Steinernema diaprepesi (Nguyen and Duncan, 2002) AM

B, Samanta Letícia Lopes Nanzer, B, Gustavo Henrique Recchia, B, Julie Giovanna Chacon-Orozco, B, Raphael Satochi Abe Silva, B, Jorge Franco Maringoli Cardoso & Leite, Luis Garrigós, 2021, Assessment of entomopathogenic nematodes and their symbiotic bacteria to control the stink bugs Euschistus heros and Dichelops melacanthus (Heteroptera: Pentatomidae) in the soybean-corn succession system, Turkish Journal of Zoology 45, pp. 356-371 : 359

2.6. Virulence of Steinernema diaprepesi at different concentrations against Euschistus heros

This experiment assessed different concentrations of Steinernema diaprepesi ( Nguyen and Duncan, 2002) AM 163 on the mortality of E. heros adults. Five treatments consisted of the concentrations 0.7, 3.5, 17.6, 88.4 and 442.4 IJs/cm² (≅1, 40, 200, 1000, 5000 IJs/insect), considering the area on the substrate surface (113 cm ²) in the 1.5 L plastic pot (12.5 cm Ø). Each treatment had 4 replications, each replication represented by a 1.5 L plastic pot (12.5 cm Ø) containing 100g of sand (14% humidity), two bean pods, plus ten adult insects. The nematode was applied with a pipette, generating the different rates. The control treatment received only water. The pots were sealed with sheer fabric and elastic bands, and incubated at 25 °C, RH 60 ± 5% and 12-h photoperiod. Evaluation was carried out seven days later, based on insect mortality. The dead insects were transferred to White traps, using a trap for each replication in order to determine the number of IJs generated per insect. The experiment was conducted twice in time.