Balantioides coli

K, Pamela C., oster, Lapuente, Juan, Pizarro, Andrea, Prieto-P, Laura, erez, Ramon, erez-Tanoira, Dashti, Alejandro, Begona ˜ Bailo, Muadica, Aly S., Gonz, David, alez-Barrio, Calero-Bernal, Rafael, Ponce-Gordo, Francisco & Carmena, David, 2022, Presence and genetic diversity of enteric protists in captive and semi-captive non-human primates in coteˆd’Ivoire, Sierra Leone, and Peru, International Journal for Parasitology: Parasites and Wildlife 17, pp. 26-34 : 28

2.11. Molecular detection of Balantioides coli

We carried out B. coli detection by a direct PCR assay to amplify the complete ITS1–5.8s-rRNA–ITS2 region and the last 117 bp (3 ′ end) of the ssu -rRNA sequence of this ciliate using the primer set B5D/B5RC ( Ponce-Gordo et al., 2011). PCR reactions (25 μL) consisted of 2 μL of template DNA and 0.4 μM of each primer ( Table S1). PCR conditions were as follows: 94 ◦ C for 10 min; 30 cycles of 94 ◦ C for 1 min, 60 ◦ C for 1 min, 72 ◦ C for 1 min, and a final extension for 5 min at 72 ◦ C.