Eisenia muuido Blakemore, 2015

Blakemore, Robert J., Lee, Seunghan & Seo, Hong-Yul, 2015, Account of montane and insular speciation in some Korean megadriles (Annelida: Oligochaeta), Journal of Species Research 4 (1), pp. 1-22 : 14-15

17. Eisenia muuido Blakemore sp. nov. ( Fig. 10 ).

Material. IV0000261296 (DNA HY30) H, holotype, a mature specimen from Muuido (same details as IV 000 261298) collected 15 th Sept. 2013 by RJB; IV0000261 297 P, paratype, a subadult lacking tail; plus an immature specimen (S).

Etymology. After island locality (noun in apposition).

Description. Pale body, pinkish in life with distinct yellow anterior and posterior tips (coelomocyctes as in E. japonica ) found in all life stages (H, P, S). Length 100 mm (H) or 55+ mm (P) with 120 segments. Open epilobous. First dorsal pore 4/5. Clitellum weak saddle in ca. 27-32,33. TP elongate bands lateral of b setae on 28-32. Setae ab on 28-32 tumid. Spermathecal pores minute in 9/10/11 dorsally as rounded sacs (one heart-shaped). Female pores in 14 just lateal of b setae. Male pores on barely marked porophores also just lateral of b setae. Nephropores not found. Holandric: testis in seminal vesicles in 9-10 medium sized and larger in 11-12. Hearts in 7-11. Calciferous glands annular in 11 & 12. Ovaries in 12 as flattened tongues with many eggs. Intestine from 1 / 215. Crop in 16. Gizzard in 17-18. Typhlosole not noted. Nephridial bladders sausage-shaped. Much mucous produced when handled.

Remarks. From Michaelsen (1900), the closest agreement is with Eisenia tigrina (Rosa, 1896) from Europe, however it has first dorsal pore in 3/4 and much larger male pores (14/15-15/16). Of the twenty Korean lumbricids listed in Blakemore (2014), the geographically closest are Eisenia japonica (Michaelsen, 1892) and its sub-species (as described by Blakemore & Grygier, 2011; Blakemore, 2012c; 2013) that have clitella around 24-31 and TP restricted to 27 & 29 or E. koreana (Zicsi, 1972) , E. gaga Blakemore, 2012 , and E. sindo Blakemore, 2012 all three of which differ, however, by having clitella near 25-32 and TP around 27-29. Several of these taxa are genetically tested in Appendix and show matches no closer than 79-84% thus helping to define the uniqueness of the current worm.

Blakemore, R.J. 2000. Tasmanian Earthworms. CD-ROM Monograph with Review of World Families. VermEcology, PO BOX 414 Kippax 2615. Canberra, December, 2000. pp. 800 including 222 figures.

Blakemore, R.J. 2002. Cosmopolitan earthworms - an ecotaxonomic guide to the peregrine species of the World. VermEcology, Kippax, Australia. pp. 506 [CD publication].

Blakemore, R.J. 2003. Japanese earthworms (Annelida: Oligochaeta): a review and checklist of species. Organisms, Diversity and Evolution 3 (3): 241-244 [Available from: http://www.urbanfischer.de/journals/ode/; www.sencken berg.de/odes/03-11.htm].

Blakemore, R.J. 2006. A series of searchable texts on earthworm biodiversity, ecology and systematics from various regions of the world - Supplemental. Second CD-Rom publication under rules of ICZN (1999). Eds. N. Kaneko & M. T. Ito. COE Soil Ecology Research Group. Yokohama National University, Japan. - 3rd Edition (2008): http://www.annelida.net/earthworm/ (accessed 13/03/ 2013).

DNA barcode Blast results show match no closer than 85-86% for Eisenia nordenskioldi aff. nordenskioldi (Eisen, 1879) (Genbank JX531498.1 from a Russian study) and its sub-species as redescribed by Blakemore (2013c: tab. 1) that have clitella in the region of 26,27- 32,33 and TP 29-31, thus closest to the current taxon. In Korean Allolobophora species reviewed by Blakemore (2013c: tab. 2), viz. Allolobophora hataii , A. harbinensis and A. dairenensis all by Kobayashi (1940), their clitella are in region of 23,24,25-32,33 and TP in 29-31 only, thus they too differ morphologically.

Figures

Fig. 10. Eisenia muuido sp. nov. Holotype showing spermathecae, nephridium 18rhs and calciferous glands in 11 & 12.