Laboulbeniomycetes (Fungi, Ascomycota) of Denmark Santamaria, Sergi Pedersen, Jan European Journal of Taxonomy 2021 2021-11-26 781 1 425 B2MJ3 Santam. Santamaria & Pedersen 2021 [529,962,390,417] Laboulbeniomycetes Laboulbeniaceae Monoicomyces Fungi Laboulbeniales 336 337 Ascomycota species crassicaulis sp. nov.  MB#840607  Figs 84, 107B    Diagnosis Similar to  M. fragilis, but differing by an enlarged, inflated cell VI, by a more elongated perithecia, with septa between wall cell tiers marked by elevations, and by a narrow, bent perithecial neck.    Etymology The species epithet derives from  crassus, “fat”, and caulis, “stalk”, referring to the inflated perithecial stalk cell.    Type material    Holotype DENMARK–  Nordvestsjaelland(NWZ)• Garbølle; 55°34.107′ N, 11°34.293′ E; PG66; on  Oxypoda elongatulaAubé, 1850(Col. Staphylinidae Aleocharinae);  21 Apr. 2018;  JP1059; JPdet.; ZMUC C-F-123576.    Paratypes DENMARK–  Østjylland(EJ)• Skallerundi Stenholt Skov; 56°11.410′ N, 9°20.787′ E; NH22; on  O. elongatula;  24 Mar. 2019;  L. Kjaer-Thomsen& L. Thomas1401; JPdet.; ZMUC C-F-123947•  Vestfor Ørnsøved Silkeborg; 56°9.126′ N, 9°30.588′ E; NH32; on  O. elongatula;  24 Nov. 2019;  JP1545; JPdet.; ZMUC C-F-124306. –   Nordøstsjaelland(NEZ)• Skodsborg Dami Jaegersborg Hegn; 55°49.068′ N, 12°33.937′ E; UB48; on  O. elongatula;  18 Nov. 2018;  JP1244; JPdet.; ZMUC C-F-123772•  Sydfor Løjesøi Rude Skov; 55°49.866′ N, 12°28.551′ E; UB49; on  O. elongatula;  12 Apr. 2018;  JP1034; JPdet.; ZMUC C-F-123546. –   Sydsjaelland(SZ)• Denderup Søi Denderup Vaenge; 55°15.075′ N, 11°57.366′ E; PG82; on  O. elongatula;  9 Apr. 2019;  JP1425; JPdet.; ZMUC C-F-123971•  Holmegårds Mose; 55°17.685′ N, 11°47.928′ E; PG73; on  O. elongatula;  31 Mar. 2019;  JP1411; JPdet.; ZMUC C-F-123957•  Storskovsyd for Sparresholm; 55°13.234′ N, 11°57.463′ E; PG82; on  O. elongatula;  17 Feb. 2019;  JP1389; JPdet.; ZMUC C-F-123932•  Tryggevaelde Å ved Varpelev; 55°21.055′ N, 12°15.877′ E; UB23; on  O. elongatula;  6 Jan. 2018;  JP1592; JPdet.; ZMUC C-F-124353. –   Vestjylland(WJ)• Sønderkaersyd for Bindeballe; 55°39.107′ N, 9°16.682′ E; NG16; on  O. elongatula;  10 Mar. 2002;  H. Liljehult1469; JPdet.; ZMUC C-F-124049•  Velling; 56°3.078′ N, 8°18.596′ E; MH51; on  O. elongatula;  16 Dec. 2018;  JP1266; JPdet.; ZMUC C-F-123794.    Description Thallus amber to brown, with darker antheridia, appendages, and foot. Basal cell of receptacle (I) obconical, about 1.5 times as long as broad. Suprabasal cell of receptacle (II) small, trapezoidal, broader than long. Cell III entirely darkened, with the inner side distinctly convex ( Fig. 84G–H). Each thallus includes two to several secondary receptacles arising from cell II, with respective perithecia and antheridia. Primary appendage unbranched, very elongated if unbroken, entirely brown, consisting of a short, inflated basal cell, and several very elongated cells, exceeding in length the perithecial apices. Basal cell of primary appendage between two constricted and darkened septa above and below; the latter is the primary septum ( Fig. 84G–H, a).   Fig. 84.  Monoicomyces crassicaulisSantam. sp. nov. A–C. Mature thalli. In A, perithecial wall cell tiers (w 1–4) have been labelled. D–F. Respectively, near, middle and far focus of antheridium in detail. In E, the four phialides have been labelled (an). In F, the four tiers have been labelled (t1–4). G–H. Lower receptacle (I, II, and III) and primary appendage above the primary septum (a) in detail. Scale bars:A–C = 50 µm; D–H = 20 µm. Photographs from slides ZMUC C-F-123576 (A) (holotype), ZMUC C-F-123971 (B), ZMUC C-F-123794 (C–H). Secondary receptacles including an externally darkened stalk supporting perithecia and antheridia with secondary appendages. Antheridia consisting of four superposed tiers of paired cells ( Fig. 84D–F, tn). Each cell in the first tier is about three times as long as broad and fully dark brown. Brown suffusion occupies the basal area of 2 ndtier, with cells about twice longer than broad. Cells of 3 rdtier hyaline and subtriangular. The 2 ndand 3 rdtiers of cells bear four phialides altogether ( Fig. 84E, an). The 4 thtier consists of two subtriangular cells, smaller than cells of the 3 rdtier; bearing two dark, very elongated secondary appendages which exceed in length the perithecial apices, and sometimes two cells representing initials for an additional pair of secondary appendages ( Fig. 84D, *). Perithecial stalk cell (VI) 3–4 times as long as broad, narrower and darkened at the base, distinctly inflated upwards, then abruptly constricted below the perithecial base. Perithecia elongate fusiform, with septa separating the two lower tiers of wall cells marked by slight elevations; broader at w 1-w 2level which form a deeply pigmented venter ( Fig. 84A). Tiers w 3and w 4almost hyaline, the w 3forming a narrow neck which slightly bents laterally and ends in a blunt apex ( Fig. 84A). Maximum length from foot to tip of perithecium 175–242 µm. Perithecium (including basal cells) 115– 133 × 27–34 µm. Antheridium (including pedicellar cell, without secondary appendages) 54–62 × 12– 16 µm. Length of primary appendage (when undamaged) from primary septum above cell III 209–294 µm.  Thalli were found on various body parts of the hosts. The hosts were sifted from moist moss and leaf litter in a partly shady peat bog, beaten from rush in a sun-exposed swamp along a rivulet, sifted from moist beech leaf litter in a partly dried out forest pond, sifted from wet  Sphagnummosses and leaves around  Carextussocks in a sun-exposed  Sphagnumbog, sifted from moist fine crumb and mice-nests in and under  Carex paniculatatussocks at the edge of a sun-exposed forest pond, sifted from moist leaf litter and moss in a raised bog, sifted from moist moss and leaf litter in a partly sun-exposed alder swamp, sifted from wet, fresh flood debris along the banks of a river and sifted from moist leaf litter and moss in an open willow/birch/alder swamp.   Remarks  Majewski (1994b)already mentioned that thalli on  Oxypodadiffer from those on  Ocaleaalthough he did not formally describe any new taxa. After studying the abundant material from Danish  Oxypoda elongatula, we conclude that a new species should be erected because the differences are stable and remarkable.As mentioned above, in comparison with the closely allied  M. fragilis, thalli of  M. crassicaulis sp. nov.are larger, with narrower perithecia having a well-distinguished, bent neck, with a simple primary appendage, with both cells III as well as the basal cell of appendage producing a very characteristic figure, in addition to a broadened and distally inflated cell VI. 4988641240 on Oxypoda elongatula Aube, 1850 2018-04-21 ZMUC JP Denmark JP 55.56845 Garbolle 1 11.57155 Nordvestsjaelland 336 337 ZMUC C-F- 123576 1 holotype 4988641933 on O. elongatula 2019-03-24 ZMUC L. Kjaer-Thomsen & L. Thomas Denmark JP 56.190166 Stenholt Skov 1 9.34645 Skallerund 336 337 ZMUC C-F-123947 1 Ostjylland paratype 4988641774 on O. elongatula 2019-11-24 ZMUC JP Denmark Vest JP 56.1521 Silkeborg 1 9.5098 Ornso 336 337 ZMUC C-F-124306 1 Ostjylland paratype 4988640476 on O. elongatula 2018-11-18 ZMUC JP Denmark JP 55.8178 Jaegersborg Hegn 1 12.565617 Skodsborg Dam 336 337 ZMUC C-F-123772 1 Nordostsjaelland paratype 4988641768 on O. elongatula 2018-04-12 ZMUC JP Denmark Syd JP 55.8311 Rude Skov 1 12.47585 Lojeso 336 337 ZMUC C-F-123546 1 Nordostsjaelland paratype 4988642086 on O. elongatula 2019-04-09 ZMUC JP Denmark JP 55.25125 Denderup Vaenge 1 11.9561 Denderup So 336 337 ZMUC C-F-123971 1 Sydsjaelland paratype 4988641678 on O. elongatula 2019-03-31 ZMUC JP Denmark JP 55.29475 Holmegards Mose 1 11.7988 336 337 ZMUC C-F- 123957 1 Sydsjaelland paratype 4988640511 on O. elongatula 2019-02-17 ZMUC JP Denmark JP 55.220566 Sparresholm 1 11.957717 Storskov 336 337 ZMUC C-F-123932 1 Sydsjaelland paratype 4988640588 on O. elongatula 2018-01-06 ZMUC JP Denmark JP 55.350918 Tryggevaelde A ved Varpelev 1 12.264617 336 337 ZMUC C-F-124353 1 Sydsjaelland paratype 4988642206 on O. elongatula 2002-03-10 ZMUC H. Liljehult Denmark JP 55.651783 Bindeballe 1 9.278033 Sonderkaer 336 337 ZMUC C-F-124049 1 Vestjylland paratype 4988641246 on O. elongatula 2018-12-16 ZMUC JP Denmark JP 56.0513 Velling 1 8.309934 336 337 ZMUC C-F-123794 1 Vestjylland paratype