Ancylus sp.

Falniowski, Andrzej, Lewarne, Brian, Rysiewska, Aleksandra, Osikowski, Artur & Hofman, Sebastian, 2021, Crenobiont, stygophile and stygobiont molluscs in the hydrographic area of the Trebisnjica River Basin, ZooKeys 1047, pp. 61-89 : 61

Ancylus sp.

Fig. 14L, M

GenBank no.

COI: MW879254-MW879255

Remarks.

Considering the shell morphology, it should be determined as A. fluviatilis O. F. Müller, 1774, a species reported from this region. However, Pfenninger et al. (2003) demonstrated that A. fluviatilis inhabits a wide range throughout Europe, but in the southern regions there are a few cryptic, molecularly defined species of Ancylus . Our Ancylus sp. molecularly belonged to the Clade 4 of Pfenninger et al. (2003) and " Ancylus sp. C4" of Albrecht et al. (2006) (Fig. 15 ). It was found as a crenobiont in the cave springs at the localities 8, 9 and 16 (Fig. 4 ). Molecular divergence between this Ancylus sp. and Ancylus recurvus is 7%, and similar value (7.5%) is observed between this Ancylus sp. and A. fluviatilis .

Figures

Figure 4. Distribution of the studied taxa. Localities' numbers correspond to Table 1.

Figure 14. Shells of the studied gastropods: A-C Emmericia ventricosa (localities: A - 1, B - 5, C - 12) D, E Paladilhiopsis arion (locality 21) F Valvata montenegrina (locality 20) G Radix labiata (locality 16) H, I Galba truncatula (localities: H - 15, I - 16) J, K Ancylus recurvus (localities: J - 15, K - 13) L, M Ancylus sp. C 4 (localities: L - 9, M - 16) N Succinea cf. putris (locality 10). Scale bars: 1 mm.

Figure 15. Molecular relationships of the studied Ancylus based on COI; our sequences in red and orange, the other from GenBank; bootstrap supports given if over 60 %, their values together with Bayesian probabilities.