Muyocopron castanopsis Mapook, Boonmee & K.D. Hyde, 2016

Mapook, Ausana, Hyde, Kevin D., Dai, Dong-Qin, Li, Junfu, Gareth Jones, E. B., Bahkali, Ali H. & Boonmee, Saranyaphat, 2016, Muyocopronales, ord. nov., (Dothideomycetes, Ascomycota) and a reappraisal of Muyocopron species from northern Thailand, Phytotaxa 265 (3), pp. 225-237 : 230-232

Muyocopron castanopsis Mapook, Boonmee & K.D. Hyde , sp. nov.

Index Fungorum number: IF551616, Facesoffungi number: FoF 01888; Fig. 2

Etymology: Named after the host genus Castanopsis .

Holotype: MFLU 15–1131

Saprobic on dried twigs of Castanopsis indica (Roxb.) Rehder. Sexual morph: Ascomata (140–)170–250 μm high × 450–750 μm diam. (x = 196.5 × 562 μm, n = 5), superficial, solitary or scattered, coriaceous, appearing as circular, scattered, flattened, brown to dark brown spots, covering the host, without a subiculum, with a poorly developed basal layer and an irregular margin. Ostiole central. Peridium 25–35 μm wide, widest at the sides, outer layer comprising dark brown to black, pseudoparenchymatous, occluded cells of textura angularis, inner layer comprising light brown cells of textura angularis. Hamathecium comprising 1.5–2.5 μm wide, cylindrical to filiform, septate, pseudoparaphyses. Asci (85–)95–110 × 23–24(–28) μm (x = 98 × 24 μm, n = 10), 8-spored, bitunicate, saccate or broadly obpyriform to ovoid, pedicellate, straight or slightly curved, with an ocular chamber. Ascospores 20–26 × 10–13 μm (x = 23 × 11.5 μm, n = 20), overlapping 2–4 seriate, hyaline, ellipsoid to obovoid, with obtuse ends, aseptate, with granular appearance. Asexual morph: Undetermined.

Culture characteristics:— Ascospores germinating on MEA within 24 hr. at room temperature and germ tubes produced from both ends of the ascospores. Colonies on MEA reaching 0.8 cm diam after 1 week, at 25 oC. Aerial mycelium initially white, slightly raised, in old cultures grayish to light brown, flattened on surface, brown to dark brown from below, margin light brown to white.

Material examined:— THAILAND, Chiang Rai, on dried twigs of Castanopsis indica ( Fagaceae ), 22 July 2014, A. Mapook, ( MFLU 15–1131, holotype), ex-type culture MFLUCC 14–1108, BCC; ibid. ( HKAS 92522, isotype); on dried twigs of Castanopsis indica ( Fagaceae ), 26 August 2014, A. Mapook ( MFLU 16–0476, paratype)

Notes:— Muyocopron castanopsis is a saprobe on dried twigs of Castanopsis indica (Roxb.) Rehder. Our collection is most similar to Muyocopron hongkongense in size and shape of the ascospores, but it differs in having larger ascomata ((140–)170–250 × 450–750 μm vs. 220–440 × 92–160 μm) and longer ascospores ( Table 2) and different host families ( Fagaceae vs Arecaceae ). Thus, we consider our collection to be a new species based on morphology.

Figures

FIGURE 2. Muyocopron castanopsis (holotype). a–c. Superficial ascomata on substrate. d–e. Squash mounts showing ascomata walls. f. Section of ascoma. g. Ostiole h. Peridium. i. Pseudoparaphyses. j–m. Asci. n–r. Unicellular ascospores. s. Germination of ascospore. Scale bars: b,c = 500 μm, d = 200 μm, g = 100 μm, j–m = 20 μm, e, h, i, n–s = 10 μm, f = 5 μm.