Radix Scutellariae

Liu, Wei, Wang, Huilin, Zhu, Bo, Yin, Chengqian, Chen, Shuyang, Li, Jin, Azietaku, John Teye, An, Mingrui, Gao, Xiu-mei & Chang, Yan-xu, 2017, An activity-integrated strategy of the identification, screening and determination of potential neuraminidase inhibitors from Radix Scutellariae, PLoS ONE (e 0175751) 12 (5), pp. 1-19 : 13

Quantitative analysis of the different types of Radix Scutellariae by 26 NA inhibitory activity compounds

To determine the contents of each bioactive component in NA inhibitory of 18 RS samples,

the UPLC-DAD was used. There are 18 RS samples which include three group of 1. biennially cultivated RS, 2. perennially (3 years or more) cultivated RS and 3. wild RS. The detailed information of the 18 RS samples was summarized in Table 1. Concentrations of the 26 components in 18 RS samples were shown in Table 6. Seven of active components were quantified with standards, including scutellarin, baicalin, chrysin-7-O-glucuronide, wogonoside. baicalein, wogonin and chrysin. Specifically, the corresponding concentration were 0.44–0.54 μg

mg -1, 244.23–421.18 μg mg -1, 4.72–10.32 μg mg -1, 52.91–104.89 μg mg -1, 8.21–32.23 μg mg -1

and 1.60–2.73 mg mg −1, respectively. The other 19 components were semi-quantitatively determined by the calibration curve of scutellarin due to lacking the corresponding standard.

The flavones (baicalin and wogonoside) are the most abundant component in RS extract among all the 18 samples with an average concentration of 116.54 mg g -1. It is also found that the concentration of flavones (baicalin and wogonoside) in perennially (3 years or more) cultivated RS was significantly higher than that in biennially cultivated RS. Baicalin is the major component in total flavones, which reaches up to more than 5% of the gross total weight of RS

sample Table 6, So that baicalin was expected to serve as the major contributor to NA inhibitory activity of RS extract.