Distichlicoccus takumasae, Caballero, 2021

Caballero, Alejandro, 2021, Four new scale insect species (Hemiptera: Coccomorpha) associated with coffee roots in Colombia, South America, with identification keys for genera Newsteadia Green, 1902, Distichlicoccus Ferris, 1950, and Paraputo Laing, 1929, Zoosystema 43 (18), pp. 341-363 : 348-352

Distichlicoccus takumasae n. sp.

( Figs 3 ; 4 )

urn:lsid:zoobank.org:act:174B1078-53DE-4AEE-9323-84D8DA99806D

TYPE MATERIAL. — Holotype. Colombia • adult ♀ (2 specimens on the same slide, holotype situated on the left side and indicated as “ Holo ” on an additional label); Cauca, El Tambo, Vereda Villanueva, Farm Patio Bonito ; 1824 m a.s.l.; 5.IX.2015; J. Muñoz leg.; ex roots of Coffea arabica (Rubiaceae) – coffee, manual;: UNAB 4621 .

Paratypes. Colombia • 1 adult ♀; same data as holotype; marked as “Para” sharing a slide with the holotype; UNAB 4621 • 1 adult ♀; same data as holotype; paratype mounted singly on a slide; MNHN .

ETYMOLOGY. — The specific epithet “ takumasae ” is assigned in honor of Dr Takumasa Kondo, mentor of the author.

DIAGNOSIS. — Circulus absent; cerarii numbering 15-17 pairs; oral rim tubular ducts few, numbering 4-9 on dorsum and 3 or 4 on venter; and oral collar tubular ducts restricted to venter of SabdIII-VII.

DESCRIPTION

Pre-mounting specimens

Appearance in situ: not observed.

Post-mounting specimens

Slide-mounted adult female (n = 3): body oval to round ( Fig. 3 ; 4A ), length 1.5 ± 0.04 [1.5] (1.4-1.5) mm; wide 1.2 ± 0.05 [1.1] (1.1-1.2) mm.

Dorsum

Ostioles. Conspicuous, with membranous lips.

Anal ring. 66.2 ± 2.4 [68.8] (64-68.8) µm in transverse diameter, with 6 flagellate setae, each seta 104.8 ± 10.7 (83.6- 122) µm long; ring containing two cells rows, external row with cells smaller than those in internal row, subquadrate and subrectangular, each cell with a spicule 2 or 3 µm long; internal row with irregular cells tending to be elongate and without spicules.

Cerarii. Numbering 15-17 pairs ( Fig. 3A ), C 10 and C 14 absent or vestigial [16 pairs in holotype, with left side C 10 and C 14 vestigial]; cerarian pattern C 1 [2;3-4] (2;3-4), C 2 [2;2] (2;1-3), C 3 [2;3] (2;1-3), C 4 [2;1] (2;0-2), C 5 [2;2-3] (2;0- 3), C 6 [2;1-3] (2;0-3), C 7 [2-3;2-3] (2-3;1-3), C 8 [1-2;0-1] (1-2;0-1), C 9 [2;1] (2;0-1), C 10 [abortive;0-1] (0-1;0-2), C 11 [1;1-2] (1-2;0-2), C 12 [2-3;0-1] (1-2;0-2), C 13 [2;1-3] (1-2;0-3), C 14 [0-3;0-3] (1-3;0-3), C 15 [2-3;0-1] (2-3;0-1), C 16 [2;1-3] (2-3;1-3), C 17 [2;0] (0-2;0-1).

Body setae. Flagellate. Each 13.5 ± 2.5 (7.7-24.2) µm long, shorter than ventral setae, evenly distributed.

Trilocular pores. Each 3-4 µm in diameter ( Fig. 3B ), evenly distributed.

Multilocular disc pores. Absent.

Oral rim tubular ducts. Of one size ( Fig. 3C , 4B ), each 12.1 ± 1.6 (10-15) µm long and associated with 1-2 discoidal pores, some associated with setae; distributed as follows: mesothorax [1] (0-1) in lateral region, metathorax [1] (1) in submesial region, SabdI [3] (1-3) in marginal and submarginal regions, SabdII [2] (1-2) in submesial region, SabdIII [2] (0-2) in marginal region, SabdIV [0] (0-1) in submarginal region, absent from remaining segments.

Oral collar tubular ducts. Absent.

Discoidal pores. Of two types: I) with a single locule, similar size or slightly smaller than a trilocular pore, 2.9 ± 0.4 (2-5) µm in diameter ( Fig. 3D ), scarce, evenly scattered; II) with two loculi, 3.5 ± 0.6 (3-5) µm in diameter ( Fig. 3E ), scarce, absent from SabdI, SabdIV and SabdVIII + IX. Body spicules present on head, prothorax and S abdIII and posterior segments.

Venter

Antennae. Each 341 ± 13 (327-360) µm long, 7 or 8 segmented ( Fig. 3F ; 4C ); holotype with 7 antennal segments, one antenna with SantIV and SantV partially divided and other one with SantIII and SantIV partially divided, segment length and chaetotaxy as follows: SantI 55.3 ± 4.0 (49-61) µm long, with four flagellate setae; SantII 48.4 ± 6.6 (35-53) µm long, with six flagellate setae and one placoid sensilla; SantIII 36.9 ± 10 (21-51) µm long with six to eight flagellate setae; SantIV 29.6 ± 13.5 (22-50) µm long with four or five flagellate setae; SantV 33.0 ± 9.6 (25-50) µm long with six to eight flagellate setae; SantVI 28.1 ± 2.3 (25-32) µm long with five or six flagellate setae; SantVII 38.2 ± 1.5 (35-40) µm long with five to seven flagellate setae and one fleshy seta; and SantVIII 86.7 ± 2.8 (83-92) µm long with 18-19 flagellate setae and four fleshy setae [holotype with 11 and 15 flagellate setae].

Eyespot. 33.7 ± 2.9 (29-37) µm in diameter, not associated with discoidal pores ( Fig. 4D ).

Labium. 126.3 ± 8.7 [not measured] (120-132) µm long.

Fore leg. 499 ± 5.5 (489-504) µm long, trochanter + femur 233.5 ± 3.2 (230-238) µm long, femur 74.4 ± 10.4 (56-80) wide; tibia + tarsus 235.2 ± 3.3 (230-238) µm long, tibia 35.5 ± 2.3 (32-38) µm wide; claw 30.3 ± 1.2 (29-32) µm long; ratio of lengths of trochanter + femur length/tibia + tarsus 1.0; ratio of trochanter + femur length/femur width 3.2 ± 0.6 (2.9-4.2), ratio of tibia + tarsus length/tibia width 6.7 ± 0.5(6.2-7.3); chaetotaxy: coxa with nine flagellate setae, trochanter with seven, femur with 25-29, tibia with 23-29, tarsus with 11 setae and two slender and capitate tarsal digitules, each 36.4 ± 4.7 (28-39) µm long; claw without a denticle but with two ungual digitules each 25.7 ± 1.5 (24-28) µm long, capitate, slightly thicker than a tarsal digitule. Mid leg. 504.7 ± 5.7 (497-512) µm long; trochanter + femur 232 ± 2.3 (229-234) µm long, femur 72.1 ± 11 (58-80) µm wide; tibia + tarsus 241.3 ± 3.6 (236-245) µm long, tibia 38.6 ± 5.9 (31-45) µm wide; claw 31.3 ± 1.9 (29-33) µm long; ratio of lengths of trochanter + femur: tibia + tarsus 1.0; ratio of trochanter + femur length: femur width 3.3 ± 0.5 (2.9-4.0), ratio of tibia + tarsus length: tibia width 6.4 ± 1.0 (5.4-7.7); chaetotaxy: coxa with 11 flagellate setae, trochanter with seven, femur with 23-26, tibia with 29-31, tarsus with 11 setae and one sensilla; tarsal digitules similar those on fore tarsus, 36.2 ± 2.1 (33-39) µm long; claw without a denticle, ungual digitules each 25.2 ± 0.8 (24.1-26) µm long, similar those on fore claw.

Hind leg. 551.7 ± 30.3 (501-574) µm long; trochanter + femur 255.2 ± 10.7 (234-263) µm long; femur 73 ± 8.7 (61- 80) µm wide; tibia + tarsus 266 ± 20.6 (237-281) µm long; tibia 41.1 ± 4.4 (37-49) µm wide; claw 30.5 ± 2.7 (27-33) µm long; ratio of lengths of trochanter + femur/tibia + tarsus 1.0 ± 0.1 (0.9-1.1); ratio of trochanter + femur length/femur width 3.5 ± 0.5 (3.0-4.3), ratio of tibia + tarsus length/tibia width 6.5 ± 0.8 (5.6-7.5); translucent pores distributed as follows: coxa with 8 ± 8 [absent] (0-18), femur with 59 ± 10 (45-72), tibia with 46 ± 10 (29-59) ( Figs 3G ; 4E ); chaetotaxy: coxa with 11 flagellate setae, trochanter with seven, femur with 20-23, tibia with 29-35, tarsus with 10 setae and one sensilla; tarsal digitules each 33.7 ± 2.2 (31.0-37.0) µm long, similar those on fore tarsus; claw without a denticle, ungual digitules each 24.0 ± 1.9 (22-26) µm long, similar to those on other legs.

Circulus. Absent.

Anal lobes ( Fig. 4F ). Prominent, slightly sclerotized, each with apical flagellate seta 114 ± 8 (109-120) µm long and with 2-4 auxiliary flagellate setae.

Body setae. Flagellate, lengths variable: on head, 46 ± 8 (34- 58) µm long, prothorax 22 ± 8 (11-40) µm, mesothorax 34 ± 6 (28-44) µm, metathorax 23 ± 7 (15-39) µm; on abdominal segments there are two groups setae: I) shorter setae, each 21 ± 5 (11-29) µm long, evenly distributed, II) longer setae, each 37 ± 5 (30-48) µm long, present in mesial region. Intersegmental areas lacking setae.

Obanal setae. Each 34 ± 5 (28-40) µm long.

Cisanal setae. Each 36 ± 4 (28-40) µm long.

Trilocular pores. Each 3-4 µm in diameter, evenly distributed ( Fig. 3H ).

Multilocular disc pores. Absent, except for one paratype, which has three pores on SabdVII, each pore about 9 µm in diameter ( Figs 3I ; 4G ).

Discoidal pores. Of two types: I) with a single loculus 3.6 ± 0.5 (2-5) µm in diameter, few, evenly distributed ( Fig. 3J ); II) with two loculi, pore 4.3 ± 0.4 (3-5) µm in diameter, few ( Fig. 3K ).

Oral rim tubular ducts. Slightly smaller than dorsal ducts, each 11 ± 1.8 (8-14) µm long, with one on anterior interantennal margin and 1 or 2 in lateral or submarginal regions of mesothorax and SabdI ( Fig. 3L ).

Oral collar tubular ducts. Each 8 ± 3.6 (5-11) µm long ( Figs 3M ; 4H ), distributed as follows: SabdIII [3] (2-3), Sab- dIV [18] (12-18), SabdV [20] (16-20), SabdVI [17] (17), and SabdVII [13] (9-13).

Spicules. Present on mesothorax and posterior body segments.

REMARK

Distichlicoccus takumasae n. sp. is morphologically close to D. salazari Williams & Granara de Willink, 1992 in lacking a circulus and in having translucent pores on both the hind femur and tibia. However, D. takumasae n. sp. differs from D. salazari (characteristics in parenthesis) by having oral rim tubular ducts few, numbering 4-9 on dorsum and 3-4 on venter (has 2-7 oral rim tubular ducts in rows across each segment on both surfaces); oral collar tubular ducts present on venter but absent from dorsum (oral collar ducts on both venter and dorsum); and hind legs with translucent pores on coxa, femur and tibia (pores only on femur and tibia) [character data of D. salazari taken from Williams & Granara de Willink (1992)].

Figures

FIG. 3. — Taxonomic illustration of Distichlicoccus takumasae n. sp. adult ♀: enlarged details with measurements correspond to: A, cerarius; B, dorsal trilocular pore; C, dorsal oral rim tubular duct; D, dorsal simple discoidal pore; E, dorsal bilocular discoidal pore; F, antenna; G, hind femur and tibia; H, ventral trilocular pore; I*, multilocular disc pore, present in only one paratype; J, ventral simple discoidal pore; K, ventral bilocular pore; L, ventral oral rim tubular duct; M, ventral oral collar tubular duct. Scale bars: B, D, H, J, K, 3 µm; A, C, E, I, L, M, 5 µm; F, G, 50 µm.

FIG. 4. — Microphotographs of female specimens of Distichlicoccus takumasae n. sp.: A, body; B, oral rim tubular duct in lateral and surface views; C, antenna; D, eyespot; E, hind leg with translucent pores (arrows); F, anal lobe; G, multilocular disc pore; H, oral collar tubular duct. Scale bars: A, 300 µm; B, G, H, 5 µm; C, 30 µm; D, 10; E, 40 µm; F 20 µm.