Thienemanniella ubatuba, Wiedenbrug, Sofia, Lamas, Carlos E. & Trivinho-Strixino, Susana, 2013

Wiedenbrug, Sofia, Lamas, Carlos E. & Trivinho-Strixino, Susana, 2013, A review of Neotropical species in Thienemanniella Kieffer (Diptera, Chironomidae), Zootaxa 3670 (2), pp. 215-237 : 231-234

Thienemanniella ubatuba sp. n.

( Figs 9–11 )

Type material. Holotype male with pupal and larval exuviae; BRAZIL, SP, Ubatuba , Sertão da Quina, Cachoeira da Renata, bridge, 23°31.231´S, 45°14.625´W, 33 m a.s.l., 24.I.2011, S. Wiedenbrug. Paratypes: allotype female with pupal and larval exuviae; same data as holotype, except 8.I.2011, UFSCar SW070. Two females with pupal and larval exuviae; same data as holotype, except one of them UFSCar SW071. One pharate male and three females, all with pupal and larval exuviae, two of them UFSCar 0 66 and 0 68, and one pharate female, UFSCar SW065, all same data as holotype, except 21.I.2011. One male with pupal and larval exuviae, UFSCar SW069, same data as holotype, except 29.XII.2010.

Additional material examined. Two males, two pharate males and one pharate female, all with pupal exuviae, and three pupal exuviae; PR, São Luis do Purunã, encontro das águas, 25°27.099´S, 48°43.459´W, 10.I.2010, S. Wiedenbrug. Four pupal exuviae; MG, Gonçalves, 22°38´37.50´´S, 45°51´27.40´´W, waterfall “Cachoeira das Andorinhas”, 14.I.2009, S. Wiedenbrug. Three pupal exuviae; MG, Paraisópolis, 22°39´54.81´´S, 45°55´38.28´´W, 2nd-order stream, 1.370 m a.s.l., 11.X.2009, S. Wiedenbrug.

Diagnostic characters. The pupa of T. ubatuba is distinguished from other members of the group by sternite I with very fine shagreen, tergite VIII with shagreen with elongate median and posterior spinules, male sternite VIII with median short spines and posterior spinules stronger but not wide-based. The male of T. ubatuba can be distinguished from T. liae Paggi by abdominal tergite color, TII and III being whitish in T. ubatuba , brown in T. liae . The absence of oral projections on the transverse sternapodeme can help to separate T. ubatuba and T. sanctivincenta from T. sancticaroli . The male of T. ubatuba cannot be distinguished from T. spreta . The larva is very similar to T. sancticaroli , but the antenna/postmentum length ratio about 1.0 is diagnostic for T. ubatuba .

Male (n = 1–3). Total length 1.21–1.60 mm. Wing length 0.63–0.76 mm.

Color. Thorax brownish. Tergite I whitish with brownish oral borders; TII and III whitish with brownish median area; TIV–IX brown; TVI and TVII with a white posterior rectangle.

Head. AR = 0.33–0.38. Antenna with 12 flagellomeres, apical flagellomere 100–120 µm long. Flagellomeres with more than one row of setae each. Eyes hairy.

Thorax. Antepronotal lobes tapering, dorsally parallel-sized.

Wing. Clavus/wing length ratio 0.33–0.37. Anal lobe present ( Fig. 9 E).

Legs. Fore and hind trochanter with dorsal keel ( Fig. 9 A, B). Hind tibial scale 5 µm long, with one short spur and one long, curved seta ( Fig. 9 C, D).

Tergites. TI with 2 setae; TIII–VII with 5 setae; TVIII with 2 setae.

Hypopygium ( Fig. 9 F, H). Tergite IX with 6–8 setae. Laterosternite IX with 1 seta. Superior volsella elongated with posterior margin at about 45° angle to median margin. Inferior volsella rounded, positioned apically on gonocoxite margin. Transverse sternapodeme 30–32 µm long, without lateral projections; phallapodeme curved to posterior. Gonostylus with a low crista dorsalis.

For measurements, see Table 1 .

Female (n = 1–2). Wing length 0.57–0.67 mm.

Head. AR = 0.40. Antenna with 5 flagellomeres, apical flagellomere 40 µm long. Flagellomeres with one row of setae each. Eyes hairy.

Wing. Clavus/wing length ratio 0.54. Anal lobe present ( Fig. 10 A). 6 setae at clavus in addition to marginal wing setae (not drawn).

Legs. Hind tibial scale 7 µm long, with one short spur and one long and curved seta. Hind Ta1 with 4–6 sensilla chaetica.

Tergites II–V with 5 setae, VI with 3 or 5, VII with 3, VIII without setae.

Genitalia ( Fig. 10 B, C). Tergite IX with 7 setae. Laterosternite IX with 1 seta. Two subequal seminal capsules, 50 μm long; spermathecal ducts join shortly before seminal eminence. Notum 40 μm long. Apodeme lobe approximately lozenge-shaped, evenly sclerotized, brown. Coxosternapodeme curved. Copulatory bursa with oral median invagination and sclerotized oral borders. Labia membranous, bare. Gonocoxapodeme slightly curved. Cercus 22 μm long.

For measurements, see Table 2 .

Pupa (n = 2–3). Total length 1.45–1.80 mm.

Cephalothorax. Frontal apotome rugose. Thorax suture slightly rugose. Dc-setae in two pairs; Dc1 and Dc3-4 long and taeniate, Dc2 short and hair-like. Wing sheath without pearl rows.

Abdomen ( Fig. 11 A, B). Tergite I bare; TII–V with fine shagreen, with posterior spinules larger; TVI–VIII with homogeneous shagreen, with oral median spinules elongated. Sternite I with very fine shagreen; SII with elongated spinules; SIII–VIII with fine homogeneous shagreen. Tergal conjunctives III/IV–VII/VIII and sternal conjunctives III/IV–VII/VIII with small spinules. Segment I with 1 L-seta, II with 3 and III–VIII with 4 long taeniate L-setae. Anal lobe rounded, with almost complete fringe, 3 taeniate macrosetae, and 1 taeniate median seta. Male genital sac with inner median margin oblique.

For measurements, see Table 3 .

Larva (n = 4). Head. Postmentum 132–140 µm long. Head capsule integument yellow and smooth. Mentum ( Fig. 11 C) with three median teeth, central tooth much smaller than adjacent teeth; first lateral teeth adpressed to outer median teeth. Antenna 135–140 µm long ( Fig. 11 D); segments two and three darker than first segment.

Abdomen. Hair-like setae not modified. Posterior parapod with subbasal seta simple.

For measurements, see Table 4.

Remarks. See remarks on T. sancticaroli sp. n.

Figures

FIGURE 9. Thienemanniella ubatuba sp. n., adult male. A. Fore trochanter. B. Hind trochanter. C – D. Apex of hind tibia in posterior (C) and anterior view (D). E. Wing. F. Hypopygium, general view. G. Hypopygium with tergite IX and gonostyli removed, internal sclerites hatched; left: ventral view, right: dorsal view. H. Gonostylus.

FIGURE 10. Thienemanniella ubatuba sp. n., adult female. A. Wing. B – C. Genitalia. B. Ventral view, C. Dorsal view, also showing coxosternapodemes and labia.

FIGURE 11. Thienemanniella ubatuba sp. n., immature stages. A – B. Pupa. C – D. Larva. A. Abdominal segments I – III, left: dorsal view, right: ventral view. B. Segments VII – IX and anal lobes in dorsal (left) and ventral view (right). C. Mentum. D. Antenna.