Girdia agilis subsp. samples

Lyu, Zhangxia, Cheng, Jiaoni, Shao, Jingru, Ye, Qingqing, Bai, Huixian & Wen, Jianfan, 2020, An investigation of the prevalence of Giardia agilis in anuran amphibians from fourteen areas in China, International Journal for Parasitology: Parasites and Wildlife 12, pp. 46-52 : 49

3.4. Phylogenetic analysis of collected G. agilis samples

We sequenced a fragment of the SSU rRNA and beta-giardin genes of the collected G. agilis samples , respectively. Genetic distances between G. agilis and other Giardia species at the two loci are equal or greater than the differences between currently accepted Giardia species ( Table 6). And G. agilis is most genetically close to G. intestinalis and G. microti . Phylogenetic analysis based on these fragments showed that G. agilis we collected is genetically distinct from all other Giardia species ( Fig. 3A and Fig. 4A ), and that all G. agilis we tested showed a small genetic distance, indicating they should be from same species despite the samples being collected from different hosts and areas ( Figs. 3B and 4B ).

Figures

Fig. 3. Phylogenetic tree of SSU rRNA Phylogenetic tree of SSU rRNA, A. G. agilis is distinct from all Giardia species; B. all G. agilis we tested were from the same species.

Fig. 4. Phylogenetic tree of beta-giardin Phylogenetic tree of beta-giardin, A. G. agilis is distinct from all Giardia species; B. all G. agilis we tested were from the same species.