Cytospora pruinopsis C.M. Tian & X.L. Fan, Mycological Progress 14(9): 74 (2015)

Zhu, Haiyan, Pan, Meng, Bezerra, Jadson D. P., Tian, Chengming & Fan, Xinlei, 2020, Discovery of Cytospora species associated with canker disease of tree hosts from Mount Dongling of China, MycoKeys 62, pp. 97-121 : 97

Cytospora pruinopsis C.M. Tian & X.L. Fan, Mycological Progress 14(9): 74 (2015) Fig. 6

Description.

See Yang et al. (2015).

Material examined.

China, Beijing City, Mentougou District, Mount Dongling, Xiaolongmen Forestry Centre (115°27'29.37"E, 39°56'47.49"N), from branches of Ulmus pumila , 22 Aug 2017, H.Y. Zhu & X.L. Fan, CF 2019806, living culture CFCC 53153.

Habitat and distribution.

Known from Ulmus pumila in Northern China.

Notes.

Yang et al. (2015) described Cytospora pruinopsis from cankers of Ulmus pumila in Shannxi Province of China. The strain CFCC 53153 clusters in a well-supported clade with high support value (MP/ML/BI = 100/100/1), based on combined multi-locus gene phylogenetic analyses (Fig. 2 ). Morphologically, it confirms Cytospora pruinosa in having a single locule and small conidia (2-4 × 1 μm) as per the descriptions of Yang et al. (2015). Phylogenetically, our isolates represent 6/771 nucleotide differences of tef1-α comparing with ex-type strains CFCC 50034 of C. pruinosa . Morphology and sequence data confirmed that our isolates represent this species.

Figures

Figure 2. Phylogram of Cytospora, based on combined ITS, LSU, act, rpb 2, tef 1 - α and tub 2 genes. The MP and ML bootstrap support values above 50 % are shown at the first and second positions, respectively. Thickened branches represent posterior probabilities above 0.95 from the BI. Ex-type strains are in bold. Strains from the current study are in blue.

Figure 6. Cytospora pruinopsis from Ulmus pumila (CF 2019806). A, B habit of conidiomata on twig C transverse section of conidiomata D longitudinal section through conidioma E conidiophores and conidiogenous cells F conidia G colonies on PDA at 3 days (left) and 30 days (right). Scale bars: 1 mm (A); 250 μm (B); 500 μm (C, D); 10 μm (E, F).