Distoseptispora meilingensis Z. J. Zhai & D. M. Hu, 2022

Zhai, Zhi-Jun, Yan, Jun-Qing, Li, Wei-Wu, Gao, Yang, Hu, Hai-Jing, Zhou, Jian-Ping, Song, Hai-Yan & Hu, Dian-Ming, 2022, Three novel species of Distoseptispora (Distoseptisporaceae) isolated from bamboo in Jiangxi Province, China, MycoKeys 88, pp. 35-54 : 35

Distoseptispora meilingensis Z. J. Zhai & D. M. Hu sp. nov.

Fig. 2

Etymology.

Referring to the collecting site of the Meiling Mountain in Jiangxi Province, China.

Holotype.

HFJAU 10009.

Description.

Saprobic on culms of bamboo. Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Colonies effuse, brown to dark brown, hairy. Mycelium mostly immersed, composed of pale to dark brown, septate, branched, smooth, hyaline to subhyaline hyphae. Conidiophores 69-192 × 4-7 μm (x- = 120.6 × 5.5 μm, n = 25), macronematous, mononematous, erect, cylindrical, straight or slightly flexuous, 5-12-septate, yellowish-brown or brown, robust at the base. Conidiogenous cells holoblastic, mono- to polyblastic, integrated, terminal, cylindrical, yellowish-brown or brown. Conidia 32-64.5 × (7-)9-12.5 μm (x- = 43.7 × 9.8 μm, n = 30), acrogenous, solitary, straight or slightly curved, obclavate, 5-7-distoseptate, thick-walled, rounded at the apex, truncate at the base, tapering towards apex, bud scars disjunctors at base, mostly brown when mature.

Cultural characteristics.

Conidia germinating on PDA within 24 h and germ tubes produced from both ends. Colonies on PDA reaching 17-23 mm diam. at two weeks at 25 °C, in natural light, circular, with dense, light olivaceous mycelium on the surface with entire margin; reverse brown to dark brown.

Material examine.

China, Jiangxi Province, Nanchang City, Meiling Mountain , alt. 305 m, near 28.79°N, 115.72°E, on decaying bamboo culms submerged in a freshwater stream, 16 Aug 2021, Z. J. Zhai, SLT-3 ( HFJAU10009, holotype), ex-type living culture, JAUCC 4727 = JAUCC 4728 .

Notes.

Distoseptispora meilingensis clusters with the clade including D. suoluoensis and D. yongxiuensis with high support in the phylogenetic tree (Fig. 1 ). Distoseptispora meilingensis is distinct from D. suoluoensis ( Yang et al. 2018) and D. yongxiuensis by its conidial colour (mostly brown, yellowish-brown to dark olivaceous and yellowish-brown or brown, respectively). Furthermore, D. meilingensis has shorter conidia (32-64.5 μm vs. (65-)80-125(-145) μm) than those of D. suoluoensis ( Yang et al. 2018) and slightly shorter conidiophores (69-192 μm vs. 112-253 μm) than those of D. yongxiuensis . Distoseptispora meilingensis resembles D. bambusae in similar habitats and polyblastic conidiogenous cells ( Sun et al. 2020). However, D. meilingensis can be distinguished from D. bambusae in its longer conidiophores (69-192 μm vs. 40-96 μm), slightly wider (up to 12.5 μm vs. up to 9.5 μm) and brighter (light brown vs. brown) conidia ( Sun et al. 2020). A comparison of morphological features of Distoseptispora species is provided in Table 2 .

Figures

Figure 1. Phylogenetic tree of Distoseptispora, inferred from the combined regions (ITS-LSU-SSU- Tef 1 α) using Bayesian Inference (BI) analysis. The Aquapteridospora clade was used as the outgroup. The lineages with new species were shown in bold. PP ≥ 0.95 and BS ≥ 75 % were indicated around the branches. The new sequences generated in this study are given in bold.

Figure 2. Distoseptispora meilingensis (HFJAU 10009, holotype) a, b colonies on bamboo culms c-e conidiophores with conidia f conidiogenous cells g, n conidiogenous cells with conidia h-m conidia o germinating conidium p culture on PDA from above and reverse. Scale bars: 100 µm (a, b), 20 µm (c-e, o), 5 µm (f-n).