Toxoplasma gondii subsp. detection

Kurth, Katherine, Jiang, Tiantian, Muller, Lisa, Su, Chunlei & Gerhold, Richard W., 2021, Toxoplasma gondii contamination at an animal agriculture facility: Environmental, agricultural animal, and wildlife contamination indicator evaluation, International Journal for Parasitology: Parasites and Wildlife 16, pp. 191-198 : 193

3.1. Soil T. gondii detection

We were able to detect H. hammondi from both soil samples spiked with oocysts, indicating the methodology was effective at that concentration. However, the results of PCR-RFLP detection of T. gondii oocyst were not consistently positive ( Table 1). In the first test, T. gondii appeared in the positive controls (PTG strain) and in two soil samples. In the repeat experiment, an additional soil sample was T. gondii positive, and earlier positive samples were not consistently positive ( Table 1). When testing if the addition of five more grams of soil from each of the 22 samples increased oocyst detection, no positive samples were detected ( Table 2).

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