Corynebacterium glutamicum RB

Han, Ji Yeon, Lim, Oung Bin, Chea, So-Yi, Lee, Hyosun, Lee, Ki-Eun, Cha, In-Tae & Kim, Won-Jae Chi and Dong-Uk, 2022, A report on 20 unrecorded bacterial species of Korea isolated from soil in 2021, Journal of Species Research 11 (4), pp. 310-320 : 317

Description of Corynebacterium glutamicum RB 28

Cells are Gram-stain-positive and rod-shaped. Cell size is 1.1-1.3 μm. Colonies are circular, smooth, entire, and yellow pigmented after 3 days of incubation at 28°C on R2A. Positive for reduction of nitrates to nitrite (NO - 3> NO 2 -), glucose acidification, urease, D-glucose, D-mannose, D-maltose, gluconate, and citrate; but negative for indole production, arginine dihydrolase, β -glu- cosidase (esculin hydrolysis), protease (gelatin hydrolysis), β -Galactosidase (PNPG), L-arabinose, D-mannitol, N -acetyl-D-glucosamine, caprate, adipate, malate, and phenyl-acetate. Utilizes D-glucose, propionate, citrate, 4-hydroxy-benzoate, D-ribose, inositol, D-sucrose, D-maltose, malonate, acetate, lactate, and 3-hydroxy-ben- zoate; but does not utilize D-mannitol, salicin, D-melibiose, L-fucose, D-sorbitol, L-arabinose, caprate, valerate, L-histidine, 2-ketogluconate, 3-hydroxy-butyrate, L-proline, L-rhamnose, N -acetyl-D-glucosamine, itaconate, suberate, L-alanine, 5-ketogluconate, glycogen, and L-serine. Enzymatic activity reaction was positive for esterase (C4), leucine arylamidase, acid phosphatase, and naphtol-AS-BI-phosphohydrolase; but negative for alkaline phosphatase, esterase lipase (C8), lipase (C14), valine arylamidase, cystine arylamidase, trypsin, α -chymotryp- sin, α -galactosidase, β -galactosidase, β -glucuronidase, α -glucosidase, β -glucosidase, N -acetyl- β -glucosamin- idase, α -mannosidase, and α -fucosidase. Strain RB28 (= NIBRBAC000509138) was isolated from an agricultural soil sample, Gyeongsangnam-do, Korea.