Myrinia manchada Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 30

Myrinia manchada Grishin , new species

https://zoobank.org/ F341D06C-4741-4C54-ABCE-A7BD83C5C264

( Fig. 2 part, 67–68, 282–284)

Definition and diagnosis. Sequencing of the holotype of Myrinia laddeyi (E. Bell, 1942) (type locality in Ecuador) revealed that it is a species related to Myrinia binoculus (Möschler, 1877) (type locality in Suriname) and not the species Evans (1953) identified as M. laddeyi ( Fig. 2). Therefore, the species that Evans misidentified as M. laddeyi does not have an available name and is new. In USNM, we found a specimen that keys to E. 24.4 in Evans (1953) and is distinguished from its relatives, including the true M. laddeyi , by larger size (forewing typically longer than 21 mm), more segments in nudum (more than 25 segments), the lack of eyespot on the ventral forewing, large dark tornal spot on the ventral hindwing, short and rounded harpe of the right valva, style of the left valva longer than harpe. This species is not cryptic and is diagnosed reliably by phenotype. In DNA, a combination of the following base pairs is diagnostic in the nuclear genome: aly798.33.50:A117G, aly 2149.2.8:G45A, aly38.5.1:T141C, aly767.7.2:T78C, aly767.7.2:T468A, aly651.4.5:T106T (not C), aly536.173.2:C78C (not T), aly17.12.4:C112C (not T), aly17.12.4:A336A (not G), aly 1022.2.1:G96G (not A), and COI barcode: T82C, 277C, T364A, T352C, T386C.

Barcode sequence of the holotype. Sample 11-BOA-13383A10, GenBank OR837652, 658 base pairs: AACTTTATATTTTATTTTTGGAATTTGAGCAGGAATAGTTGGAACATCTTTAAGTTTATTAATTCGAACTGAATTAGGTAACCCAGGATCATTAATT GGAGATGATCAAATTTATAATACTATTGTTACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAATT GACTTGTACCATTAATATTAGGAGCCCCAGATATAGCTTTCCCCCGAATAAATAATATAAGATTTTGATTATTACCCCCCTCCTTAATATTATTAAT CTCCAGAAGAATTGTAGAAAATGGAGCAGGAACTGGATGAACTGTTTATCCCCCTTTATCCGCTAATATTGCACACCAAGGATCTTCAGTAGACCTA GCTATTTTTTCTCTACATTTAGCTGGAATTTCATCTATTTTAGGAGCTATCAATTTTATTACAACAATTATTAATATACGTATTAATAATCTTTCAT TTGATCAAATACCATTATTCGTTTGAGCTGTAGGAATCACAGCTTTATTATTATTATTATCTTTACCTGTATTAGCAGGTGCTATTACTATACTTCT AACAGATCGAAATTTAAATACATCTTTTTTTGATCCTGCTGGAGGAGGAGATCCTATTCTTTATCAACATTTATTT

Type material. Holotype: ♂ deposited in the National Museum of Natural History , Smithsonian Institution, Washington, DC, USA ( USNM), illustrated in Fig. 67–68, bears the following five rectangular labels, four white: [ GUYANA: Region 8 | Potaro River nr. Tukeit | 250′ – 1000′ | 18-23 Mar 1999 | leg. S. Fratello], [DNA sample ID: | 11-BOA-13383A10 | c/o Nick V. Grishin], [DNA sample ID: | NVG-22034H05 | c/o Nick V. Grishin], [{QR Code}| USNM ENT 00232398 ], and one red [HOLOTYPE ♂ | Myrinia | manchada Grishin ] . Paratype: 1♂: NVG-23026F10, EL83681 French Guiana, Saint-Laurent-du-Maroni, 1905, E. Le Moult leg. [ MNHP].

Type locality. Guyana: Potaro-Siparuni Region, Potaro River nr. Tukeit.

Etymology. In Spanish, manchada means stained, spotted, spotty, or smudgy. The name is given for the large tornal spot on the ventral hindwing and is a noun in apposition.

Distribution. The Guianas.