Belnapia soli

Maeng, Soohyun, Kim, Ju-Young, Jang, Jun Hwee & Kim, Myung-Suk Kang and Myung Kyum, 2018, A report of 11 unrecorded bacterial species in Korea isolated in 2017, Journal of Species Research 7 (2), pp. 135-150 : 137-139

Description of Belnapia soli 17SD2_15

Cells are Gram-stain-negative, non-flagellated, and coccus-shaped. Colonies are pale pinkish-colored after 3 days of incubation on R2A at 25°C. In the BIOLOG GEN III, D-glucuronic acid was utilized as a sole carbon source. But acetoacetic acid, N -acetyl-D-mannosamine, L-alanine, L-arginine, L-aspartic acid, D-cellobiose, dextrin, D-fructose, D-fructose 6-PO 4, L-galactonic acid lactone, D-galactose, D-galacturonic acid, gelatin, gentiobiose, D-gluconic acid, α -D-glucose, glucuronamide, L-glutamic acid, inosine, α -keto-glutaric acid, L-malic acid, D-maltose, D-mannitol, D-mannose, D-melibiose, β -methyl-D-glucoside, 3-methyl glucose, myo-inositol, pectin, propionic acid, glycyl-L-proline, L-pyroglutamic acid, quinic acid, D-raffinose, L-rhamnose, L-serine, stachyose, sucrose, D-trehalose, D-turanose, acetic acid, N -acetyl-D-galactosamine, N -acetyl-neuraminic acid, N -acetyl-D-glucosamine, γ -amino-butryric acid, D-arabitol, D-aspartic acid, bromo-succinic acid, citric acid, formic acid, D-fucose, L-fucose, D-glucose-6- PO 4, glycerol, L-histidine, α -hydroxybutyric acid, β -hydroxy-D, L-butyric acid, p -hydroxy-phenylacetic acid, α -keto-butyric acid, L-lactic acid, D-lactic acid methyl ester, α -D-lactose, D-malic acid, methyl pyruvate, mucic acid, D-saccharic acid, D-salicin, D-serine, D-sorbitol, and tween 40 were not utilized. In sensitivity tests, the tetrazolium redox dye was reduced at pH 6 but not at 1% NaCl, 1% sodium lactate, 4% NaCl, 8% NaCl, guanidine HCl, lithium chloride, pH 6, potassium tellurite, D-serine, sodium butyrate, aztreonam, fusidic acid,

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lincomycin, minocycline, nalidixic acid, niaproof 4, pH 5, rifamycin SV, sodium bromate, tetrazolium blue, troleandomycin, and vancomycin.

In API 20NE system, positive for arginine dihydrolase and urease. Negative for nitrate reduction, indole production, glucose fermentation, esculin hydrolysis, gelatin hydrolysis, and β -galactosidase. Uses D-glucose, potassium gluconate, adipic acid, malic acid, and trisodium citrate but not L-arabinose, D-mannose, D-mannitol, N -acetyl-glucosamine, capric acid, D-maltose, and phenyl acetic acid as carbon sources.

In API 32GN system, positive for itaconic acid, suberic acid, sodium malonate, sodium acetate, lactic acid, L-serine, propionic acid, capric acid, potassium 2-ketogluconate, 3-hydroxybutyric acid, 4-hydroxybenzoic acid, and L-proline. Negative for L-rhamnose, N -acetyl-glucosamine, D-ribose, inositol, D-saccharose (sucrose), D-maltose, L-alanine, potassium 5-ketogluconate, glycogen, 3-hydroxybenzoic acid, D-mannitol, D-glucose, salicin, D-melibiose, D-fucose, D-sorbitol, L-arabinose, valeric acid, trisodium citrate, and L-histidine. Strain 17SD2_15 (= NIBRBAC000500510) was isolated from a soil sample.