Leptohyphes quimbaya Molineri, Zúñiga & Ramos, 2016

Molineri, Carlos, Zúñiga, María del Carmen, Ramos, Blanca C., Giraldo, Lina P. & Cardona, William, 2016, Three new species of Leptohyphes Eaton (Ephemeroptera: Leptohyphidae) from Colombia, Iheringia, Série Zoologia (e 2016024) 106, pp. 1-12 : 7-8

Leptohyphes quimbaya Molineri, Zúñiga & Ramos sp. nov.

( Figs 3 , 8-9 , 21-27 )

LSIDurn:lsid:zoobank.org:act: 7A14AC75-BF35-4979- 9AF9-533481E4109F

Type material. Holotype ♀ mature nymph (parts on slide), from COLOMBIA, Risaralda, La Celia, La Esmeralda farm, San Camilo creek , Monos river basin, 05°02’8.5”N, 75°59’46.7”W, 1648 m, microhábitat: cobble and gravel mixture, 11.II.2011, W. Cardona col. (MUSENUV). Paratypes: 1♂ mature nymph, same data as holotype (IBN); 1 nearly mature ♀ nymph, the same information except Monos river-La Linda creek , 05°00’40.5”N, 76°00’53.7”W, 1551 m, microhabitat: cobble and gravel, 8.II.2011 (MUSENUV); 1♂ immature nymph, the same information except La Pradera farm, La Reina creek , 04°57’52.7”N, 76°00’7”W, 1480 m, microhabitat: cobble and gravel, 15.II.2011 (MUSENUV) .

Additional material. Two ♂ and 2♀ immature nymphs from Risaralda, Santuario, Finca La Gaviota farm, La Gaviota creek , 05°02’39.8”N, 75°59’40.4”W, 1638 m, microhabitat: cobble and gravel, 12.II.2011, W. Cardona col. (2 in MUSENUV, 2 in IBN) ; 1♀ immature nymph from Risaralda, La Celia, San Camilo farm, San Camilo creek , 05°02’12”N, 75°59’34.9”W, 1779 m, microhabitat: stones, 10.II.2011, W. Cardona col. (MUSENUV) ; 4♀ immature nymphs, the same information except Verdum Regional Natural Park, Monos river , 05°00’56.2”N, 76°01’49.6”W, 1846 m, microhabitat: cobble and gravel, 5.II.2011 (MUSENUV) .

Diagnosis. 1) large, body covered by hair-like setae ( Figs 3 , 8, 9 ), with paired short and blunt tubercles on abdominal terga V-IX ( Figs 26, 27 ); 2) femora wide, fore femur ( Fig. 24 ) length/maximum width, 1.6 (mature nymphs) and 1.2 (immature nymphs), with mediolongitudinal row of spine-like setae distally to transverse row (“d.r.” in Fig. 24 ); 3) fore and hind margin of femora with tiny spine-like setae ( Figs 23, 24 ); 4) tarsal claws denticulation 2-3+0 ( Fig. 25 ); 5) pronotum with triangular (but apically blunt) lateral projection ( Fig. 8 , 22 ); 6) hind wing pads present in females; 7) gill formula 3/7/7/5/2, gill V without ventral extension on dorsal lamella.

Description. Mature nymph. Length of female (mm): body, 7.1-9.2; hind femur, 1.9-2.0; caudal filaments, 7.5. General coloration light brown, body and legs dorsally covered with whitish hair-like setae, venter glabrous. Head with black transverse band from eye to eye as in Fig. 21 ; occiput with finely marked gray net-pattern ( Fig. 21 ). Thorax: nota brownish with gray markings. Pronotum subrectangular with small lateral subtriangular projection, and an oblique indentation near posterior margin (sp and oi in Fig. 22 ). Mesonotum with anterolateral corners not projected ( Fig. 22 ). Hind wing pads present in female. Legs yellowish shaded dorsally with gray except mediolongitudinal narrow band on all femora ( Fig. 9 ). Foreleg ( Fig. 24 ): femur wide, ratio length/maximum width 1.6; transversal row of setae at 1/2 from base; fore margin with setae, hind margin with setae (on elevated sockets); apex of femur projected on inner and outer margins ( Fig. 24 ). Tibia subequal in length to femur with row of setae on inner margin; tarsus 0.4 the length of tibia with row of setae on inner margin; tarsal claw with two to three marginal denticles and none subapical submarginal denticle ( Fig. 25 ). Middle and hind legs ( Fig. 23 ) similar, except hind leg larger; hind femur ratio length / maximum width = 2; fore margin with 3-4 rows of short blunt setae, hind margin with 70 stout spine-like setae on elevated sockets (the sockets and the spines are very small), dorsal surface with many small scattered spine-like setae; distal projections on apex of femur more pronounced than foreleg. Tibia 1.1 the length of femur, with dorsal ridge, inner and outer margins with short weak spine-like setae. Tarsus 0.3 the length of tibia, inner margin with setae, tarsal claw as in foreleg. Abdomen ( Figs 26, 27 ) brownish shaded widely with gray, ventrally paler. Terga ( Figs 9 , 26 ) covered by whitish hair-like setae, mainly along hind margin; short blunt spine-like setae scattered on terga, mainly on submedian tubercles; segments III-VI with lateral flanges, small and poorly developed posterolateral projections on VII-IX; sternum IX with distomedial notch. Gills: operculate gill completely shaded with gray, except margins; other gills whitish shaded slightly with gray. Gill formula 3/7/7/5/2; gill V without ventral extension. Caudal filaments with scattered setae, and whorl of spines at joinings.

Variation: early instars of L. quimbaya only show groups of small setae on the area where abdominal tubercles will develop, and their almost circular forefemur does not present the mediolongitudinal row of spine-like setae distally.

Adults. Unknown.

Etymology. Quimbaya refers to an ethnic group that inhabited the valley of the Cauca river, between the Central and Western Cordilleras in Colombia, now the departments of Caldas, Quindío and Risaralda.

Distribution ( Fig. 30 ). Colombian Western Cordillera, Risaralda department, middle basin of Cauca River.

Discussion. The uncommon large body size together with the many tiny femoral spine-like setae, the setose body, very wide femora and paired abdominal tubercles readily distinguish this species from any other in the genus. Leptohyphes quimbaya is similar to L. pilosus Allen & Brusca, 1973 , mainly in the body pilosity, but the last species presents fewer and larger spine-like setae on slender femora. Abdominal tubercles are present in few other species of the genus, but only in L. nebulosus Nascimento et al., 2014 they are paired, nevertheless the later presents spine-like setae on very elevated sockets on femora, and is restricted to Brazilian coastal Atlantic forest ( NASCIMENTO et al., 2014).

Ecological notes. The species was collected in several creeks on the eastern slopes of the Western Cordillera in Colombia (Risaralda department). The landscape spans elevations from 1300 to 2000 m, where sun–grown coffee is dominant ( WCS, 2013) and corresponds to the transition zone between the Lower Montane Wet Forest and Premontane Moist Forest ( Espinal 1977). Mean annual rainfall is 2500 mm and mean annual temperature is 18°C. The landscape is a mosaic of vegetation types dominated by patches of native vegetation that differ in size (located at the top of the mountains and in glens), sun–grown coffee plantations, annual crops, stover and pasture ( WCS, 2013). The Verdum Municipal Natural Park in this study area is located in the buffer zone of the TatamÁ National Natural Park and it is important in environmental terms as a reservoir of Andean forest.

Figures

Figs 1-3. General habitus of nymph, dorsal: 1, Leptohyphes andina sp. nov.; 2, L. calarca sp. nov.; 3, L. quimbaya sp. nov. Scale bars = 1 mm.

Figs 4-9. Head/pronotum, and abdomen, dorsal: 4-5, Leptohyphes andina sp. nov.; 6-7, L. calarca sp. nov.; 8-9, L. quimbaya sp. nov.

Figs 21-27. Leptohyphes quimbaya sp. nov.: 21, dorsal color pattern of head; 22, outline (right side only) of pro- and mesonotum; 23, hind leg; 24, foreleg; 25, fore tarsal claw; 26, abdomen, dorsal (long setae omitted on right side); 27, abdomen, lateral (outline, setation omitted). Scale bars = 0.1 mm.

Fig. 30. Distribution map of Leptohyphes quimbaya sp. nov.