Trichosetodes ratanakiriensis Laudee & Malicky, 2023

Laudee, Pongsak, Malicky, Hans, Kong, Chamroeun, Takenaka, Masaki & Tojo, Koji, 2023, New species of the genus Trichosetodes Ulmer, 1915 (Trichoptera, Leptoceridae) from Ratanakiri province, Cambodia, based on morphological and molecular data, ZooKeys 1182, pp. 153-164 : 153

Trichosetodes ratanakiriensis Laudee & Malicky sp. nov.

Fig. 3

Type material.

Holotype. Male. Cambodia: Ratanakiri Province: Banlung, Katieng Waterfall, 13°40'38"N, 106°58'33"E, elev. 203 m, 13.v.2022, Pongsak Laudee. (CHM). Paratypes: same data as holotype. 33 males: 18 males (PSUNHM), 5 males (CHM), 5 males (CUAC), 5 males (NMPC).

Diagnosis.

The male genitalia of T. ratanakiriensis are moderately similar to those of T. pandareos Malicky, 2006 described in Laos and T. kampongspeuensis Malicky & Kong, 2020 (Fig. 4 ), however it can be differentiated by the shape of left inferior appendage and the shape of segment IX. The left inferior appendage is forked in T. pandareos whereas this feature is missing in T. ratanakiriensis . In addition, the prolongations of the subapicoventral part of segment IX are symmetric in T. pandareos but asymmetric in T. ratanakiriensis in ventral view. Compared to T. kampongspeuensis , T. ratanakiriensis exhibits a longer, cylindrical right inferior appendage with a sharp apex in lateral view, whereas in T. kampongspeuensis , this appendage is oval and splits into two at the tip. The prolongations of subapical part of segment IX in ventral view are thin in T. kampongspeuensis but prominent in T. ratanakiriensis .

Description.

Length of each male forewing 5 mm (N = 12); specimens in alcohol with head, thorax, abdomen, legs, forewings dark brown.

Male genitalia (Fig. 3A-D ). Segment IX square with U-shaped incision anteriorly in dorsal view (Fig. 3C ); in left lateral view, complicated shape, strongly convex anteriorly, undulated edge dorsally, curved downward ventrally with pimple sub-anteroventrally, long cylindrical subapicoventrally, rounded apically (Fig. 3A ); in right lateral view the same as left lateral view but without pimple (Fig. 3B ); in ventral view, vertical profile rectangular with 1/3 of height in U-shaped incision apically, rounded apex (Fig. 3D ). Preanal appendages slender covered with hairs (Fig. 3A, B ). Segment X not evident. Inferior appendages asymmetrical; circular basally, conical apically in left lateral view (Fig. 3A ); in right lateral view, cylindrical with expanded basally, curved downward with tooth at dorsal edge subapically, pointed apically (Fig. 3B ); in dorsal and ventral view, left inferior appendage claw-like, right inferior appendage long claw-like with an inner tooth. Phallicata large, tubular, curved and bent subapically, pointed apically in dorsal view; in lateral view, upside down U-shaped, pointed apically.

Etymology.

The species is named for the type locality, Ratanakiri Province.

Molecular analysis

The molecular diversity of the new Trichosetodes species was analyzed using the mitochondrial large subunit ribosomal rRNA gene region (16S rRNA). Based on genetic distance (p -distance) of this gene fragment, T. ratanakiriensis sp. nov. and T. kampongspeuensis have a close genetic relationship, whereas T. carmelae sp. nov. showed greater genetic divergence from both T. ratanakiriensis sp. nov. and T. kampongspeuensis (Table 1 ). The estimated phylogenetic relationships based on the mtDNA 16S rRNA are shown in Fig. 5 , where all three Trichosetodes sp. nov. were genetically differentiated. The monophyly of T. ratanakiriensis sp. nov. and T. kampongspeuensis was highly supported by the bootstrap value (NJ BP). In addition, T. carmelae sp. nov. was differentiated from the monophyletic clade of T. ratanakiriensis sp. nov. and T. kampongspeuensis (Fig. 5 ).

Figures

Figure 3. Trichosetodes ratanakiriensis sp. nov. male genitalia A segment IX and inferior appendages, left lateral B segment IX and inferior appendages, right lateral C segment IX and phallus, dorsal D segment IX, ventral. Abbreviations: Le Inf = left inferior appendages, Ri Inf = right inferior appendage, Su Api = subspicoventral part of segment IX.

Figure 4. Trichosetodes kampongspeuensis male genitalia A segment IX and inferior appendages, left lateral B segment IX and inferior appendages, right lateral C segment IX and phallus, dorsal D segment IX, ventral.

Figure 5. Estimated phylogenetic relationships using the Neighbor-Joining clustering method for Trichosetodes spp. based on the mtDNA 16 S rRNA region. Sequences of Setodes brevicaudatus were included as outgroups. Each node's bootstrap value is shown (based on 1000 replicates). The scale bar indicates genetic distance (p - distance).