytotaxa 205 (4), pp. 229-238 : 234-235

Neocordana musae (Zimm.) M. Hern. -Rest. & Crous , comb. nov. MycoBank MB811106, Figure 3.

Basionym : Scolecotrichum musae Zimm., Zentbl. Bakt. ParasitKde, Abt. II 8: 220 (1902) (as Scolicotrichum )= Cordana musae (Zimm.) Höhn., Zentbl. Bakt. ParasitKde, Abt. II 60: 7 (1923)

Mycelium superficial and immersed, hyphae smooth, hyaline to brown; intracellular hyphae smooth, hyaline to brown. Conidiophores brown, smooth, 46–118.5 × 5–6.5 μm. Conidiogenous cells terminal and intercalary, 15–72.5 × 5–9 μm, denticulate; denticles up to 2 μm long, 0.5–1.5 μm wide. Conidia obclavate, pyriform, mostly obovoid, 14.5–19 × 8–11.5 μm, 1–septate, thick-wall, pale brown, base darker, flat, 1–1.5 μm wide.

Culture characteristics:—Colonies on MEA after 1 week attaining 25 mm diam, elevated, cottony, aerial mycelium white with mouse grey zones (sporulation), exudate hyaline to pale luteous, margin diffuse; reverse black in the centre, with concentric rings isabelline to umber or luteous to ochreous. On OA colonies very similar to that observed on MEA

Type: — INDONESIA. Java, Buitenzorg, 1920, on Musa sapientum, Zimmermann A., Centralbl. F. Bakt Abt. II. Bd. 8. S 220 (holotype not found and presumed missing). MALAYSIA. 1 April 2011, on Musa sp. , Mee Hua Wong (neotype designated here CBS H-22054!, ex-neotype strain CPC 18127 = CBS 139318).

Additional cultures examined:— AUSTRALIA. Queensland, 21 January 2011, on Musa sp. , unknown collector, CBS 139319 = CPC 19065. MEXICO. Tabasco, Villa Hermosa, 16 December 2008, on Musa sp. , Maria de J. Yarez Morales, CPC 16362; Tapechula, Chiapas, 16 December 2008, on Musa sp. , Maria de J. Yarez Morales, CPC 16298. UNKNOWN COUNTRY, February 1934, on Musa sapientum, G. Stahel, CBS 151.34.

Distribution:—Widespread in the tropics with the host.

Notes:—In the ITS phylogenetic tree ( Figure 2 ) Neocordana musae is represented by six isolates from Musa leaves collected from different countries, namely Australia, Malaysia and Mexico. Originally N. musae was described from Java ( Zimmermann 1902), but the type material is presumably lost. Among the isolates studied here, we chose isolate CPC 18127 as ex-neotype because Malaysia is closest to the location where this fungus was collected for the first time; furthermore this isolate is morphologically similar to the fungus described and illustrated by Zimmermann (1902), and occurs commonly on Musa spp. in tropical regions.

Figures

FIGURE 2. Maximum likelihood (RAxML) phylogenetic tree based on ITS sequences of Neocordana isolates. Bayesian posterior probability values and Bootstrap support values are shown at the nodes. The tree was rooted with Pyricularia bothriochloae KF777186 and Pyricularia angulata JF719830. Ex-type isolates are indicated in bold.