Genomic analysis reveals new species and subspecies of butterflies Zhang, Jing Cong, Qian Shen, Jinhui Song, Leina Grishin, Nick V. The Taxonomic Report of the International Lepidoptera Survey 2023 2023-12-13 11 6 1 63 Zhang & Cong & Shen & Song & Grishin 2023 [711,836,536,569] 24 24 subGenus subgen. nov. Grishin    Typespecies.  Themone poecilaH. Bates, 1868.  Definition.As shown above,  Themone poecilaH. Bates, 1868( typelocality Brazil: Amazonas, Ega [= Tefé]) belongs to the genus  IsapisE. Doubleday, 1847( typespecies  Papilio agyrtusCramer, 1777) and not to  ThemoneWestwood, 1851( typespecies  Helicopis paisHübner, [1820]) ( Fig. 21). However, it is genetically differentiated from the typespecies of  Isapisat the subgenus level, e.g., their COI barcodes differ by 8.1% (53 bp) and is sister to the clade of two subgenera:  Isapisand  Callitera  subgen. n.Therefore, we propose that the lineage with  Isapis poecilarepresents a new subgenus. This subgenus differs from its relatives by a combination of the following characters: each wing is blackish-brown with a yellow-orange area towards the base and a central yellow spot, which may be vestigial on the dorsal side. In DNA, a combination of the following characters is diagnostic in nuclear genome: cne792.14.1:C927T, cne792.14.1:A132T, cne 2411.1.1:A348T, cne5004.10.6:A822T, cne5004.10.6:G633A, cne5335.1.1:C114C (not T), cne5335.1.1:T129T (not C), cne5331.3.1:A53A (not G), cne5331.3.1:T237T (not C), cne573.8.1: C63C (not G) and in COI barcode: A4C, C81T, T88A, A278A, 421C, A586A.   Etymology.The name of the typespecies,  poecila, typically refers to colorful or variegated markings or patterns. It is derived from the Greek word ποικίλος (poikilos), which means varied, diverse, or multicolored. The name of the new subgenus is formed from the Spanish word matizado, which means variegated or mottled. The name is treated as a feminine noun in the nominative singular.  Species included.Only the typespecies.  Parent taxon.Genus  IsapisE. Doubleday, 1847.