Ariadna reginae, Giroti & Brescovit, 2018

Giroti, André Marsola & Brescovit, Antonio Domingos, 2018, The taxonomy of the American Ariadna Audouin (Araneae: Synspermiata: Segestriidae), Zootaxa 4400 (1), pp. 1-114 : 87-90

Ariadna reginae new species

Figures 54‒55 , 68

Type material: ♂ holotype from 4km SE Puerto Viejo de Sarapiqui, Finca La Selva , Puerto Viejo de Sarapiqui [10°27'20"N; 84°00'20"W], Heredia Province, Costa Rica, IX.1981, C.E. Griswold leg. ( CAS 9046566 ) . Paratypes: 1♂ 2♀ from the same locality of the holotype, 5‒20.IX.1981, C.E. Griswold leg. ( CAS 9046549 ) ; 1♂ from 11km ESE La Virgen [10°23'52.5''N; 84°08'09.8''W], 250‒300m, Sarapiquí, Heredia Province, Costa Rica, 18.IV.2004, INBio-OTS leg. ( INBIO 4407641 ) ; 1♀ from 11km ESE La Virgen [10°23'52.5''N; 84°08'09.8''W], Sarapiquí, Heredia Province, Costa Rica, 22.II.2004, INBio-OTS leg. ( INBIO 4407639 ).

Additional material examined: MEXICO: Chiapas State: 8km SE Salto de Agua , 100m, secondary wet forest (17°30'57.31"N; 92°18'08.31"W), 1♀, 14.IV.2008 ( MCZ 80237) ; GUATEMALA: Petén Department: Tikal Natural Park , 270m, tropical moist forest (17°14'25.21"N; 89°37'15.38"W), 1♀ 3imm., 22.V.2009 ( MCZ 89477) ; BELIZE: Cayo District: Augustine, Rio Frio near Augustine (16°58'N; 88°58'W), 1♀, 20.VII.1972, C. & M. Goodnight leg. ( AMNH) ; COSTA RICA: Guanacaste Province: La Cruz, 9km S de Santa Cecilia [11°03'40.5''N; 85°24'43.0''W], Estación Pitilla , 700m, 1♂, 1‒31.X.1996, C. Moraga leg. ( INBIO 4407608 ) ; Estación Cacao, 2km SW de Cerro Cacao [10°56'02.8"N; 85°27'01.8"W], 1100‒1200m, 1♂, VI.1990, A. Solis leg. ( INBIO 4407605 ) ; Alajuela Province: San Ramón, Upala [10°43'53.8''N; 85°03'36.8''W], Bijagua, San Miguel, Finca Inti-Aura, 300‒400m, 1♀, 15.X‒8.XII.2012, I. Chacón leg. ( INBIO 4407626 ) ; Guatuso, Parque Nacional V. Tenorio [10°40'23''N; 85°00'54''W], Punto 1, Catarata Rio Buenavista , 800m, 1♀, 9‒11.VI.2010, J.A. Azofeifa & M. Moraga leg. ( INBIO 4407620 ) ; San Ramón, Monteverde, Sendero Brilliante, 1600‒1625m (10°18'N; 84°47'W), 2imm., 28‒31.I.1999, J. Zujko-Miller leg. (INBIO 4407888); Heredia Province: Sarapiquí, Estación Biológica La Selva [10°25'50.1''N; 84°00'24.0''W], 0‒100m, 1♀, 20.VI.2011, INBio-OTS leg. ( INBIO 4407886 ) ; 1imm., IX.1996 (INBIO 4407885); Sarapiquí, Estación Biológica La Tirimbina [10°25'02.2''N; 84°07'31.9''W], 2♂, 10.VII‒13.VIII.2013, I. Chacón leg. ( INBIO 4407622 ) ; Sarapiquí, 11km ESE La Virgen [10°23'52.5''N; 84°08'09.8''W], 300‒400m, 1♂, 9‒21.II.2001, INBio-OTS leg. ( INBIO 4407884 ) ; 1imm., 5‒21.XI.2001 (INBIO 4407883); 1♀, 21.III.2003 (INBIO 4407880); 1♂, 20.IV.2003 (INBIO 4407881); 1♂, 9.III.2011 (INBIO 4407882); Sarapiquí, 11km ESE La Virgen [10°23'52.5''N; 84°08'09.8''W], 450‒550m, 1♂, 11.III.2003, INBio-OTS-ALAS leg. ( INBIO 4407640 ) ; Puerto Viejo de Sarapiquí, La Selva Biological Station (10°25'19"N; 84°00'54"W), 1♀, 13.III.1987, D.M. Olson leg. ( MCZ 125 213) ; Catargo Province: Turrialba, Reserva Florestal Río Pacuare [9°58'11.4''N; 83°27'48.4''W], Parque Nacional Barbilla, Estación Barbilla, 600m, 1imm., 20‒24.X.2000, W. Arana leg. ( INBIO 4407625 ) ; Limón Province: Veragua Rainforest [09°55'25.8''N; 83°11'23.9''W], 1♂, 9.IV‒20.V.2010, R. Villalobos leg. ( INBIO 4407624 ) ; Hone Creek [9°39'14.6''N; 82°48'00.5''W], Finca de Alberto Moore, 3♀, 14‒17.V.2004, C. Víquez leg. ( INBIO CRI); 1imm., 21‒23.X.2005 ( INBIO CRI); Reserva Biológica Hitoy Cerere [9°38'53.0''N; 83°04'02.8''W], 1imm, 14‒25.III.2003, E. Rojas et al. leg. ( INBIO 4407611 ) ; San José Province: San Antonio de Escazú [09°53'25"N; 84°08'38"W], 1725m, 1♀, 3.IX.1984, W. Eberhard leg. ( MCZ 40632) ; Cerro de Escazu [9°52'06.7''N; 84°09'29.6''W], 1500m, 1imm., X.1 987, W. Eberhard leg. ( MCZ 70282) ; Puntarenas Province: Aguirre [9°28'58.2''N; 84°05'41.2''W], Finca de Lucía Lutz , 100‒150m, 1♀ 2imm., 25.V.2010, M. Solís leg. ( INBIO 4407623 , 4408771 , 4408772 ) ; Reserva Privada Karen Mogensen [09°52'11.1''N; 85°03'28.4''W], Sendero el Viejo Níspero , 300‒400m, 1♀, 23.III.2003, Y. Cardenas et al. leg. ( INBIO 4407619 ) ; Estación Pittier, 4.2km SO de Cerro Gemelo [9°03'00.0''N; 82°55'58.8''W], 1670m, 1♀, 8.VI.1995, IV C. Paratax leg. ( INBIO 4407606 ) ; Parque Nacional Corcovado [08°33'N; 83°35'W], Quebrada Hedionda , 100m, 1♂, 16.III‒6.IV.2003, K. Caballero et al. leg. ( INBIO 4407612 ) .

Diagnosis. Ariadna reginae n. sp. is similar to A. obscura , A. tarsalis , A. arthuri , A. multispinosa and A. aurea n. sp. due to the morphology of the male metatarsi I ( Figs 16C‒E , 32C‒E , 38C‒E , 54C‒E , 56C‒E ) and macrosetae pattern of the tibia I‒II of females, but males differ by the presence slightly sinuous metatarsi I, with a ventroprolateral apophysis in the distal region, bearing a thickened macrosetae ( Fig. 54C‒E ); females differ, except A. obscura , by the presence of a ventral lobe with expanded tip. It differs from A. obscura by presenting sinuous lobes and smaller glandular ducts plate ( Fig. 55C‒D ).

Etymology. The specific name is in honor of Regina Maria Marsola Giroti, mother of André Marsola Giroti, who, together with his father João Jair Giroti, has given him the gift of life, struggling to provide the means for a good life and education.

Description. Male (Holotype). Carapace and chelicerae orange ( Fig. 54A ). Endites light orange; labium orange. Sternum light orange, with darker margins ( Fig. 54B ). Palps yellow ( Fig. 55A‒B ). Legs brownish yellow, with pairs I‒II darker. Dark pigment filling the femur, patella and tibia of legs I‒IV. Metatarsus and tarsus lighter, compared to the other leg articles ( Fig. 54C‒E ). Abdomen dark grey, with ventral region lighter ( Fig. 54A‒B ). Total length 2.84; carapace 1.38 long, 1.02 wide. Anterior lateral eyes near the anterior margin of the carapace, projecting anteriorly. Chelicerae not projected ( Fig. 54A ). Palps: tibia slightly incrassated, with the dorso-ventral diameter shorter than the bulb; bulb rounded, mid-portion and embolus of equal length; embolus tubular, curved and tapered, with slightly twisted tip ( Fig. 55A‒B ). Legs: robust and coated with setae ( Fig. 54C‒E ); tarsal brush of putative chemosensory setae present in legs II‒IV. Leg formula: I-II-IV-III. Leg mearsurements: I, femur 1.44, patella 0.56, tibia 1.26, metatarsus 1.28, tarsus 0.4, total 4.94; II, 1.44, 0.52, 1.22, 1.24, 0.46, 4.88; III, 1.1, 0.44, 0.78, 0.84, 0.42, 3.58; IV, 1.2, 0.56, 1.0, 0.94, 0.42, 4.12. Macrosetae: I, femur d0-0/1-1-0-1-0-2, p0-0-0-0-0-2/1-1; tibia d0-0-0-0-1/0-0-0, p1/0-1-0-1-0-1-0, vp1-0-1-0-1/0-0-1, vr1-0/1-1-0-1-0-1, r1-0-1-0-1-0-1; metatarsus p0-0/1- 0-0-0-0-0, vp1-0-0-1(seed-shaped/apophysis)-0-0-1(cylindrical shaft with abrupt pointed tip), vr0-0-0-1-0-0-1; II, femur d0-0-1/0-1-1-0-2, p0-0-0-0-0-1/0-1; tibia p0-1-0-1-0-1-0, vp0-0-0-0-0-0-0/1, vr2-0-1-0-1-0-1, r1-1-0-1-0-1- 0; metatarsus vp1-0-0-1-0-0-1, vr1-0-0-1-0-0-1, r0-1-0-1-0-0-0; IV, femur d0-1/0-0-1-1-1-0, r0-0-0-0-0-1-0; tibia vp0-1-0-0-0-0-0; metatarsus vp0-0-0-0-0-0-1, vr metatarsal comb with 4 macrosetae.

Female (Paratype—CAS 9046549). Coloration of carapace, chelicerae, endites, labium, and abdomen as in male ( Fig. 54F‒G ). Palps brownish yellow, gradually darkening to distal direction. Legs orange, with pairs I‒II darker, and dark pigment filling the distal region of the femur and patella, and tibia in its entirety; metatarsus and tarsus without dark pigment ( Fig. 54H‒I ). Total length 4.6; carapace 1.7 long, 1.14 wide. Carapace sparsely coated with setae. Chelicerae not projected ( Fig. 54F ). Legs: robust and coated with setae; legs I‒II with ventro-prolateral and ventro-retrolateral tibial and metatarsal macrosetae of different sizes ( Fig. 54H‒I ). Leg formula: I-II-IV-III. Leg measurements: I, femur 1.26, patella 0.62, tibia 1.0, metatarsus 0.9, tarsus 0.36, total 4.14; II, 1.16, 0.52, 1.0, 0.88, 0.36, 3.92; III, 0.88, 0.5, 0.64, 0.62, 0.34, 2.98; IV, 1.2, 0.58, 0.86, 0.72, 0.34, 3.7. Macrosetae: I, femur D0-0- 0-0-0-2-0, P0-0-0-0-1-0-0; tibia P0-1-0-1-0-1-0, vp1-1-1-1-1-1-0, vr1-1-1-1-1-1-1, R0-1-0-1-0-1-0; metatarsus vp1-1-1-1-1-1-1-1, vr1-1-1-0-1-1-1-1 ( Fig. 54H‒I ); II, femur D0-0-0-0-0-2-0, P0-0-0-0-0-1-0; tibia P0-1-0-1-0-1- 0, vp1-0-1-0-1-0-1, vr1-1-1-1-1-1-1; metatarsus vp1-1-1-1-1-1-1, vr1-1-0-1-1-1-1; IV, metatarsus vr metatarsal comb with 3‒4 macrosetae. Internal genitalia: anteriror receptaculum with the dorsal lobe shorter than the ventral lobe, and glandular ducts plate located in the median region of the receptaculum, with the proximal region expanded ( Fig. 55C‒D ).

Variation. Male (n=10): Total length 2.8‒3.72; carapace 1.38‒1.92 long, 1.02‒1.42 wide; femur I 1.4‒1.76. Female (n=10): Total length 2.88‒6.56; carapace 1.6‒2.7 long, 1.04‒1.82 wide; femur I 1.06‒1.94.

Distribution. Mexico, Guatemala, Belize and Costa Rica ( Fig. 68 ).

Figures

FIGURE 16. Ariadna obscura (Blackwall, 1858). Males from Fortaleza (MPEG 11628) (A‒B) and Mata São João (IBSP 85421) (C‒E): habitus, dorsal (A) and ventral view (B); right leg I, prolateral (C) and retrolateral view (D), arrowhead = lanceolated macroseta; right metatarsus I, ventral view (E), arrowhead = group of short distinct setae. Female from Fortaleza (IBSP 165434) (F‒I): habitus, dorsal (F) and ventral (G) view; left leg I, prolateral (H) and retrolateral view (I). Scale bar: 1mm.

FIGURE 32. Ariadna tarsalis Banks, 1902. Male from Playa Ibbetson (RBINS) (A‒E): habitus, dorsal (A) and ventral view (B); right leg I, prolateral (C) and retrolateral view (D), arrowhead = lanceolated macroseta; right metatarsus I, ventral view (E). Female from N-flank, Isla Santiago (RBINS) (F‒I): habitus, dorsal (F) and ventral view (G); left leg I, prolateral (H) and retrolateral view (I). Scale bar:1mm.

FIGURE 56. Ariadna aurea n. sp.. Male (Holotype) from Gruta dos Brejões (IBSP 151451) (A‒E): habitus, dorsal (A) and ventral view (B); rightleg I, prolateral (C) and retrolateral view (D); rightmetatarsus I, ventral view (E), arrowhead= truncated macroseta.Female (Paratype) from Grutasdos Brejões (IBSP 151451) (F‒I):habitus, dorsal (F) and ventral view (G); rightleg I, prolateral (H) and retrolateral (I). Scale bar: 1mm.

FIGURE54. Ariadna reginae n. sp.. Male (Paratype) from 11km ESE La Virgen (INBIO 4407641) (A‒E): habitus, dorsal (A) and ventral view (B); rightleg I, prolateral(C) andretrolateral view (D); right metatarsus I,ventral view (E).Female(Paratype) from 4km SE Puerto Viejo de Sarapiqui (CAS 9046549) (F‒I): habitus, dorsal (F) and ventral view (G); right leg I, prolateral (H) and retrolateral view (I). Scale bar:1mm.

FIGURE55. Ariadna reginae n. sp.. Male (Paratype) from 11km ESE LaVirgen (INBIO4407641) (A‒B):left palp, prolateral (A) and retrolateral view (B). Female (Paratype) from 4km SE Puerto Viejo de Sarapiqui (CAS 9046549) (C‒D): internal genitalia, lateral view (E); anterior receptaculum, ventral view (F). Scale bar: 1mm.

FIGURE68. Locality of the specimens of A.caerulea, A.tovarensis, A. arthuri, A.multispinosa, A.isthmica and A. reginae n. sp. examined in the present study.

FIGURE38. Ariadna arthuri Petrunkevitch, 1926.Male from Sierra de Cubitas (IBSP 166771) (A‒E):habitus, dorsal (A) and ventral view (B); left leg I, prolateral (C) and retrolateral view (D); left metatarsus I, ventral view (E). Female from Sierra de Cubitas (IBSP 166771) (F‒I): habitus, dorsal (F) andventral view (G); rightleg I, prolateral (H) and retrolateral view (I). Scale bar:1mm.