Cadophora luteo-olivacea (J.F.H.Beyma) T.C.Harr. & McNew

Bien, Steffen & Damm, Ulrike, 2020, Arboricolonus simplex gen. et sp. nov. and novelties in Cadophora, Minutiella and Proliferodiscus from Prunus wood in Germany, MycoKeys 63, pp. 119-161 : 119

Cadophora luteo-olivacea (J.F.H.Beyma) T.C.Harr. & McNew Figures 5D , 9

Description.

Sexual morph not observed. Asexual morph on SNA. Vegetative mycelium hyaline, smooth-walled, septate, branched, 1-10 µm wide, hyphal cells often, sometimes very strongly inflated and constricted at the septa, chlamydospores absent. Sporulation abundant, conidia formed on hyphal cells. Conidiophores hyaline, smooth-walled, simple or septate and branched, up to 40 µm long. Conidiogenous cells enteroblastic, hyaline, smooth-walled, cylindrical to ± inflated, 3-14 × 1.5-4 µm, sometimes integrated, necks cylindrical, 0.5-3 µm long, collarettes funnel-shaped, 1-1.5 µm long, 1-2 µm wide at the upper edge, opening 1-1.5 µm wide, periclinal thickening not observed. Conidia aggregated in heads, hyaline, smooth-walled, aseptate, cylindrical, rarely ellipsoidal, straight, sometimes slightly curved, both ends rounded, conidia of strain GLMC 1310 measure (2-)4-7(-8) × 1.5-2.5 µm, mean ± SD = 5.3 ± 1.4 × 2.0 ± 0.3 µm, L/W ratio = 2.7, while those of GLMC 1264 are longer, measuring (3-)5-8(-10) × 1.5-2 µm, mean ± SD = 6.4 ± 1.6 × 1.8 ± 0.2 µm, L/W ratio = 3.5.

Culture characteristics.

Colonies on SNA flat with an entire margin, hyaline, sometimes filter paper partly pale olivaceous to olivaceous, lacking aerial mycelium, reverse same colours, strains GLMC 1264 and GLMC 1310 5-15 mm diam., strains GLMC 517 and GLMC 1501 32-43 mm diam. in 2 wk (25 °C in the dark); Colonies on OA flat with an entire margin, buff, olivaceous buff, olivaceous to olivaceous black, lacking aerial mycelium or partly covered by pale grey aerial mycelium, reverse same colours, 28-44 mm diam. in 2 wk (25 °C in the dark).

Notes.

In total, 12 strains of C. luteo-olivacea were isolated from Prunus domestica in Baden-Württemberg (3), Lower Saxony (8) and Saxony (1). Two strains from Baden-Württemberg, two strains from Lower Saxony and the strain from Saxony had been selected for the phylogenetic analyses. The complete sequence dataset of C. luteo-olivacea , including reference strains, exhibits a variation of up to five nucleotides within ITS, up to nine nucleotides within TUB and up to 16 nucleotides within EF-1α sequences. The ITS sequences of the strains from this study are identical with those of the ex-type strain, except for GLMC 1517, which differs in five nucleotides, while all TUB sequences of our isolates differ in eight to nine nucleotides from the ex-type strain. The EF-1α sequence of all strains from this study differs in five nucleotides from the ex-type strain, except for GLMC 1264 with no differences.

Material examined.

Germany, Lower Saxony, Hollern-Twielenfleth, orchard, 53°36'13.6"N, 9°31'50.8"E, from brown wedge-shaped necrosis in wood of Prunus domestica , 8 Oct. 2015, S. Bien leg., GLM-F107114, culture GLMC 1264 = CBS 145524 = DSM 109143; Lower Saxony, Hollern-Twielenfleth, orchard, 53°36'13.6"N, 9°31'50.8"E, from brown wedge-shaped necrosis in wood of P. domestica , 8 Oct 2015, S. Bien leg., GLM-F107160, culture GLMC 1310 = CBS 145525 = DSM 109142; Saxony, in orchard north of Wölkau, 50°58'42.3"N, 13°49'40.0"E, from brown wedge-shaped necrosis in wood of P. domestica , 16 Jan 2015, S. Bien leg., GLM-F106367, culture GLMC 517; Baden-Württemberg, orchard west of Nussbach, 48°31'55.8"N, 8°00'52.4"E, from brown wedge-shaped necrosis in wood of P. domestica , 23 Aug 2016, S. Bien leg., GLM-F110581, culture GLMC 1501; Baden-Württemberg, orchard east of Nussbach, 48°31'57.3"N, 8°01'49.6"E, from brown wedge-shaped necrosis in wood of P. domestica , 23 Aug 2016, S. Bien leg., GLM-F110597, culture GLMC 1517 = CBS 145526 = DSM 109141.

Figures

Figure 5. Colony surface of analysed strains on OA medium. A Arboricolonus simplex GLMC 459 T B Cadophora africana CBS 120890 T C C. bubakii CBS 198.30 T D C. luteo-olivacea GLMC 1264 E C. novi-eboraci GLMC 1472 F C. obscura CBS 269.33 G C. prunicola CBS 120891 T H C. prunicola GLMC 1633 I C. ramosa GLMC 377 T J Minutiella pruni-avium GLMC 1624 T K Proliferodiscus ingens GLMC 1751 T L Proliferodiscus sp. GLMC 460. Cultures A, J-L after 4 wk. Cultures B-I after 2 wk. Strains with a superscript T are ex-type cultures.

Figure 9. Cadophora luteo-olivacea. A-F conidiophores and conidiogenous cells (arrow indicates a short neck) G conidia A-G from SNA A-G LM. Scale bar: 5 μm (A applies to B-G).