Botryllus flavus, Sanamyan, Karen & Sanamyan, Nadya, 2017

Sanamyan, Karen & Sanamyan, Nadya, 2017, Shallow-water Ascidians from Matua Island (central Kuril Islands, NW Pacific), Zootaxa 4232 (3), pp. 301-321 : 307-310

Botryllus flavus n. sp.

( Figures 6 , 7 )

Botryllus sp. Sanamyan, 2000: 76.

Botryllus magnus: Sanamyan & Sanamyan, 2010: 245 (Kamchatka) . Not B. magnus Ritter, 1901: 255 (Alaska) . Sanamyan, 2000: 76 (Commander Islands).

Material examined. Holotype: KBPGI 1449 /1, Kuril Islands , Matua Island, Point Kluv, 17 m, 25.08.2016 . Paratypes: KBPGI 1450 /2, Matua Island, Point Kluv , 15 m, 26.08.2016, one colony.

Additional material examined. Pacific coast of Kamchatka, Starichkov Island, 28.07.2004, 19 m, one colony ( Figures 6 C, D) ; 21.09.2004, 20 m, one colony; Kuril Islands , Atlasova Island, 17 m, 26.07.1989, one colony.

Description. The species forms extensive sheets encrusting stones. The colony of the holotype is 8 x 5 cm in extent and from 5 to 10 mm thick (preserved). The surface is clear, sometimes with some sediment attached on the periphery of the colony or between the systems, but usually free from foreign matter ( Figure 6 ). The common tissue of the colony may be depressed between some systems, or even completely reduced between some groups of systems, so that the colony usually is not represented by one large continuous sheet but looks rather like a group of several large and small sheets joined together ( Figures 6 C, D). Numerous elongated crowded ampullae are present on the periphery and, in a lesser quantity, between the systems in colonies from Kamchatka ( Figure 6 C, D); in the colonies from Matua Island (the type material) they are sparse. The colouration of live specimens is very constant. All recorded colonies are yellow with a reddish tint underwater. The colour is monotonous, there are no any colour markings over zooids, etc., as often seen on some other Botryllus species. Upon collecting, on the air, live colonies quickly become deep-orange or bright red probably because of oxidation of the pigment they contain. In formaline they also become red, then the red pigment is washed out, and during several weeks the fluid become deep dirtyreddish and the colony loses deep colour.

Perfectly preserved, not contracted zooids are somewhat larger than 3 mm in height (in holotype). They are upright, standing perpendicular to the surface of the colony. The systems are circular or oval, of " schlosseri " type (see Brunetti, 2009), clearly visible on the surface of colony, each may contain as many as 13 or 14 zooids, but often the number of zooids in a system is less than ten. Branchial openings are on short circular (not lobed) siphons which in live colonies arise slightly over the surface of the colony ( Figure 6 A). Atrial orifices are small, on the end of sometimes rather long atrial siphon. Atrial siphons of all zooids belonging to one system open independently into a small central cloacal cavity.

The oral tentacles are eight in number, four of which are longer (two lateral, dorsal and ventral) and four shorter ( Figure 7 B).The branchial sac has 15 or 16 rows of stigmata, often 15 on the right and 16 on the left. The second row of stigmata, on both sides of the branchial sac, is not complete. An example of distribution of stigmata between three internal longitudinal vessels, counted in the middle of the branchial sac is follow: 12-9-6-6 -DL- 7-6- 7–10.

The stomach is short, cylindrical, both its ends (pyloric and cardiac) are of about the same diameter. Longitudinal stomach folds are prominent, not swollen on the cardiac end, run diagonally, ten in number (the typhlosolis not counted), of which fold #9 (see Figure 7 C) is always short and does not reach cardiac end of the stomach, and the fold #10 is rudimentary and not always present. The typhlosolis extends over the pyloric end of the stomach. The caecum, arising from the typhlosolis on the level of pyloric end of the stomach is rather long, about half length of the stomach, bent, and swollen into a spherical ampulla at the end. The secondary loop is widely open, the short straight rectum makes an obtuse angle with the intestine. The anus is bilobed, with the smooth margin.

In all colonies examined the zooids of the first (filtering) generation contain no gonads. In the holotype the colony contains also small zooids of the second, and attached to them minute zooids of the third generation. The latter contain up to five ova on each side of the body. Male gonads were not detected. Colonies contain no larvae.

Remarks. This is the only known Botryllid species occurring in the region from east coats of Kamchatka to north and central Kuril islands . It was reported previously from Pacific coast of Kamchatka by Sanamyan & Sanamyan (2010) as Botryllus magnus and from Paramushir Island ( north Kuril Islands ), as Botryllus sp. by Sanamyan (2000).

Botryllus magnus was originally described by Ritter (1901) from Kodiak and Popof Islands, Alaska. Van Name (1945) inclined to believe that B. magnus (reported by him as Botrylloides magnum), is identical with northern and Arctic species Botrylloides aureum Sars, 1851 , but we do not support this view: the shape of the systems is an obvious and valid character separating Botrylloides aureum from Botryllus magnus . A colony reported as Botryllus magnus by Sanamyan (2000) from Commander Islands is probably identified correctly: the colony, the shape of system and the zooids are similar with those described by Ritter (1901).

Zooids of Botryllus flavus n. sp. differ from B. magnus in the following points: 1) The secondary gut loop in B. flavus n. sp. is widely open, while in B. magnus it is more narrow (the intestine makes a sharp angle with the rectum, as figured by Ritter, 1901, Figure 36 or described as "narrow and nearly closed" by Sanamyan, 2000: 76). 2) The branchial sac of B. magnus from Commander Islands and from Alaska has 11–13 rows of stigmata, while 15 or 16 rows are present in B. flavus n. sp. 3) The specimen of B. magnus from Commander Island has the second row of stigmata complete (a feature not described for the specimens from Alaska), while in B. flavus n. sp. the second row is not complete on both sides of the branchial sac. 4) Both Ritter (1901) and Sanamyan (2000) reported 16 tentacles for B. magnus . The presence of 16 tentacles in the material from Commander Islands was confirmed by reexamination, the first two cycles of tentacles (4+4) are distributed in the same way as described above for B. flavus n. sp., but B. magnus has also a third ring of eight small tentacles. We cannot decide at this point if this is a valid species specific feature. Finally, the two species appear to have different colour. Ritter (1901) specially pointed that his colonies showed practically no variation in colour from colony to colony. All his colonies were "dusky purple", probably of the same colour as Sanamyan's (2000) material from Commander Islands (described as dark blue in live) although it is not known if Ritter's description applied to live or to preserved specimens. Botryllus flavus n. sp., on the other hand, is always uniformly bright yellow-reddish, the species is not abundant but not rare in Kamchatka waters and we had many chances to observe colonies underwater during many years of diving activity, no color variations were encountered.

Several botryllids were described or are know from more southern and warmer waters along Pacific coasts of Russia and Japan, but none of them is similar with B. flavus n. sp., and none of them occurs in central Kuril Islands or in Kamchatka waters.

Figures

FIGURE 6. Botryllus flavus n. sp., colonies. A, paratype KBPGI 1450 / 2; B, holotype KBPGI 1449 / 1; C and D, specimens from Kamchatka.

FIGURE 7. Botryllus flavus n. sp. A, zooid; B, tentacles; C, gut loop.