Microsporomyces ellipsoideus Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 114

Microsporomyces ellipsoideus Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828818. Fig. 15F .

Etymology: the specific epithet ellipsoideus refers to the ellipsoidal vegetative cells of the type strain.

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are ellipsoidal or cylindrical, 6.0–7.5 × 9.0–14.5 μm and single, budding is polar ( Fig. 15F ), a sediment is formed. After 1 mo at 17 °C, a ring and sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is brownish-orange, butyrous, smooth. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, galactose, sucrose, maltose, trehalose, melibiose (weak), raffinose, soluble starch, glycerol, D-glucitol, Methyl-α- D-glucoside, salicin and succinate (delayed and weak) are assimilated as sole carbon sources. L-sorbose, cellobiose, lactose, melezitose, inulin, D-xylose, L-arabinose, D-arabinose, D-ribose, L-rhamnose, D-glucosamine, methanol, ethanol, erythritol, ribitol, galactitol, D-mannitol, DL-lactate, citrate, myo-inositol and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate and L-lysine (weak) are assimilated as sole nitrogen sources. Sodium nitrite, ethylamine hydrochloride and cadaverine dihydrochloride are not assimilated. Maximum growth temperature is 26–27 °C. Growth in vitamin-free medium is negative. Starch-like substances are not produced. Growth on 50 % (w/w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Mi. ellipsoideus differs from its closely related species Mi. rubellus in its inability to assimilate melezitose, ribitol and galactitol and its ability to soluble starch and Methyl-α- Dglucoside ( Table S1.24 ).

Typus: China, Motuo county, Tibet, obtained from a leaf of an unidentified plant, Sep. 2014, Q.-M. Wang (holotype CGMCC 2.5664 T preserved in a metabolically inactive state, ex-type CBS 16020 = XZ137E4).

Figures

Fig. 15. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) Phy. aceris CGMCC 2.2662T; (C) Phy. jiayinensis CGMCC 2.5669T; (D) Me. layueensis CGMCC 2.5818T; (E) Sa. melibiophila CBS 5143T; (F) Mi. ellipsoideus CGMCC 2.5664T; (G, H) Mi. rubellus CGMCC 2.4444T; (I, J) Mi. pseudomagnisporus CGMCC 2.4538T; (K, L) Sy. rhododendri CGMCC 2.2613T; (M) Cy. raffinophilum CGMCC 2.3822T; (N) Cy. terricola CGMCC 2.3823T, (O) Do. ningxiaensis CGMCC 2.4451T; (P) Beg. foliicola CGMCC 2.3164T. Bars = 10 μm.