Azospirillum thiophilum WD

Han, Ji Yeon, Lim, Oung Bin, Chea, So-Yi, Lee, Hyosun, Lee, Ki-Eun, Cha, In-Tae & Kim, Won-Jae Chi and Dong-Uk, 2022, A report on 20 unrecorded bacterial species of Korea isolated from soil in 2021, Journal of Species Research 11 (4), pp. 310-320 : 318

Description of Azospirillum thiophilum WD 4

Cells are Gram-stain-negative and rod-shaped. Cell size is 1.4-1.6 μm. Colonies are irregular, smooth, entire, and white pigmented after 3 days of incubation at 28°C on R2A. Positive for reduction of nitrates to nitrite (NO 3 ->NO 2 -), urease, β -glucosidase (esculin hydrolysis), β -Galactosidase (PNPG), D-glucose, L-arabinose, D-mannitol, gluconate, caprate, malate, and phenyl-acetate; but negative for indole production, glucose acidification, arginine dihydrolase, protease (gelatin hydrolysis), D-mannose, N -acetyl-D-glucosamine, D-maltose, adipate, and citrate. Utilizes D-mannitol, D-glucose, D-sorbitol, L-arabinose, propionate, caprate, valerate, L-histidine, 2-ketogluconate, 3-hydroxy-butyrate, 4-hydroxy-ben- zoate, L-proline, D-ribose, malonate, acetate, lactate, and L-alanine; but does not utilize salicin, D-melibiose, L-fucose, citrate, L-rhamnose, N -acetyl-D-glucosamine, inositol, D-sucrose, D-maltose, itaconate, suberate, 5-ke- togluconate, glycogen, 3-hydroxy-benzoate, and L-serine. Enzymatic activity reaction was positive for alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, acid phosphatase, naphtol-AS-BI-phosphohydrolase, β -galactosidase, and β -glucosidase; but negative for lipase (C14), valine arylamidase, cystine arylamidase, trypsin, α -chymotrypsin, α -galactosidase, β -glucuronidase, α -glucosidase, N -acetyl- β -glucosamin- idase, α -mannosidase, and α -fucosidase. Strain WD4 (= NIBRBAC000509140) was isolated from an agricultural soil sample, Chungcheongnam-do, Korea.