Clistobothrium

Klotz, Daniela, Hirzmann, Jörg, Bauer, Christian, Schöne, Joachim, Iseringhausen, Maximilian, Wohlsein, Peter, Baumgärtner, Wolfgang & Herder, Vanessa, 2018, Subcutaneous merocercoids of Clistobothrium sp. in two Cape fur seals (Arctocephalus pusillus pusillus), International Journal for Parasitology: Parasites and Wildlife 7 (1), pp. 99-105 : 102

3.3. Phylogenetic analyses confirm assignment to Clistobothrium

The new 18S rDNA sequence and the D2 region of the 28S rDNA — identical to the grimaldii-type merocercoid sequence — were used for phylogenetic analyses using a dataset of 17 and 25 sequences covering 15 phyllobothriidean genera ( Fig. 4 , Supplementary Table 1). In the 28S D2 rDNA analysis, the merocercoid from the fur seal, the delphini- and grimaldii-type merocercoids and the LP plerocercoids group in one clade with adults of the two Clistobothrium species C . montaukensis and C. carcharodoni as shown previously by several authors ( Agusti et al., 2005a; Aznar et al., 2007; Jensen and Bullard, 2010; Randhawa, 2011; Randhawa and Brickle, 2011). This assignment to Clistobothrium was also verified using the near complete 18S rRNA gene for analysis, although with a smaller number of available taxa.

Figures

Fig. 4. Phylogenetic trees of Clistobothrium sp. merocercoids from the Cape fur seal and related phyllobothriid species based on the 18S and 28S D2 rDNA regions using maximumlikelihood method. Nodal support is indicated by bootstrap values in percent; scale: number of substitutions per site; country and accession no. after the species name.