Haematopinus apri

Nie, Yu, Fu, Yi-Tian, Wang, Wei, Li, Rong, Tang, Wan-Qing & Liu, Guo-Hua, 2022, Comparative analyses of the fragmented mitochondrial genomes of wild pig louse Haematopinus apri from China and Japan, International Journal for Parasitology: Parasites and Wildlife 18, pp. 25-29 : 27-28

3.4. Comparative mt genomic analyses of H. apri from China and Japan

A comparison of the nucleotide and the amino acid sequences of mt genes for H. apri from China and Japan are given in Table 2. The nucleotide sequence variation across the entire mt genome (except for non-coding regions) between the wild pig louse H. apri from China and

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mitochondrial genes between the wild pig louse H. apri from China (HaC) and from Japan (HaJ) upon pairwise comparison.

Japan was 1.7%. Difference across both nucleotide and amino acid sequences of the 13 protein-coding genes between the wild pig louse H. apri from China and Japan was 1.7% and 1.4%, respectively. The magnitude of nucleotide sequence difference in each gene between the wild pig louse H. apri from China and Japan ranged from 0.8% to 2.4%. The greatest difference between the wild pig louse H. apri from China and Japan was nad 2 gene, whereas the least difference was detected in the rrn S gene ( Table 2). Amino acid sequences inferred from individual mt protein-coding genes of wild pig louse H. apri from China and Japan were also compared. The amino acid sequence differences ranged from 0.4% to 3.5%, with cox 3 gene being the most conserved protein-coding gene and nad 3 gene the least conserved. Previous studies of other lice have also detected low intra-specific sequence variation in mt sequences. For example, the intra-specific sequence variation in P. pubis was 0–1.5% for p cox 1 (775 bp) ( Amanzougaghene et al., 2020) and 1.0% for p cyt b (696 bp) ( Light and Reed, 2009; Shao et al., 2012). The intra-specific sequence variation in P. badii was less than 0.3% for p cox 1 (346 bp) ( Light et al., 2010). Taken together, the molecular evidence presented here indicated the wild pig louse H. apri from China and Japan represent the same species.