Four new species of Sabicea (Rubiaceae) from tropical Africa, with additional notes on the genus Zemagho, Lise Lachenaud, Olivier Sonké, Bonaventure Candollea 2018 2018-11-23 73 2 277 293 8Y97Y Zemagho, O. Lachenaud & Sonke Zemagho, O. Lachenaud & Sonke 2018 [801,1334,918,943] Magnoliopsida Rubiaceae Sabicea Plantae Gentianales 1 278 Tracheophyta species desseinii sp. nov.     Holotypus: GABON.  Ogooué-Ivindo: Peripheric area (NE) of Mwagna National Park, close to Mabekwe camp, 0°35'49"N 13°49'57"E,  506 m,  19.I.2018, fl. & fr.,  Texier et al.1895( MO!;  isotype: BR!, BRLU!, LBV, P, WAG!).   Inflorescentiis capitatis ad nodos defoliatos saepe natis, calyce tubo conspicuo sed lobis longioribus, stipulis reflexis, ramulis foliisque indumento hirsuto Sabicea geophiloide Wernham, S. pilosae Hiernet S. speciosae K. Schum.similis. A S. geophiloide differtcalycis tubo intus dense villoso (nec glabro), corollae tubo 22‒27 mmlongo (nec 14‒20 mm) et extus longe villoso (nec pilis appressis brevibus munito), ramis foliisque indumento densiore et in sicco rufescente (nec albido). A S. pilosa calycislobis anguste lanceolatis 0.7‒1.5 mmlatis intus villosis et statu fructifero patentibus (nec ovatis, 1.8‒5 mmlatis, intus glabris et statu fructifero erectis), a S. speciosa corollaalba (nec rubra), bracteis rubris vel rubrescentibus (nec viridibus), calycis lobis pilis longioribus 1‒2.5 mm(nec 0.5‒ 1 mm) munitis, et ab ambabus inflorescentiis 6‒15-floris (nec 1‒5-floris) bracteis 4 (nec 2) cinctis bracteolisque carentibus distinguitur. Twining woody liana, up to 3 mtall; young stems cylindrical, 1‒3 mmthick, hirsute, with stiff hairs 1‒3 mmlong, often intermixed with much shorter uncinate hairs; older stems 4-sulcate, the bark peeling in strips. Stipulesinterpetiolar, ovate, 6‒ 20 × 3.5‒8 mm, acute to obtuse at apex, recurved backwards, hirsute outside, glabrous inside except at the base, persistent. Leavesopposite, equal; petiole 0.5‒4 cmlong, with same indumentum as the stems; leaf-blade elliptic to obovate, 5‒13.5 × 2.6‒6.2 cm, cuneate to rounded at base, acuminate at apex, papyraceous, green on both sides but markedly paler below, drying olive green to olive brown; both surfaces hirsute, with hairs 0.5‒ 1.2 mmlong, denser below; secondary veins 7‒12 pairs, markedly curved and ascending, eucamptodromous; tertiary veins darker and conspicuous below, forming a dense reticulum with areolae c. 1 mmin diameter. Inflorescencesusually on old leafless stems, more rarely axillary, solitary, capitate and involucrate, 6–15-flowered; peduncle 0.2–0.5 cmlong, hirsute; involucre consisting of two pairs of free bracts, these red to greenish-red, ovate, 6.5‒ 13 × 5‒ 8.5 mm, acute at apex, the inner pair often narrower than the outer, hirsute outside, glabrous inside except at the base, persistent. Flowers5-merous, sessile, heterostylous. Calyxred to greenish-red; tube 2‒3 × 2‒3 mmat anthesis, accrescent to 5‒7 mmlong in the fruiting stage, hirsute to densely appressed-pubescent outside, densely villose with appressed hairs inside; lobes lanceolate, 10 ‒15 × 0.7‒ 1.5 mm, acute at apex, ± erect at anthesis and becoming patent afterwards, hirsute with stiff hairs 1‒2.5 mmlong on the margin (and sometimes more sparsely on both surfaces) and shorter appressed to half-erect hairs 0.5‒1 mmlong inside (sometimes also outside) and a basal area inside with very dense appressed hairs in continuation of the tube. Corollawhite; tube 22‒27 mmlong × 1‒1.5 mmwide at base and 2‒3 mmwide at apex, almost cylindrical, villose outside (except at the base) with patent to appressed long silky hairs 1 ‒1.5 mmlong often intermixed with shorter crisped hairs, sparsely pubescent inside except at the very base; lobes triangular, 2‒ 3 × 1.2 mm, outside with same indumentum as the tube, inside glabrous. Stamensincluded for their whole length, inserted around the upper 1/3rd of the corolla tube (long-styled flowers) or included except for the tip, attached a little below throat (short-styled flowers), subsessile; anthers c. 4 × 0.4 mm. Ovary 1.5‒2 mmlong, 5-locular, densely villose with stiff appressed to ± patent hairs 1 ‒ 2 mmlong. Diskcylindrical, c. 0.3 mmlong, glabrous. Style5-lobed, exserted, c. 29 mmlong (long-styled flowers) or included, c. 15 mmlong (short-styled flowers), 5-lobed at the apex, the stigmas linear, 5‒ 5.5 mmlong, papillose, otherwise glabrous. Fruitsbright red, ellipsoid, 11.5‒14 × 8.5‒13 mmwhen dry, hirsute to appressed-pubescent, crowned with persistent calyx, on pedicels 1‒2.5 mmlong. Seedspolygonal, pale brown, 0.5‒0.7 mmlong, the surface with close parallel striations.   Etymology. – Named after Dr. Steven Dessein, the Director of the Botanic Garden Meise, in recognition of his active support to the authors.   Distribution, ecologyand phenology. – Lower Guineasubcentre of endemism (WHITE, 1979). This species occurs in southern Cameroon, Equatorial Guinea( Rio Muni) and north-eastern Gabon( Fig. 4); it may be expected in adjacent Republic of Congo. It occurs in secondary rainforest, up to 530 min altitude, sometimes on seasonally flooded soils, and is locally common in Cameroon (J. & A. Raynal 9859)but apparently rare elsewhere. Flowers have been collected in October, November and January; fruits in January, February and July.   Conservation status. – The extent of occurrence (EOO) of  S. desseiniiis estimated to be 33,344 km² (exceeding the limit for “Vulnerable” status under criterion B1) and its area of occupancy (AOO) to be 28 km² (within the limit for “Endangered” under criterion B2). The species is restricted to Atlantic Central Africa and is known from eight specimensrepresenting seven subpopulations. One of these occurs in a protected area, the Monte Alén National Park in Equatorial Guinea; the other subpopulations are unprotected and exposed to deforestation for agriculture and mining. Even though the species occurs in secondary forest, and thus tolerates a certain degree of habitat degradation, the level of human pressure in its range leads us to anticipate a decline in the extent and quality of habitat, number of locations, number of individuals, and thus EOO and AOO. The seven subpopulations represent seven “locations” (sensu IUCN, 2012) and the species qualifies for “Vulnerable” status [VU B2ab(i,ii,iii,iv,v)] using the IUCN Red List Categories and Criteria ( IUCN, 2012).   Notes. –  Sabicea desseiniisuperficially resembles  S. speciosaK. Schum., but is probably more closely related to  S. geophiloidesWernhamand/or  S. pilosaHiern. Allthese species have in common (sub)capitate inflorescences that are often borne on older stems below the leaves, a calyx tube well developed but shorter than the lobes, a relatively long corolla tube, reflexed stipules, and a hirsute indumentum usually covering most of their organs (though some variants of  S. pilosaare almost glabrous). The differences between these four species are summarised in Table 1.  Sabicea desseiniialso resembles  S. gabonica(Hiern) Hepperin its inflorescences and calyces, but the latter species has erect stipules, stems and leaves with appressed pubescence, and inflorescences borne on young leafy stems and usually surrounded by a single pair of bracts, these green in colour. The only specimen from Gabonwas chosen as typesince it is the most complete (with both flowers and fruits) and has numerous duplicates. It differs slightly from the rest of the material (from Cameroonand Equatorial Guinea) by the appressed (vs patent) indumentum of the calyx tube, ovaries and fruits, and the slightly shorter indumentum of the stems, but in other characters all collections agree very well and their conspecificity is beyond doubt. The colour of the bracts and calyces may vary from deep red to pale reddish-green in the same population ( Fig. 1A–B).     Paratypi. –  CAMEROON. South: Station du Cacaoyer de N’koemvone, S. of Ebolowa,  14 kmon the road to Ambam, on bank of the Seng river, 2°49'N 11°08'E,  16.X.1974, fl.,  J.J.F.E. de Wilde7648A( WAG);  près Bidjap, piste pygmée d’Akom II,  75 kmW Ambam,  30.XI.1979, fl.,  Letouzey15288( BR, P, YA);  Mékomengona(  17 kmSW Ambam), au N du village,  20.II.1963, fr., J. & A. Raynal9859( P, YA);  3 Km NE de Bindem, 2º41'27"N 10º47'31"E,  21.VII.2008, fr.,  Sonké& Simo5004( BR).    EQUATORIAL GUINEA: Inselberg de Bicurga, près du village de Bicurga, 1°35'N 10°28'E,  18.II.2001, fr.,  Parmentier1758( BRLU);  ibid. loco,  24.V.2002, fl.,  Parmentier& Esono3159( BRLU);  Monte Alén, 1°38'N 10°18'E,  10.VIII.2001, fr.,  Sonké, Esono& Ndong2498( BRLU). 3470697305 2018-01-19 MO Texier et al. Gabon 506 0.59694445 Peripheric area (NE) of Mwagna National Park 21 13.8324995 1 278 1 Ogooue-Ivindo holotype 3470697304 [1001,1375,1088,1114] BR, BRLU, LBV, P, WAG 1 278 1 isotype 3470697330 1974-10-16 WAG J. J. F. E. de Wilde Cameroon 2.8166666 Station du Cacaoyer de N'koemvone 1307 11.133333 South 5 282 1 paratype 3470697333 1979-11-30 BR, P, YA Letouzey pres Bidjap 5 282 1 3470697317 1963-02-20 P, YA A. Raynal au N du village Mekomengona 5 282 1 3470697331 2008-07-21 BR Sonke & Simo 2.6908333 21 10.7919445 5 282 1 3470697338 2001-02-18 BRLU Parmentier Equatorial Guinea 1.5833333 pres du village de Bicurga 1308 10.466666 Inselberg de Bicurga 5 282 1 3470697326 2002-05-24 BRLU Parmentier & Esono 5 282 1 3470697339 2001-08-10 BRLU Sonke & Esono & Ndong 1.6333333 Monte Alen 1308 10.3 Monte Alen 5 282 1