Macrobiotus hannae, Nowak & Stec & Online & Version, 2018

Nowak, Bernadeta, Stec, Daniel, Online, Published & Version, Final, 2018, An integrative description of Macrobiotus hannae sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae: hufelandi group) from Poland, Turkish Journal of Zoology 42 (3), pp. 269-286 : 272

Macrobiotus hannae sp. nov. ( Tables 3 and 4; Figures 1A–1E , 2A–2F , 3A–3E , 4A–4B , 5A–5D , 6A–6D , 7A–7F , 8A–8B)

3.2. Material examined

A total of 139 animals (including 4 in simplex stage) and 146 eggs. Specimens mounted on microscope slides in Hoyer’s medium (95 animals + 126 eggs), fixed on SEM stubs (20 + 20), and processed for DNA sequencing (4 + 0) and aceto-orcein staining (20 + 0).

Figures

Figure 1. Macrobiotus hannae sp. nov. – habitus and cuticle morphology: A – dorsoventral projection (holotype, Hoyer’s medium, PCM); B – pores in the cuticle on the dorsal side of the body seen in PCM (paratype); C–E – cuticular pores and fine granulation seen in SEM on the dorsal side of the body (paratype). Scale bars in µm.

Figure 2. Macrobiotus hannae sp. nov. – cuticular structures on legs: A – granulation on leg III (paratype, PCM); B – granulation on leg IV (holotype, PCM); C – granulation on leg I (paratype, SEM); D – granulation on leg IV (paratype, SEM); E – cuticular bulge resembling pulvinus-like structure on the internal surface of leg III (paratype, PCM); F – cuticular bulge resembling pulvinus-like structure on the internal surface of leg I (paratype, SEM); filled indented arrowheads indicate the granulation on the external surface of leg III and I, respectively; filled arrowheads indicate the cuticular bulge whereas empty arrowhead indicates faint cuticular bar under the claws. Scale bars in µm.

Figure 3. Macrobiotus hannae sp. nov. – buccal apparatus and the oral cavity armature seen in PCM (all paratypes): A – dorsoventral projection of the entire buccal apparatus; B–C – oral cavity armature of the hufelandi type (all three bands of teeth visible), dorsal and ventral view, respectively; D–E – placoid morphology, ventral and dorsal view, respectively. Filled indented arrowheads indicate teeth of the first band. Scale bars in µm.

Figure 4. Macrobiotus hannae sp. nov. – the oral cavity armature of a single paratype seen in SEM from different angles, A – dorsal side; B – ventral side. Filled indented arrowheads indicate teeth of the first band, empty indented arrowheads indicate teeth of the second band, the ridges of the third band are marked with “M” (median tooth) and “L” (lateral teeth). Scale bars in µm.

Figure 5. Macrobiotus hannae sp. nov. – claws: A–B – claws II (holotype) and IV (paratype) seen in PCM, with smooth and slightly dentate lunules respectively; C–D – claws I and IV seen in SEM, with smooth and slightly dentate lunules respectively. Filled flat arrowhead indicates a cuticular bar, filled indented arrowhead indicates indentation in lunules IV, empty arrowhead indicates the horseshoe structure connecting the anterior and the posterior claw. Scale bars in µm.

Figure 6. Macrobiotus hannae sp. nov. – egg seen in PCM:A – midsection under 400× magnification;B – surface under 400× magnification; C – midsection, to show processes, under 1000× magnification; D – surfaces under 1000× magnification. Scale bars in µm.

Figure 7. Macrobiotus hannae sp. nov. – egg chorion morphology seen in SEM: A – entire egg with faintly visible reticulation on the surface between processes; B–D – details of reticulation and processes arrangement on the egg surface; E–F – zoom on a single egg process and terminal disc respectively. Scale bars in µm.