Dyella dinghuensis, Ou & Gao & Chen & Bi & Qiu, 2019

Ou, Fang-hong, Gao, Zeng-hong, Chen, Mei-hong, Bi, Jie-yi & Qiu, Li-hong, 2019, Dyella dinghuensis sp. nov. and Dyella choica sp. nov., isolated from forest soil, International Journal of Systematic and Evolutionary Microbiology 69 (5), pp. 1496-1503 : 1501-1502

DESCRIPTION OF DYELLA DINGHUENSIS SP. NOV.

Dyella dinghuensis (ding.hu.en′ sis. N.L. fem. adj. dinghuensis referring to Dinghu Mountain, where the organism was isolated).

Cells are Gram-stain-negative, aerobic, non-motile, and rod-shaped (0.3–0.6×1.0–2.3 µm). Colonies are circular, convex with clear margin and yellow-coloured after 4 days on R2A agar. Growth was observed on trypticase soy agar, R2A agar and Luria–Bertani agar, but not MacConkey agar. Growth on R2A agar occurs at 12–37 Ǫ C, pH 4.5–7.0 and in the presence of 0–1.0 % (w/v) NaCl. Optimum growth occurs at 28 Ǫ C, pH 5.0–6.0 and without NaCl supplement. Catalase-positive and oxidase-positive. Nitrate is not reduced to nitrite. Casein is hydrolysed, but gelatin, starch and Tween 80 are not. H 2 S and indole are not produced. The following substrates can be used as sole carbon sources for growth: adipic acid, arbutin, amygdalin, D- xylose, D- galactose, D- glucose, D- fructose, D- mannose, D- mannitol, cellobiose, maltose, inulin, malic acid, methyl Oi- D- glucopyranoside, N -acetyl-D- glucosamine and phenylacetic acid. The following substrates are not utilized for growth: aesculin ferric citrate, capric acid, D- adonitol, D- arabinose, D- arabitol, D- fucose, D- gentiobiose, lactose, D- lyxose, melezitose, melibiose, raffinose, D- ribose, D- sorbitol, sucrose, D- tagatose, trehalose, turanose, dulcitol, erythritol, glycerol, glycogen, inositol, L- arabinose, L- arabitol, L- fucose, L- rhamnose, L- sorbose, L- xylose, methyl Oi - D- mannopyranoside, methyl β - D- xylopyranoside, potassium 2-ketogluconate, potassium 5- ketogluconate, potassium gluconate, salicin, starch and xylitol. The following enzyme activities are positive: alkaline phosphatase, acidphosphatase, arginine dihydrolase, cystine arylamidase, Oi -galactosidase, Oi -glucosidase, Oi -fucosidase, Oi -mannosidase, β -galactosidase, β -glucosidase, esterase (C4), esterase (C8), leucine arylamidase, naphthol-AS-BIphosphohydrolase, N -acetyl- β -glucosaminidase, urease and valine arylamidase. Trypsin is weakly positive. The following enzyme activities are negative: Oi -chymotrypsin, β -glucuronidase and lipase (C14). Ubiquinone-8 is the only respiratory quinone, and iso-C 15: 0, iso-C 16: 0, iso-C 17: 0, iso- C 17: 1 Ɯ 9 c are the major cellular fatty acids (>10 %). The G+C content of the genomic DNA is 59.1 mol%. The major polar lipids consist of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine, one unidentified aminophospholipid, two unidentified aminolipids, one unidentified phospholipid and lipid.

The type strain, DHOA06 T (=KCTC 52129 T =NBRC 111978 T), was isolated from forest soil sampled at Dinghushan Biosphere Reserve , Guangdong Province, PR China. The 16S rRNA gene sequence and whole genome sequence of strain DHOA06 T have been deposited in DDBJ/EMBL/ GenBank under accession numbers KY194795 and RYZR00000000, respectively.