Heteropriapulus anchoradiatus, Acosta, Aline Angelina, Franceschini, Lidiane, Zago, Aline Cristina, Scholz, Tomáš & Silva, Reinaldo José Da, 2017

Acosta, Aline Angelina, Franceschini, Lidiane, Zago, Aline Cristina, Scholz, Tomáš & Silva, Reinaldo José Da, 2017, Six new species of Heteropriapulus (Monogenea: Dactylogyridae) from South American fishes with an amended diagnosis to the genus, Zootaxa 4290 (3), pp. 459-482 : 468-469

Heteropriapulus anchoradiatus n. sp.

urn:lSid:zOObank.Org:act:4A6AFAA6-F135-44BF-A592-D04A452D4630 ( Fig. 4 )

Type and only known host. Pterygoplichthys ambrosettii (HOlmberg) ( SilurifOrmeS : LOricariidae ).

Site of infection. GillS.

Type locality. Aguapeí RiVer , municipality Of CaStilhO (Paraná RiVer baSin) , SãO PaulO State , Brazil (21°3'36.20"S, 51°45'38.58"W) .

Representative DNA sequence. 1,575 bp lOng Sequence Of the D1–D3 regiOn Of the lsr DNA Of One Specimen (AcceSS. NO. MF 116371 ) [paragenOphOre NO. IPCAS M-656].

Type specimens. HOlOtype (CHIOC 38697a); 9 paratypeS in CHIOC (38697b; 38698–38700; 38801–38805); 9 paratypeS in CHIBB (267-275L); 3 paratypeS in IPCAS (M- 656).

Etymology. The name iS frOm Latin and referS tO the preSence Of rOOtS in the baSe Of the Ventral anchOrS (anchor = anchOr + radiatus = rOOtS).

Description. BaSed On 22 SpecimenS: 19 mOunted in HOyer’S medium and 3 Stained With GOmOri’S trichrOme. BOdy 376–657 (504; n=19) lOng, greateSt Width 59–174 (109; n=19), uSually near mid-length. AcceSSOry granuleS eXtending frOm leVel Of gOnadS tO cephalic lObeS. Male cOpulatOry cOmpleX cOmpriSing nOn-articulated cirruS and acceSSOry piece. CirruS delicate, tOtal curVed length 86–141 (116; n=21), Straight in anteriOr pOrtiOn, ShOWing 1½ cOunterclOckWiSe Spiral ringS neXt tO the rOunded baSe. AcceSSOry piece 50–71 (60; n=21) lOng, cOmpOSed Of 2 StrOngly SclerOtized SubunitS nOt OVerlapping: One relatiVely Smaller Shaped aS a triangle With rOunded eXtremitieS and fOlding itSelf, and anOther lOnger irregularly Shaped With the preSence Of 2 lateral rami ( Fig. 4 D). Vaginal canal aS a lOng tube, SclerOtized and SigmOid, 35–60 (48; n=19) lOng, 61–95 (79; n=19) curVed length. Seminal receptacle pOuch-like and Weakly SclerOtized. EggS 66–103 (91; n=6) lOng, 41–73 (54; n=6) Wide, Operculate, ShOrt pOlar filament OppOSed tO Opercular end. HaptOr 51–108 (72; n=19) lOng, 78–147 (103; n=19) Wide, glObOSe tO Slightly SubtrapezOidal. Ventral anchOrS 28–33 (30; n=22) lOng, length tO nOtch 22–27 (24; n=22), Well-deVelOped Superficial rOOt 12–16 (14; n=22) lOng, ShOrt deep rOOt 6–8 (6; n=22) lOng, eVenly curVed ShOrt Shaft, pOint 14–17 (16; n=22) lOng With recurVed tip; anchOr filament dOuble and mOderately deVelOped; SclerOtized patch aSSOciated With the baSe Of Ventral anchOr abSent. DOrSal anchOrS 28–34 (31; n=22) lOng, length tO nOtch 22–27 (24; n=22), Well-deVelOped Superficial rOOt 13–17 (16; n=22) lOng, ShOrt deep rOOt 3–6 (4; n=22) lOng, eVenly curVed ShOrt Shaft, Straight pOint 14–16 (15; n=22) lOng, anchOr filament dOuble and mOderately deVelOped. Ventral bar 60–77 (67; n=22) lOng, 6–11 (8; n=22) Wide, 11–22 (18; n=22) high, bent, rOunded endS. DOrSal bar 28–37 (33; n=22) lOng, 3–5 (4; n=22) Wide, 20–27 (23; n=22) high, With rObuSt pOSterO-medial prOceSS 12–16 (13; n=22) Wide. SeVen pairS Of Similar hOOkS 15–17 (16; n=22) lOng, With rOund, Weakly SclerOtized Subunit at baSe, FH lOOp abOut ½ Of Shank length.

Remarks. COnSidering the Original generic diagnOSiS prOVided by JOgunOOri et al. (2004), the haptOral elementS Of thiS neW SpecieS differ frOm thOSe Of Heteropriapulus Spp., but the elementS Of the male cOpulatOry cOmpleX fall intO thOSe Of the Original generic diagnOSiS. LikeWiSe, H. simplex dOeS nOt preSent elementS Of the male cOpulatOry cOmpleX cOmplying With the Original generic diagnOSiS, but preSentS haptOral elementS that fall intO the Original generic diagnOSiS. NOnetheleSS, the mOlecular data preSented in thiS Study StrOngly SuppOrt generic placement Of bOth Heteropriapulus anchoradiatus n. sp. and H. simplex ( Fig. 10 ).

Heteropriapulus anchoradiatus n. sp. differS frOm H. heterotylus and H. simplex (and alSO all Other SpecieS Of the genuS deScribed aS neW in the preSent paper—See belOW) in the pOSSeSSiOn Of a lOng, SigmOid SclerOtized Vagina. BeSideS, H. anchoradiatus n. sp. pOSSeSSeS Ventral anchOrS With ShOrt Shaft and preSence Of Superficial and deep rOOtS, WhereaS all Other SpecieS Of Heteropriapulus knOWn SO far haVe Ventral anchOrS With an elOngate Shaft and a flattened baSe. ThiS neW SpecieS iS alSO characterized by haVing a cOnSpicuOuS pOSterO-medial prOceSS On the dOrSal bar, Which iS incOnSpicuOuS, ShOrt Or eVen abSent in cOngenerS. All hOOk pairS preSent a rOund, Weakly SclerOtized Subunit at the baSe Of the hOOkS, Which iS abSent in the cOngenerS. Heteropriapulus anchoradiatus n. sp., Similarly aS H. simplex , dOeS nOt preSent SclerOtized patch aSSOciated With the Ventral bar.

Figures

FIGURE 4. (A – H) Sclerotized structures of Heteropriapulus anchoradiatus n. sp. from Pterygoplichthys ambrosettii (Holmberg) from the Upper Paraná River, Brazil. A, ventral anchor. B, dorsal anchor. C, vagina. D, male copulatory complex. E, egg. F, hook. G, dorsal bar. H, ventral bar.

FIGURE 10. Maximum likelihood topology based on partial 28 S rDNA sequences of selected monogenean species. Newly sequenced species from this study are in dark blue. Posterior probabilities followed by bootstrap support values are given above the branches. Pseudorhabdosynochus epinepheli, P. lantauensis and Pseudomurraytrema sp. were used as outgroups. Branch length scale bar indicates number of substitution per site.