Filobasidium mali Q.M. Wang, F.Y. Bai & A.H. Li, 2020

Li, A. - H., Yuan, F. - X., Groenewald, M., Bensch, K., Yurkov, A. M., Li, K., Han, P. - J., Guo, L. - D., Aime, M. C., Sampaio, J. P., Jindamorakot, S., Turchetti, B., Inacio, J., Fungsin, B., Wang, Q. - M. & Bai, F. - Y., 2020, Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species, Studies In Mycology 96, pp. 17-140 : 100-103

Filobasidium mali Q.M. Wang, F.Y. Bai & A.H. Li sp. nov. MycoBank MB828789. Figs 10P and 11A .

Etymology: the specific epithet mali refers to the substrate origin of the type strain, Malus .

Culture characteristics: In YM broth, after 7 d at 17 °C, cells are subglobosal and ellipsoidal, 3.0– 4.6 × 3.0– 7.7 μm and single, budding is polar ( Fig. 10P ), a sediment is present. After 1 mo at 17 °C, a ring and a sediment are present. On YM agar, after 1 mo at 17 °C, the streak culture is gray-cream, mucoid, smooth and shiny. The margin is entire. In Dalmau plate culture on corn meal agar, pseudohyphae are not formed. Sexual structures are not observed on YM, PDA, V8 and CM agar. Ballistoconidia are not produced.

Physiological and biochemical characteristics: Glucose fermentation is absent. Glucose, Galactose, L-sorbose, sucrose, maltose, cellobiose (or weak), trehalose, melibiose (or weak), raffinose (or weak), melezitose (or weak), D-xylose (or delayed and weak), L-arabinose (or weak), L-rhamnose (or delayed and weak), ethanol (or weak), D-mannitol, ribitol, galactitol, Methylα- D-glucoside (or weak), salicin (or weak), D-Gluconate (weak), succinate (delayed and weak) and myo-inositol (delayed and weak) are assimilated as sole carbon sources. Lactose (variable), inulin, soluble starch, D-arabinose (variable), D-ribose, Dglucosamine, N-Acetyl-D-glucosamine, methanol, glycerol, Dglucitol (variable), erythritol, DL-lactate, citrate and hexadecane are not assimilated. Ammonium sulfate, potassium nitrate (weak), L-lysine, and cadaverine dihydrochloride are assimilated as sole nitrogen sources. Sodium nitrite and ethylamine hydrochloride (variable) are not assimilated. Maximum growth temperature is 32 °C. Growth in vitamin-free medium is negative. Starch-like substances are produced or not. Growth on 50 % (w/ w) glucose-yeast extract agar is negative. Urease activity is positive. Diazonium Blue B reaction is positive.

Physiologically, Fi. mali differs from its closely related species Fi. globosum in its inability to grow in vitamin-free medium and its ability to assimilate ribitol, galactitol and salicin ( Table S1.15 ).

Typus: China, Tai’ an county, Shandong province, obtained from isolated from apple, Aug. 2008, Q.-M. Wang (holotype CGMCC 2.4012 T preserved in a metabolically inactive state, ex-type CBS 15651 = KTAPG4-11.64).

Figures

Fig. 10. Vegetative cells grown in YM broth for 5 d at 17 °C and ballistoconidia produced on corn meal agar after 7 d at 17 °C. (A, B) De. melastomatis CGMCC 2.3459T; (C, D) De. taiwanicus CGMCC 2.4429T; (E, F) De. ovatus CGMCC 2.3572T; (G, H) De. longicylindricus CGMCC 2.5660T;; (I) Pha. lactea CGMCC 2.5810T; (J) Pha. ovata CGMCC 2.5614T; (K, L) Ho. saccardoi CGMCC 2.3445T; (M) So. gelidoterrea CGMCC 2.5814T; (N) Fi. dingjieense CGMCC 2.5649T, (O) Fi. globosum CGMCC 2.5680T; (P) Fi. mali CGMCC 2.4012T. Bars = 10 μm.

Fig. 11. SEM image of vegetative cells grown in YM broth for 5 d at 17 °C. (A) Fi. mali CGMCC 2.4012T, Bars = 4 μm; (B) Boe. sterigmata CGMCC 2.4539T, Bars = 5 μm; (C) St. layueensis CGMCC 2.5817T, Bars = 5 μm; (D) Pse. motuoensis CGMCC 2.5816T, Bars = 2 μm; (E) Me. layueensis CGMCC 2.5818T, Bars = 5 μm; (F) Beg. foliicola CGMCC 2.3164T, Bars = 1 μm; (G, H) Ros. petaloides CGMCC 2.3446T, G Bars = 10 μm, H Bars = 3 μm.