Jaagichlorella roystonensis var. handai Darienko & Pröschold, 2019

Darienko, Tatyana & Pröschold, Thomas, 2019, The genus Jaagichlorella Reisigl (Trebouxiophyceae, Chlorophyta) and its close relatives: an evolutionary puzzle, Phytotaxa 388 (1), pp. 47-68 : 64-65

Jaagichlorella roystonensis var. handai Darienko & Pröschold var. nov. ( Fig. 5I –G‘)

Description: Differs from the variety epilithica by slightly irregular cell shape and one-sided thickening of the cell wall. SSU and ITS rDNA sequences (GenBank: MH780942) and ITS-2 Barcode BC-3a in Fig. 3 .

Type locality: Bark of tree, Japan.

Holotype (designated here): The authentic strain SAG 2198 is cryopreserved in a metabolic inactive state at the Culture Collection of Algae ( SAG), University of Göttingen, Germany.

Iconotype (designated here to support the holotype): Fig. 5Q in this study.

Etymology: The name of this variety was given in honor of the isolator, Dr. S. Handa.

Comment: Differs from the type variety by ecology and differences in the variable regions of SSU and ITS rDNA sequences.

Jaagichlorella hainangensis (J. Zhang, V.A.R. Huss, X. Sun, K. Chang, & D. Pang) Darienko & Pröschold comb. nov. ( Fig. 6A–S )

Basionym: Heveochlorella hainangensis J. Zhang, V.A.R. Huss, X. Sun, K. Chang, & D. Pang, 2008 , Eur. J. Phycol. 43: 187.

Emended description: SSU and ITS rDNA sequences (GenBank: MH780943) and ITS-2 Barcode BC- 4 in Fig. 3 .

Comment: The morphological features of this species are identical to Jaagichlorella geometrica (see emended diagnosis below), but differs in ecology, origin and CBCs/HCBCs in the conserved region of the ITS-2 sequences.

Figures

FIGURE 3. Comparison of the conserved region of ITS-2 among the species of Jaagichlorella and Kalinella. Extraction of this region and translation into a number code for its usage as barcode. The ‘/’ indicated the position of the site loop in Helix III, which we excluded from the barcode. Number code for each base pair: 1 = A-U; 2 = U-A; 3 = G-C; 4 = C-G; 5 = G•U; 6 = U•G; 7 = pyrimidine-pyrimidine mismatch; 8 = deletion, single or unpaired bases. The phylogenetic tree shown was calculated using the neighbor-joining method based on the data set (69 number-coded positions of 11 haplotypes) using PAUP 4.0b10.

FIGURE 5. Morphology and phenotypic plasticity of Jaagichlorella roystonensis var. epilithica (SAG 2133; A.–H.) and J. roystonensis var. handai (SAG 2198; I.–G‘.); scale bar = 10 μm.

FIGURE 6. Morphology and phenotypic plasticity of Jaagichlorella hainangensis (SAG 2360; A.–S.) and J. geometrica (SAG 2549; T.–J’.); scale bar = 10 μm.