Cyphellophora guizhouensis W. Sun & M.C. Xiang, 2019

Sun, Wei, Liu, Bingjie, Fu, Rong, Liu, Xingzhong & Xiang, Meichun, 2019, Two new rock-inhabiting species of Cyphellophora from karst habitats in China, Phytotaxa 397 (1), pp. 23-33 : 29

Cyphellophora guizhouensis W. Sun & M.C. Xiang , sp. nov. ( FIGURE. 3 .)

MycoBank no.: MB829501

Etymology:— Guizhouensis (Lat.) referring to Guizhou province, China, the location of the fungus isolated.

Type:— China, Guizhou Province, Anshun city, Tianlong Town, 26°22.285′N, 106°10.048′E, alt. 1372m a.s.l., isolated from rock, 15, Nov., 2014, Meichun Xiang, HMAS 247967 (holotype), CGMCC 3.19234 = LB1.16010182 (ex-type cultures). GenBank accession number: MK116364 (ITS), MK116375 (nucLSU).

Description:—Colonies reaching 28 mm diam after 4 week at 25 °C, consisting of dense cottony texture, pale grey in the centre progressively to medium yellowish brown near the margin; reverse olivaceous dark brown. No diffusible pigment produced. Hyphae thin to slightly thick-walled, smooth-walled, loosely septate, hyaline to subhyaline, 1.0–2.0 μ m wide. Collarettes funnel-shaped, collarettes occasionally sessile on intercalary hyphal cells. Conidia, non-septate, hyaline to pale brown, ranging from obovoidal to ellipsoidal, clavate, cylindrical, often truncate at the basal end, 4.0– 8.0 ×.2.0–3.5 μm (⎯ x = 5.2×2.5 μ m, n=10). Budding cells present. Chlamydospore and sexual morph not observed.

Cardinal temperatures:—Optimum growth temperature 20–27 °C, minimum below 4 °C, maximum 29 °C.

Additional specimens examined:— China, Guizhou Province, Anshun city, Tianlong Town , 26°22′285′′N, 106°10′048′′E, 1372m a.s.l., isolated from rock, 15, Nov., 2014, Meichun Xiang, CGMCC 3.19235 = LB1.160101250 ; ibid. CGMCC 3.19236 = LB1.160101251 .

Note:— Cyphellophora guizhouensis showed a close phylogenetic relationship with C. pauciseptata (93% identity in ITS; 98% identity in nucLSU) ( Fig. 1 ). However, morphological differences of C. guizhouensis vs. C. pauciseptata in producing smooth (vs. pauciseptata) and shorter and wider conidia (4.0–8.0 × 2.0–3.5 μ m vs. 14–16 × 0.8–1.0 μ m) ( Feng et al. 2014). C. guizhouensis is also morphologically similar to C. sessilis , however its conidia is longer (4.0–8 μm vs. 2.3–4.1 μm) ( Caretta et al. 2006, Réblová et al. 2013).

Figures

FIGURE 1. Phylogenetic tree of Cyphellophora based on the combined data set of ITS and nucLSU with Phaeomoniella prunicola as outgroup. Clades with Bayesian probabilities>0.95 and bootstrap support values>60% are indicated at the nodes (ML/BI). For the Bayesian inference, the best-fit model (GTR+I+G with ingamma-distributed rate) for ITS and nucLSU were selected in MrModeltest 2.3. The phylogenetic tree obtained from Bayesian analyses was using MrBayes 3.0b4. Thickened branches represent posterior probabilities (>0.95) from BI. The scale bar indicates 0.1 substitutions per site. The two new species from this study are marked in bold. The type specimens are indicated with an T.

FIGURE 3. Cyphellophora guizhouensis (CGMCC 3.19234). a,b. Colony on MEA after 4 weeks at 25 °C; c. Spirally twisted hypha; d–h. Conidiogenous cells and conidia (arrows point to conidiogenous cells); i. Conidia and budding cell. Scale bars = 10 μm.