Hymenobacter ginkgonis, Cha & Kang & Kim & Bae & Joh, 2020

Cha, Inseong, Kang, Heeyoung, Kim, Haneul, Bae, Seokhyeon & Joh, Kiseong, 2020, Hymenobacter ginkgonis sp. nov., isolated from bark of Ginkgo biloba, International Journal of Systematic and Evolutionary Microbiology 70 (8), pp. 4760-4766 : 4763-4765

DESCRIPTION OF HYMENOBACTER GINKGONIS SP. NOV.

Hymenobacter ginkgonis (gink.go'nis. N.L. gen. n. ginkonis of Ginkgo biloba).

Cells are strictly aerobic, Gram-stain-negative, nonmotile, rod-shaped, approximately 2.3–3.9 µm long and 1.0–1.2 µm in diameter. Colonies on R2A agar are circular, smooth, pale-pink coloured and approximately 1.0 mm in diameter after 3 days at 30 °C. Growth occurs at 10–30 °C (optimum, 30 °C), at pH 6–8 (optimum, pH 7) and in the presence of 0–0.5% NaCl (optimum, 0%). Catalase and oxidase activities are positive. Flexirubin-type pigments are not produced. Growth does not occur on nutrient, marine, tryptic soy and blood agars. Dextrin is hydrolysed, but CM-cellulose, DNA, starch, casein (skimmed milk) or cellulose (filter paper) are not. In the API 20NE strip, positive for aesculin hydrolysis and β -galactosidase (PNPG test) activities, and the assimilation of D-glucose, D-mannitol, maltose, potassium gluconate, adipic acid and malic acid; negative for arginine dihydrolase, urease, nitrate reduction, indole production, glucose fermentation and gelatinase activities, and the assimilation of L-arabinose, D-mannose, N -acetylglucosamine, capric acid, trisodium citrate and phenylacetic acid. In the API ZYM strip, alkaline phosphatase, esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, β -galactosidase, α -glucosidase, β -glucosidase and N - acetylβ -glucosaminidase are present; esterase (C4), lipase (C14), α -chymotrypsin, α -galactosidase, β -glucuronidase, α -mannosidase and α -fucosidase activities are absent. In the API 50 CH strip, positive for acid production from aesculin ferric citrate; but negative for acid production from glycerol, erythritol, D-arabinose, L-arabinose, D-ribose, D-xylose, L-xylose, D-adonitol, methyl β -Dxylopyranoside, D-galactose, D-glucose, D-fructose, D-mannose, L-sorbose, L-rhamnose, dulcitol, inositol, D-mannitol, D-sorbitol, methyl α -D-mannopyranoside, methyl α -D-glucopyranoside, N -acetyl-glucosamine, amygdalin, arbutin, salicin, cellobiose, maltose, lactose, melibiose, sucrose, trehalose, inulin, melezitose, raffinose, starch, glycogen, xylitol, gentibiose, turanose, D-lyxose, D-tagatose,D-fucose,L-fucose,D-arabitol,L-arabitol, potassium gluconate, potassium 2-keto-gluconate and potassium 2-keto-gluconate. The major cellular fatty acids (>10%) are summed feature 3 (C ω7 c and/or C 16:1 ω6 c), iso-C and C 16:1 ω5 c. The only respiratory quinone is MK-7. The polar lipids contain phosphatidylethanolamine, one unidentified aminoglycolipid, three unidentified aminophospholipids, one unidentified phospholipid, three unidentified aminolipids, two unidentified glycolipids and three unidentified polar lipids. The genomic DNA G+C content of strain HMF4947 T is 59.3 mol%.

*Data from: a, Chung et al. [5]; b, Ten et al. [6]; c, Kang et al. [15]; d, Hoang et al. [33].

16:1

15:0

The type strain is HMF4947 T (=KCTC 72780 T =NBRC 114271 T), isolated from a ginkgo tree of the Yongin , Republic of Korea. The GenBank accession number for the 16S rRNA gene sequence of strain HMF4947 T is MN595016 . The whole genome accession number of strain HMF4947 T is WQKZ00000000.